SUZ12 Antibody (448909) [Unconjugated]

Images

 
Western blot shows lysates of JEG‑3 human epithelial choriocarcinoma cell line. Gels were loaded with 40 µg of cytoplasmic (Cyto) and 20 µg of nuclear (Nuc) extracts. PVDF membrane was probed with 1 µg/mL of Mouse ...read more
SUZ12 was detected in immersion fixed D3 mouse embryonic stem cell line using Mouse Anti-Human/Mouse SUZ12 Monoclonal Antibody (Catalog # MAB4184) at 10 µg/mL for 3 hours at room temperature. Cells were stained ...read more
SUZ12 was immunoprecipitated from 400 µg of HeLa human cervical epithelial carcinoma cell line lysates using 4 µg of Mouse Anti-Human/Mouse SUZ12 Monoclonal Antibody (Catalog # MAB4184) coated on 4 wells of a 96 well ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP, ICC/IF
Clone
448909
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

SUZ12 Antibody (448909) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human SUZ12
Thr131-Asn305
Accession # Q15022
Specificity
Detects human SUZ12 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SUZ12
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Immunoprecipitation 4 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publication using
MAB4184 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SUZ12 Antibody (448909) [Unconjugated]

  • ChET 9 protein
  • CHET9
  • CHET9polycomb protein SUZ12
  • Chromatin precipitated E2F target 9 protein
  • JJAZ1
  • JJAZ1Joined to JAZF1 protein
  • KIAA0160Suppressor of zeste 12 protein homolog
  • suppressor of zeste 12 homolog (Drosophila)
  • SUZ12

Background

SUZ12 (suppressor of zeste 12; also known as Joined to JazF1) is a 90-95 kDa member of the Polycomb group of transcriptional regulators. It is part of a transcription repression complex termed PRC2 (Polycomb repressive complex 2) that methylates histones in the nucleus, resulting in homeotic (pattern-inducing) gene silencing. Human SUZ12 is 739 amino acids (aa) in length. It contains one C2H2-type zinc finger region (aa 44-8471) and a C-terminal VEFS-box (aa 563-639). There is one potential splice form that shows a deletion of 51 aa over aa 129-197. Human SUZ12 shares 90% and 99% aa identity with mouse and dog SUZ12, respectively. Over the immunogen sequence in MAB4184, aa identity between human and mouse is 99%.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SUZ12 Antibody (MAB4184)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Western Blot
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SUZ12
Uniprot