M-CSF Antibody (2D10)

Western Blot: M-CSF Antibody (2D10) [NBP1-47472] - Analysis using CSF1 mouse mAb against human recombinant CSF2 (1) and CSF1 (2).

Immunohistochemistry-Paraffin: M-CSF Antibody (2D10) [NBP1-47472] - Analysis of human Placenta tissues using anti-CSF1 mouse mAb.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 2D10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

M-CSF Antibody (2D10) Summary

• Immunogen: Purified recombinant fragment of human M-CSF expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CSF1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for M-CSF Antibody (2D10)

• colony stimulating factor 1 (macrophage)
• CSF1
• CSF-1
• Lanimostim
• macrophage colony stimulating factor
• macrophage colony-stimulating factor 1
• MCSF
• M-CSF
• MCSFlanimostim
• MGC31930

Background

Four distinct colony-stimulating factors (CSFs) that promote survival, proliferation and differentiation of bone marrow precursor cells have been well characterized: granulocyte macrophage CSF (GMCSF), granulocyte CSF (GCSF), macrophage CSF (MCSF), and Interleukin-3 (IL-3, Multi CSF). Both GMCSF and IL-3 are multipotential growth factors, stimulating proliferation of progenitor cells from more than one hematopoietic lineage. In contrast, GCSF and MCSF are lineage restricted hematopoietic growth factors, stimulating final mitotic divisions and the terminal cellular maturation of the partially differentiated hematopoietic progenitors.Macrophage CSF, also known as CSF1, is produced by monocytes, fibroblasts and endothelial cells. It stimulates the formation of macrophage colonies, enhances antibody-dependent, cell-mediated cytotoxicity by monocytes and macrophages, and inhibits bone resorption by osetoclasts. Natural human MCSF is a dimeric glycoprotein of 70-90 kD molecular weight, existing in multiple glycosylation forms. It binds to a 165 kD glycoprotein of the receptor tyrosine kinase subclass III, a family that includes the receptors for platelet derived growth factor (PDGF) and stem cell factor (SCF).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for M-CSF Antibody (NBP1-47472)(2)

Publications using NBP1-47472

• Gerngross L, Lehmicke G, Belkadi A, Fischer, T. Role for cFMS in maintaining alternative macrophage polarization in SIV infection: implications for HIV neuropathogenesis J Neuroinflammation 2015-04-18 [PMID: 25886134] (IHC-P)
• Gerngross L, Fischer T. Evidence for cFMS signaling in HIV production by brain macrophages and microglia. J. Neurovirol. 2014-01-01 [PMID: 25060299]

Bioinformatics

• Gene Symbol: CSF1