Lysyl Oxidase Homolog 2/LOXL2 Antibody

Western Blot: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP2-76946] - Western blot analysis of LOXL2 on A549 and A431 cell lysate using anti-LOXL2 antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP2-76946] - ICC staining LOXL2 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP2-76946] - Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-LOXL2 antibody. Counter stained with hematoxylin. The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

Flow Cytometry: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP2-76946] - Flow cytometric analysis of A549 cells with LOXL2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP2-76946] - ICC staining LOXL2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP2-76946] - Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-LOXL2 antibody. Counter stained with hematoxylin. The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

Immunohistochemistry: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP2-76946] - Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-LOXL2 antibody. Counter stained with hematoxylin. The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

Immunohistochemistry: Lysyl Oxidase Homolog 2/LOXL2 Antibody [NBP2-76946] - Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-LOXL2 antibody. Counter stained with hematoxylin. The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.

Product Discontinued

Lysyl Oxidase Homolog 2/LOXL2 Antibody Summary

• Immunogen: Recombinant protein within human Lysyl Oxidase Homolog 2/LOXL2 aa 300-480. (SwissProt: Q9Y4K0 Human; SwissProt: P58022 Mouse; SwissProt: B5DF27 Rat)
• Localization: Endoplasmic reticulum. Nucleus. Secreted.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LOXL2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Theoretical MW: 87 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Lysyl Oxidase Homolog 2/LOXL2 Antibody

• EC 1.4.3
• EC 1.4.3.-
• LOL2
• LOR2
• LOXL2
• Lysyl Oxidase Homolog 2
• lysyl oxidase related 2
• lysyl oxidase-like 2
• Lysyl oxidase-like protein 2
• Lysyl oxidase-related protein 2
• Lysyl oxidase-related protein WS9-14
• WS9-14

Background

LOXL2 encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LOXL2