| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4194-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89664-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-5783-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22139-50ul | |||
![Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAD1 antibody diluted by 1:5000.](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-Western-Blot-NBP1-32775-img0011.jpg "Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAD1 antibody diluted by 1:5000.")
![Immunocytochemistry/Immunofluorescence: MAD1L1/MAD1 Antibody [NBP1-32775] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MAD1 protein stained by MAD1 antibody diluted at 1:500. Red: HEC1, a kinetochore marker, stained by HEC1 antibody [9G3.23] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32775-img0013.jpg)
![Immunohistochemistry-Paraffin: MAD1L1/MAD1 Antibody [NBP1-32775] - Paraffin-embedded SW480 Xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-Immunohistochemistry-Paraffin-NBP1-32775-img0004.jpg)
![Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775] - Sample (30 ug of whole cell lysate) A: Molt-4 7.5% SDS PAGE diluted at 1:10000](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-Western-Blot-NBP1-32775-img0005.jpg)
![Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat 2 whole cell lysate/extract 7.5% SDS-PAGE MAD1 antibody dilution: 1:3000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-Western-Blot-NBP1-32775-img0007.jpg)
![Western Blot: MAD1L1/MAD1 Antibody [NBP1-32775] - A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug C8D30 whole cell lysate/extract D. 30 ug NIH-3T3 whole cell lysate/extract E. 30 ug BCL-1 whole cell lysate/extract F. 30 ug Raw264.7 whole cell lysate/extract 7.5% SDS-PAGE MAD1 antibody dilution: 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-Western-Blot-NBP1-32775-img0009.jpg)
![Immunoprecipitation: MAD1L1/MAD1 Antibody [NBP1-32775] - Immunoprecipitation of MAD1 protein from HeLa whole cell extracts using 5 ug of MAD1 antibody. Western blot analysis was performed using MAD1 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-Immunoprecipitation-NBP1-32775-img0015.jpg)
![Western Blot: MAD1 Antibody [NBP2-17198] - A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract E. 30 ug A375 whole cell lysate/extract 7.5% SDS-PAGE antibody dilution 1:1000](http://images.novusbio.com/fullsize/MAD1-Antibody-Western-Blot-NBP2-17198-img0001.jpg "Western Blot: MAD1 Antibody [NBP2-17198] - A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract E. 30 ug A375 whole cell lysate/extract 7.5% SDS-PAGE antibody dilution 1:1000")
![Immunocytochemistry/Immunofluorescence: MAD1 Antibody [NBP2-17198] - Analysis of paraformaldehyde-fixed MCF-7, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/MAD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-17198-img0003.jpg)
![Immunohistochemistry-Paraffin: MAD1 Antibody [NBP2-17198] - Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/MAD1-Antibody-Immunohistochemistry-Paraffin-NBP2-17198-img0002.jpg)
![Immunoprecipitation: MAD1L1/MAD1 Antibody [NBP2-17198] - Immunoprecipitation of MAD1 protein from HeLa whole cell extracts using 5 ug of MAD1 antibody. Western blot analysis was performed using MAD1 antibody.](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-Immunoprecipitation-NBP2-17198-img0004.jpg)
![Western Blot: MAD1L1/MAD1 Antibody [NB100-569] - Detection of Human MAD1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAD1 antibody NB100-569 used for WB at 0.06 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-Western-Blot-NB100-569-img0014.jpg "Western Blot: MAD1L1/MAD1 Antibody [NB100-569] - Detection of Human MAD1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAD1 antibody NB100-569 used for WB at 0.06 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: MAD1L1/MAD1 Antibody [NB100-569] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-MAD1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-Immunohistochemistry-NB100-569-img0013.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunoprecipitation: MAD1L1/MAD1 Antibody [NB100-569] - Whole cell lysate (50 mcg/lane in A; 50 mcg input in B, 1 mg for IP in B) from HeLa cells. In A, HeLa cells were treated with Nocodazole (+) or mock treated (-). NB100-569 used at 0.1 mcg/ml for Western Blot (A) and at 1 mcg/mg lysate for IP (B). MAD1 was also immunoprecipitated using rabbit anti-MAD1 antibodies NB100-570 and NB100-571 using 1 mcg/mg lysate. In B, MAD1 in input and immunoprecipitate was detected using NB100-570 at 0.2 mcg/ml.](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-Immunoprecipitation-NB100-569-img0009.jpg)
![Western Blot: MAD1L1/MAD1 Antibody [NBP1-89664] - Analysis in human cell line RT-4.](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-Western-Blot-NBP1-89664-img0007.jpg "Western Blot: MAD1L1/MAD1 Antibody [NBP1-89664] - Analysis in human cell line RT-4.")
