M-CSFR/CD115 Antibody

Western Blot: M-CSF R Antibody [NBP1-52071] - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CSF1R with C-terminal MYC tag probed with EB10258 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with EB10258 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

Product Discontinued

M-CSFR/CD115 Antibody Summary

• Immunogen: Peptide with sequence C-NDSFSEQDLDK corresponding to internal region according to NP_005202.2.
• Epitope: C-NDSFSEQDLDK
• Predicted Species: Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CSF1R
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.2 - 1 ug/ml
• Application Notes: WB: In transfected HEK293 transiently expressing full-length human CSF1R (myc and DYKDDDDK tagged), a band of approx. 200 kDa band was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for M-CSFR/CD115 Antibody

• CD115 antigen
• CD115
• c-fms
• colony stimulating factor 1 receptor
• CSF1R
• CSF-1-R
• CSFR
• EC 2.7.10.1
• FMS proto-oncogene
• FMSFIM2
• macrophage colony stimulating factor I receptor
• macrophage colony-stimulating factor 1 receptor
• McDonough feline sarcoma viral (v-fms) oncogene homolog
• M-CSF R
• MCSFR
• M-CSFR
• Proto-oncogene c-Fms

Background

MCSF Receptor is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a member of the CSF1/PDGF receptor family of tyrosine protein kinases and contains 5 immunoglobulin like C2 type domains. CD115 is expressed by cells of the monocytic lineage and by progenitor cells. Mutations in this gene have been associated with a predisposition to myeloid malignancy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for M-CSFR/CD115 Antibody (NBP1-52071)(1)

Publication using NBP1-52071

• Woo HH, Yi X, Lamb T et al. Posttranscriptional suppression of proto-oncogene c-fms expression by vigilin in breast cancer. Mol Cell Biol 2011-01-01 [PMID: 20974809]

Bioinformatics

• Gene Symbol: CSF1R
• Entrez:
  • 1436(Human)
  • 307403(Rat)
• Uniprot:
  • NP_005202.2(Human)