MAD1L1/MAD1 Antibody - BSA Free

Staining of human testis shows moderate positivity in nuclear membrane in cells in seminiferous ducts.

Staining of human skin shows moderate positivity in nuclear membrane in squamous epithelial cells.

Staining of human lymph node shows moderate positivity in nuclear membrane in germinal center cells.

Staining of human cerebral cortex shows strong positivity in nuclear membrane in neurons and glial cells.

Analysis in human cell line MCF-7.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MAD1L1/MAD1 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: TKVLHMSLNPTSVARQRLREDHSQLQAECERLRGLLRAMERGGTVPADLEAAAASLPSSKEVAELKKQVESAELKNQRLKEVFQTKIQEFRKACYTLTGYQIDITTENQYRLTSLYAEHPGDCLIFKATSPSGSKMQLLEIRRAPPLSNN
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAD1L1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  MAD1L1/MAD1 Protein (NBP1-89664PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for MAD1L1/MAD1 Antibody - BSA Free

• hMAD1
• HsMAD1
• MAD1 mitotic arrest deficient-like 1 (yeast)
• MAD1
• MAD1L1
• MAD1-like protein 1
• MAD1yeast, homolog-like 1
• Mitotic checkpoint MAD1 protein homolog
• PIG9
• Tax-binding protein 181
• TP53I9
• tumor protein p53 inducible protein 9
• TXBP181
• TXBP181MAD1 (mitotic arrest deficient, yeast, homolog)-like 1

Background

MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Three transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAD1L1