![Immunohistochemistry-Paraffin: MAD1L1/MAD1 Antibody [NBP1-89664] - Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells and glial cells.](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-Immunohistochemistry-Paraffin-NBP1-89664-img0002.jpg)
![Western Blot: MAD1L1/MAD1 Antibody (7O2C2) [NBP3-16553] - Western blot analysis of extracts of Mouse spleen, using MAD1L1/MAD1 Rabbit mAb (NBP3-16553) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-7O2C2-Western-Blot-NBP3-16553-img0001.jpg "Western Blot: MAD1L1/MAD1 Antibody (7O2C2) [NBP3-16553] - Western blot analysis of extracts of Mouse spleen, using MAD1L1/MAD1 Rabbit mAb (NBP3-16553) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western Blot: MAD1L1/MAD1 Antibody (7O2C2) [NBP3-16553] - Western blot analysis of extracts of various cell lines, using MAD1L1/MAD1 Rabbit mAb (NBP3-16553) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-7O2C2-Western-Blot-NBP3-16553-img0002.jpg)
![Western Blot: MAD1L1/MAD1 Antibody (9B10) - BSA Free [NB120-5783] - Whole extract of HeLa cells was separated on SDS-PAGE and probed with Anti-Mad1 antibody, Mouse monoclonal. The antibody was developed using 1:10000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat.Lanes:1. 1:1000 antibody2. 1:2000 antibody3. 1:4000 antibody4. Negative control](http://images.novusbio.com/fullsize/MAD1L1-MAD1-Antibody-9B10-BSA-Free-Western-Blot-NB120-5783-img0002.jpg "Western Blot: MAD1L1/MAD1 Antibody (9B10) - BSA Free [NB120-5783] - Whole extract of HeLa cells was separated on SDS-PAGE and probed with Anti-Mad1 antibody, Mouse monoclonal. The antibody was developed using 1:10000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat.Lanes:1. 1:1000 antibody2. 1:2000 antibody3. 1:4000 antibody4. Negative control")
![Western Blot: MAD1L1/MAD1 Antibody (SR2235) [NBP3-22139] - Western blot analysis using NBP3-22139 in A431 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22139_rabbit-mad1l1-mad1-mab-sr2235-672023106280.jpg "Western Blot: MAD1L1/MAD1 Antibody (SR2235) [NBP3-22139] - Western blot analysis using NBP3-22139 in A431 cell lysate.")
![Western Blot: MAD1 Overexpression Lysate (Adult Normal) [NBP2-11234] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MAD1.](http://images.novusbio.com/fullsize/MAD1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11234-img0001.jpg "Western Blot: MAD1 Overexpression Lysate (Adult Normal) [NBP2-11234] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MAD1.")
![Western Blot: MAD1 Overexpression Lysate (Adult Normal) [NBP2-11235] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MAD1.](http://images.novusbio.com/fullsize/MAD1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-11235-img0001.jpg "Western Blot: MAD1 Overexpression Lysate (Adult Normal) [NBP2-11235] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MAD1.")
![Western Blot: MAD1L1/MAD1 Antibody (JE35-54) [NBP3-32556] - Western blot analysis of MAD1L1/MAD1 on different lysates with Rabbit anti-MAD1L1/MAD1 antibody (NBP3-32556) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate Lane 2: HeLa cell lysate Lane 3: U-2 OS cell lysate Lane 4: Jurkat cell lysate Lane 5: MCF7 cell lysate Lane 6: A431 cell lysate Lane 7: A375 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 83 kDa Observed band size: 83 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32556) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32556_rabbit-mad1l1-mad1-mab-je35-54-27820241524262.jpg "Western Blot: MAD1L1/MAD1 Antibody (JE35-54) [NBP3-32556] - Western blot analysis of MAD1L1/MAD1 on different lysates with Rabbit anti-MAD1L1/MAD1 antibody (NBP3-32556) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate Lane 2: HeLa cell lysate Lane 3: U-2 OS cell lysate Lane 4: Jurkat cell lysate Lane 5: MCF7 cell lysate Lane 6: A431 cell lysate Lane 7: A375 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 83 kDa Observed band size: 83 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32556) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")