MafG Antibody - BSA Free

Western Blot: MafG Antibody [NBP2-15019] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with MAFG antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: MafG Antibody [NBP2-15019] - PC9 xenograft, using MAFG antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunoprecipitation: MafG Antibody [NBP2-15019] - Immunoprecipitation of MAFG protein from HeLa whole cell extracts using 5 ug of MAFG antibody. Western blot analysis was performed using MAFG antibody EasyBlot anti-Mouse IgG was used as a secondary reagent.

Western Blot: MafG Antibody [NBP2-15019] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with MafG antibody (NBP2-15019) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

MAFG antibody detects MAFG protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: MAFG protein stained by MAFG antibody (NBP2-15019) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:50.Scale bar = 10 um.

• Reactivity: Hu, Mu, Rt, Ze, Bv, Ch, RM
• Applications: WB, ICC/IF, IHC, IP, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MafG Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MafG Antibody - BSA Free (NBP2-15019) is a polyclonal antibody validated for use in IHC, WB, ICC/IF, IP and ChIP. Anti-MafG Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human MafG. The exact sequence is proprietary.
• Predicted Species: Rhesus Macaque (100%), Bovine (98%), Chicken (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAFG
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP) Assay dependent
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
• Theoretical MW: 18 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Zebrafish reactivity reported in scientific literature (PMID: 27737822).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for MafG Antibody - BSA Free

• basic leucine zipper transcription factor MafG
• hMAF
• MafG
• MGC13090
• MGC20149
• transcription factor MafG
• v-maf musculoaponeurotic fibrosarcoma (avian) oncogene family, protein G
• v-maf musculoaponeurotic fibrosarcoma oncogene homolog G (avian)
• V-maf musculoaponeurotic fibrosarcoma oncogene homolog G

Background

Globin gene expression is regulated through nuclear factor erythroid-2 (NFE2) elements located in enhancer-like locus control regions positioned many kb upstream of alpha- and beta-gene clusters (summarized by Blank et al., 1997 [PubMed 9166829]). NFE2 DNA-binding activity consists of a heterodimer containing a ubiquitous small Maf protein (MafF, MIM 604877; MafG; or MafK, MIM 600197) and the tissue-restricted protein p45 NFE2 (MIM 601490). Both subunits are members of the activator protein-1-like superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MafG Antibody (NBP2-15019)(2)

Publications using NBP2-15019

• Chan D.K. Nguyen, Benjamín A. Colón-Emeric, Shigekazu Murakami, Mia N.Y. Shujath, Chunling Yi PRMT1 promotes epigenetic reprogramming associated with acquired chemoresistance in pancreatic cancer Cell reports 2024-06-28 [PMID: 38691454]
• Sun C, Liu W, Lu Z et al. Hepatic miR-378 modulates serum cholesterol levels by regulating hepatic bile acid synthesis Theranostics 2021-02-25 [PMID: 33754066] (WB, Mouse)

Bioinformatics

• Gene Symbol: MAFG
• Uniprot:
  • O15525()