M-CSFR/CD115 Antibody (460630) [Alexa Fluor® 594]

• Reactivity: Mu
• Applications: WB
• Clone: 460630
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 594

M-CSFR/CD115 Antibody (460630) [Alexa Fluor® 594] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse M-CSF R/CD115
  Ala20-Ser511
  Accession # P09581
• Specificity: Detects mouse M-CSF R/CD115 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human
  M-CSF R/CD115, recombinant mouse (rm) PDGF R alpha, rmPDGF R beta, or rmFlt-3 Ligand is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
• Purity: Protein A or G purified

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for M-CSFR/CD115 Antibody (460630) [Alexa Fluor® 594]

• CD115 antigen
• CD115
• c-fms
• colony stimulating factor 1 receptor
• CSF1R
• CSF-1-R
• CSFR
• EC 2.7.10.1
• FMS proto-oncogene
• FMSFIM2
• macrophage colony stimulating factor I receptor
• macrophage colony-stimulating factor 1 receptor
• McDonough feline sarcoma viral (v-fms) oncogene homolog
• M-CSF R
• MCSFR
• M-CSFR
• Proto-oncogene c-Fms

Background

M-CSF receptor (M-CSF R), also called CSF1 R, is the product of the c-fms proto-oncogene. It is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region (1-4). M-CSF receptor is expressed primarily on cells of the monocyte/macrophage lineage, dendritic cells, stem cells and in the developing placenta (1). Mouse M-CSF receptor cDNA encodes a 977 amino acid (aa) type I membrane protein with a 19 aa signal peptide, a 492 aa extracellular region containing the ligand-binding domain, a 25 aa transmembrane domain and a 441 aa cytoplasmic domain. The mouse M-CSF R ECD shares > 99% aa identity with rat and 60-63% aa identity with corresponding sequences in human, canine, feline and bovine M-CSF R. Activators of protein kinase C induce TACE/ADAM17 cleavage of the M-CSF receptor, releasing the functional ligand-binding extracellular domain (5). M-CSF binding induces receptor homodimerization, resulting in transphosphorylation of specific cytoplasmic tyrosine residues and signal transduction (6). The intracellular domain of activated M-CSF R binds more than 150 proteins that affect cell proliferation, survival, differentiation and cytoskeletal reorganization. Among these, PI3Kinase, P42/44 ERK and c-Cbl are key transducers of M-CSF R signals (3, 4). M-CSF R engagement is continuously required for macrophage survival and regulates lineage decisions and maturation of monocytes, macrophages, osteoclasts and DC (3, 4). M-CSF R and integrin alpha _v beta ₃ share signaling pathways during osteoclastogenesis, and deletion of either causes osteopetrosis (7, 8). In the brain, microglia expressing increased M-CSF R are concentrated with Alzheimers a beta peptide, but their role in pathogenesis is unclear (9, 10).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.