LYVE-1 Antibody (ALY7) [Biotin]

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Flow Cytometry: LYVE-1 Antibody (ALY7) [Biotin] [NBP1-43645] - Staining of LYVE-1 transfected cells with 0.06 ug of Rat IgG1 k Isotype Control Biotin (blue histogram) or 0.06 ug of Anti-Mouse Lyve-1 Biotin (purple ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IHC, IHC-Fr
Clone
ALY7
Clonality
Monoclonal
Host
Rat
Conjugate
Biotin
Concentration
0.5 mg/ml

LYVE-1 Antibody (ALY7) [Biotin] Summary

Immunogen
The immunogen for this antibody is mouse LYVE1.
Marker
Lymphatic Vessel Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
LYVE1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry < 0.125 ug/10^6 cells
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
Application Notes
This ALY7 antibody has been tested by flow cytometric analysis of transfected cell line or immunofluorescent microscopy of cryosections of mouse intestine. This can be used at less than or equal to 0.125 ug per million cells in a 100 ul total staining volume for flow cytometry and 2.5 ug/ml for immunofluorescent micoscopy. Immunocytochemistry/Immunofluorescence was reported in scientific literature.
Readout System

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for LYVE-1 Antibody (ALY7) [Biotin]

  • cell surface retention sequence binding protein-1
  • Cell surface retention sequence-binding protein 1
  • CRSBP1
  • CRSBP-1
  • extracellular link domain containing 1
  • extracellular link domain-containing 1
  • Extracellular link domain-containing protein 1
  • HAR
  • Hyaluronic acid receptor
  • lymphatic vessel endothelial hyaluronan receptor 1
  • lymphatic vessel endothelial hyaluronic acid receptor 1
  • LYVE1
  • LYVE-1
  • LYVE-1XLKD1
  • XLKD1

Background

The monoclonal antibody ALY7 recognizes mouse LYVE-1, a transmembrane glycoprotein with similarity to CD44. The extracellular domain contains a conserved hyaluronan binding domain also found in CD44. Expression is found on lymphatic and liver endothelial cells and some populations of macrophages. The lymphatic system is responsible for transporting proteins and cells (especially dendritic cells) to tissues throughout the body, thereby acting as immune surveyors. LYVE-1 is one characteristic protein, along with podoplanin, PROX-1, Tie-2 and VEGFR-3, that is expressed on lymphatic endothelial cells (LECS). The ligand for LYVE-1 is hyaluronan, a large mucopolysaccharide. Although LYVE-1 can bind hyaluronan in vitro, the site for ligand binding in vivo is masked by sialyated O-linked glycan chains. It is postulated that binding to ligand requires modification/unmasking to expose the binding site. The development and remodeling of the endothelium after injury is an area of extensive study. When transplanted, hematopoietic stem cells (HSCs) can give rise to LECs that integrate into the endothelium in normal and metastatic tissue.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol LYVE1
Entrez