MafG Antibody

Images

 
Western Blot: MafG Antibody [NBP2-15019] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with MAFG antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: MafG Antibody [NBP2-15019] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MAFG protein stained by MAFG antibody diluted at 1:500. Red: phalloidin, a ...read more
Immunohistochemistry-Paraffin: MafG Antibody [NBP2-15019] - PC9 xenograft, using MAFG antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: MafG Antibody [NBP2-15019] - A.50 ug rat muscle lysate/extract, 12% SDS-PAGE. MAFG antibody dilution: 1:1000.The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary ...read more
Western Blot: MafG Antibody [NBP2-15019] - A.50 ug mouse muscle lysate/extract, 12% SDS-PAGE, MAFG antibody dilution: 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary ...read more
Immunoprecipitation: MafG Antibody [NBP2-15019] - Immunoprecipitation of MAFG protein from HeLa whole cell extracts using 5 ug of MAFG antibody. Western blot analysis was performed using MAFG antibody EasyBlot ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IP, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

MafG Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human MAFG. The exact sequence is proprietary.
Predicted Species
Rhesus Macaque (100%), Bovine (98%), Chicken (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAFG
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
Application Notes
Chip Assay, Chip-seq-Assay dependent
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBP2-15019 in the following applications:

  • WB
    1 publication

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:33754066). Zebrafish reactivity reported in scientific literature (PMID: 27737822).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 20% Glycerol (pH7)
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for MafG Antibody

  • basic leucine zipper transcription factor MafG
  • hMAF
  • MafG
  • MGC13090
  • MGC20149
  • transcription factor MafG
  • v-maf musculoaponeurotic fibrosarcoma (avian) oncogene family, protein G
  • v-maf musculoaponeurotic fibrosarcoma oncogene homolog G (avian)
  • V-maf musculoaponeurotic fibrosarcoma oncogene homolog G

Background

Globin gene expression is regulated through nuclear factor erythroid-2 (NFE2) elements located in enhancer-like locus control regions positioned many kb upstream of alpha- and beta-gene clusters (summarized by Blank et al., 1997 [PubMed 9166829]). NFE2 DNA-binding activity consists of a heterodimer containing a ubiquitous small Maf protein (MafF, MIM 604877; MafG; or MafK, MIM 600197) and the tissue-restricted protein p45 NFE2 (MIM 601490). Both subunits are members of the activator protein-1-like superfamily of basic leucine zipper (bZIP) proteins (see MIM 165160).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MafG Antibody (NBP2-15019)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol MAFG
Uniprot