MafG Antibody

Images

 
Western Blot: MafG Antibody [NBP2-15019] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with MAFG antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: MAFG Antibody [NBP2-15019] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: MAFG Antibody [NBP2-15019] - Paraffin-embedded PC9 xenograft, using antibody at 1:500 dilution.
Western Blot: MAFG Antibody [NBP2-15019] - 50 ug rat muscle lysate/extract 12% SDS-PAGE gel, antibody dilution 1:1000.
Western Blot: MAFG Antibody [NBP2-15019] - Sample (30 ug of whole cell lysate) A: Hela 12% SDS PAGE; antibody diluted at 1:1000.
Western Blot: MAFG Antibody [NBP2-15019] - A.50 ug mouse muscle lysate/extract.
Immunoprecipitation: MafG Antibody [NBP2-15019] - Immunoprecipitation of MAFG protein from HeLa whole cell extracts using 5 ug of MAFG antibody. Western blot analysis was performed using MAFG antibody EasyBlot ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, MkSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

MafG Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human MAFG. The exact sequence is proprietary.
Predicted Species
Monkey (100%), Bovine (98%), Chicken (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAFG
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Rhesus Monkey (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MafG Antibody

  • basic leucine zipper transcription factor MafG
  • hMAF
  • MafG
  • MGC13090
  • MGC20149
  • transcription factor MafG
  • v-maf musculoaponeurotic fibrosarcoma (avian) oncogene family, protein G
  • v-maf musculoaponeurotic fibrosarcoma oncogene homolog G (avian)
  • V-maf musculoaponeurotic fibrosarcoma oncogene homolog G

Background

Globin gene expression is regulated through nuclear factor erythroid-2 (NFE2) elements located in enhancer-like locus control regions positioned many kb upstream of alpha- and beta-gene clusters (summarized by Blank et al., 1997 [PubMed 9166829]). NFE2 DNA-binding activity consists of a heterodimer containing a ubiquitous small Maf protein (MafF, MIM 604877; MafG; or MafK, MIM 600197) and the tissue-restricted protein p45 NFE2 (MIM 601490). Both subunits are members of the activator protein-1-like superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MAFG
Entrez
Uniprot