LYVE-1 Antibody (ALY7)

Images

 
Immunohistochemistry: LYVE-1 Antibody (ALY7) [NBP1-43411] - Immunohistochemistry of cryosections of mouse intestine at 2.5 ug/ml of anti-mouse LYVE-1 antibody followed by Anti-Rat IgG Rhodamine (right). Phase image of ...read more
Flow Cytometry: LYVE-1 Antibody (ALY7) [NBP1-43411] - Analysis using the Biotin conjugate of NBP1-43411. Staining of LYVE-1 transfected cells with 0.06 ug of Rat IgG1 k Isotype Control Biotin (blue histogram) or 0.06 ug ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr
Clone
ALY7
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

LYVE-1 Antibody (ALY7) Summary

Immunogen
The immunogen for this antibody is mouse LYVE1.
Marker
Lymphatic Vessel Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
LYVE1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry <= 0.125 ug/10^6 cells in 100 ul
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 2.5 ug/ml
Application Notes
This ALY7 antibody has been tested by flow cytometric analysis of transfected cell line or immunofluorescent microscopy of cryosections of mouse intestine. This can be used at less than or equal to 0.125 ug per million cells in a 100 ul total staining volume for flow cytometry and 2.5 ug/ml for immunofluorescent micoscopy. Immunocytochemistry/Immunofluorescence was reported in scientific literature.
Publications
Read Publications using
NBP1-43411 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS, pH 7.2
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for LYVE-1 Antibody (ALY7)

  • cell surface retention sequence binding protein-1
  • Cell surface retention sequence-binding protein 1
  • CRSBP1
  • CRSBP-1
  • extracellular link domain containing 1
  • extracellular link domain-containing 1
  • Extracellular link domain-containing protein 1
  • HAR
  • Hyaluronic acid receptor
  • lymphatic vessel endothelial hyaluronan receptor 1
  • lymphatic vessel endothelial hyaluronic acid receptor 1
  • LYVE1
  • LYVE-1
  • LYVE-1XLKD1
  • XLKD1

Background

The monoclonal antibody ALY7 recognizes mouse LYVE-1, a transmembrane glycoprotein with similarity to CD44. The extracellular domain contains a conserved hyaluronan binding domain also found in CD44. Expression is found on lymphatic and liver endothelial cells and some populations of macrophages. The lymphatic system is responsible for transporting proteins and cells (especially dendritic cells) to tissues throughout the body, thereby acting as immune surveyors. LYVE-1 is one characteristic protein, along with podoplanin, PROX-1, Tie-2 and VEGFR-3, that is expressed on lymphatic endothelial cells (LECS). The ligand for LYVE-1 is hyaluronan, a large mucopolysaccharide. Although LYVE-1 can bind hyaluronan in vitro, the site for ligand binding in vivo is masked by sialyated O-linked glycan chains. It is postulated that binding to ligand requires modification/unmasking to expose the binding site. The development and remodeling of the endothelium after injury is an area of extensive study. When transplanted, hematopoietic stem cells (HSCs) can give rise to LECs that integrate into the endothelium in normal and metastatic tissue.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LYVE-1 Antibody (NBP1-43411)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ICC/IF, IHC, IHC-Fr.


Filter By Application
ICC/IF
(2)
IHC
(1)
IHC-Fr
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(2)
All Species
Showing Publications 1 - 9 of 9.
Publications using NBP1-43411 Applications Species
Klotz L, Norman S, Vieira JM et al. Cardiac lymphatics are heterogeneous in origin and respond to injury. Nature. 2015 May 20 [PMID: 25992544] (Mouse) Mouse
Harrell JC, Pfefferle AD, Zalles N et al. Endothelial-like properties of claudin-low breast cancer cells promote tumor vascular permeability and metastasis. Clin Exp Metastasis. 2013 Aug 22 [PMID: 23975155] (IHC-Fr, ICC/IF, Human) IHC-Fr, ICC/IF Human
Mirza M, Pang MF, Zaini MA, Haiko P, Tammela T, Alitalo K, Philipson L, Fuxe J, Sollerbrant K. Essential Role of the Coxsackie - and Adenovirus Receptor (CAR) in Development of the Lymphatic System in Mice. PLoS One;7(5):e37523. 2012 [PMID: 22624044] (IHC, ICC/IF, Mouse) IHC, ICC/IF Mouse
Harada K, Yamazaki T, Iwata C, Yoshimatsu Y, Sase H, Mishima K, Morishita Y, Hirashima M, Oike Y, Suda T, Miura N, Watabe T, Miyazono K. Identification of targets of Prox1 during in vitro vascular differentiation from embryonic stem cells: functional roles of HoxD8 in lymphangiogenesis. J Cell Sci;122(Pt 21):3923-30. 2009 Nov 1. [PMID: 19825936]
Luong MX, Tam J, Lin Q, Hagendoorn J, Moore KJ, Padera TP, Seed B, Fukumura D, Kucherlapati R, Jain RK. Lack of lymphatic vessel phenotype in LYVE-1/CD44 double knockout mice. J Cell Physiol;219(2):430-7. 2009 May. [PMID: 19170073]
Jiang S, Bailey AS, Goldman DC, Swain JR, Wong MH, Streeter PR, Fleming WH. Hematopoietic stem cells contribute to lymphatic endothelium. PLoS One;3(11):e3812. 2008 [PMID: 19043576]
Hirashima M, Sano K, Morisada T, Murakami K, Rossant J, Suda T. Lymphatic vessel assembly is impaired in Aspp1-deficient mouse embryos. Dev Biol;316(1):149-59. 2008 Apr 1. [PMID: 18304521]
Mishima K, Watabe T, Saito A, Yoshimatsu Y, Imaizumi N, Masui S, Hirashima M, Morisada T, Oike Y, Araie M, Niwa H, Kubo H, Suda T, Miyazono K. Prox1 induces lymphatic endothelial differentiation via integrin alpha9 and other signaling cascades. Mol Biol Cell;18(4):1421-9. 2007 Apr. [PMID: 17287396]
Hamaguchi I, Morisada T, Azuma M, Murakami K, Kuramitsu M, Mizukami T, Ohbo K, Yamaguchi K, Oike Y, Dumont DJ, Suda T. Loss of Tie2 receptor compromises embryonic stem cell-derived endothelial but not hematopoietic cell survival. Blood;107(3):1207-13. 2006 Feb 1. [PMID: 16219799]

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-43411AF405
Alexa Fluor 488 Labeled NBP1-43411AF488
Alexa Fluor 647 Labeled NBP1-43411AF647
Alexa Fluor 700 Labeled NBP1-43411AF700
Allophycocyanin Labeled NBP1-43411APC
Biotin Labeled NBP1-43645
DyLight 350 Labeled NBP1-43411UV
DyLight 405 Labeled NBP1-43411V
DyLight 488 Labeled NBP1-43411G
DyLight 680 Labeled NBP1-43411FR
DyLight 755 Labeled NBP1-43411IR
PE Labeled NBP1-43411PE
PerCP Labeled NBP1-43411PCP

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Bioinformatics

Gene Symbol LYVE1
Entrez
Uniprot