Ribosomal protein S27 Antibody

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Western Blot: Ribosomal protein S27 Antibody [NB100-1103] - Analysis of Ribosomal protein S27 in Jurkat lysate (35ug protein in RIPA buffer) using NB100-1103 (0.05ug/ml).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Ribosomal protein S27 Antibody Summary

Immunogen
Peptide with sequence PLAKDLLHPSPEE-C corresponding to N-Terminus according to NP_001021.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RPS27
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.03 - 0.1 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Approx. 12 kDa band observed in lysates of human T-cell line Jurkat (calculated MW of 9.5 kDa band according to NP_001021.1).
Publications
Read Publication using NB100-1103.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Ribosomal protein S27 Antibody

  • Metallopan-stimulin 1
  • MPS140S ribosomal protein S27
  • MPS-1metallopanstimulin 1
  • ribosomal protein S27 (metallopanstimulin 1)
  • ribosomal protein S27

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S27E family of ribosomal proteins. It contains a C4-type zinc finger domain that can bind to zinc. The encoded protein has been shown to be able to bind to nucleic acid. It is located in the cytoplasm as a ribosomal component, but it has also been detected in the nucleus. Studies in rat indicate that ribosomal protein S27 is located near ribosomal protein S18 in the 40S subunit and is covalently linked to translation initiation factor eIF3. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol RPS27
Entrez
Uniprot