| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-06640SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-82107SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06855SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92982-0.02ml | |||
NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="Lysine (K)-specific Demethylase 6B/KDM6B was detected in immersion fixed BG01V human embryonic stem cells, differentiated (upper panel) and undifferentiated (lower panel) using Goat Anti-Human Lysine (K)-specific Demethylase 6B/KDM6B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7300) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF7300-SP | |||
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP1-06640] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-JMJD3 [NBP1-06640] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-6B-KDM6B-JMJD3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-06640-img0005.jpg)
![Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP1-06640] - Analysis of JMJD3 expression on HepG2 whole cell lysates.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-6B-KDM6B-JMJD3-Antibody-Western-Blot-NBP1-06640-img0004.jpg)
![Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP1-06640] - Total protein from human HepG2 and mouse 3T3 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-JMJD3 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-6B-KDM6B-JMJD3-Antibody-Western-Blot-NBP1-06640-img0006.jpg)
![Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free [NBP1-06640] - KDM6A protein levels are decreased following EMT & upregulated during MET(A) HMLE cells were transduced with either an empty vector (HMLE-Vector), or genes encoding the EMT-inducing transcription factors Snail (HMLE-Snail) or Twist (HMLE-Twist) [4]. Whole-cell lysates were analyzed by immunoblotting for KDM6A. beta -actin was used as a loading control. (B-F) MCF10A cells were treated with sterile 4 mM HCl containing 0.1% bovine serum albumin (Vehicle) or TGFB for 5 days (TGFB+). In parallel, MCF10A cells were exposed to TGFB for 5 days in order to induce EMT, & subsequently subjected to TGFB-withdrawal, for a further 5 days, to elicit MET (TGFB+/-). (B-C) MCF10A cells, treated as described, were fixed & immunostained for E-cadherin (B) & N-cadherin (C). Nuclei were counterstained with DAPI (blue). Scale bars, 50 μm. (D) MCF10A cells, treated as described, were analyzed by immunoblotting for KDM6B & vimentin (Vim). COX IV was used as a loading control. (E) MCF10A cells, treated as described, were fixed & immunostained for KDM6A. Nuclei were counterstained with DAPI (blue). Scale bars, 50 μm. (F) Cells were analyzed by immunoblotting for KDM6A, EZH2 & RING1A. beta -actin was used as a loading control. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.19214), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-06640_rabbit-polyclonal-lysine-k-specific-demethylase-6b-kdm6b-jmjd3-antibody-310202416163738.jpg)
![Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP2-82107] - Western Blot Validation in HepG2 Cell Lysate. Loading: 15 ug of lysates per lane. Antibodies: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3, NBP2-82107, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.25 ug/mL Lane 2: 0.5 ug/mL Lane 3: 0.25 ug/mL with blocking peptide](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-6B-KDM6B-JMJD3-Antibody-Western-Blot-NBP2-82107-img0007.jpg "Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP2-82107] - Western Blot Validation in HepG2 Cell Lysate. Loading: 15 ug of lysates per lane. Antibodies: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3, NBP2-82107, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.25 ug/mL Lane 2: 0.5 ug/mL Lane 3: 0.25 ug/mL with blocking peptide")
![Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP2-82107] - Western Blot Validation in Human, Mouse, and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 [NBP2-82107], (1 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-6B-KDM6B-JMJD3-Antibody-Western-Blot-NBP2-82107-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP2-82107] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane. Antibodies: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 NBP2-82107 (1 ug/mL), JMJD3 from Company B, (2 ug/mL) and beta-actin NBP1-76692 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-6B-KDM6B-JMJD3-Antibody-Western-Blot-NBP2-82107-img0003.jpg)
![Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP2-82107] - Western Blot Validation in HeLa Cell Lysate. Loading: 15 ug of lysates per lane. Antibodies: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3, NBP2-82107 (0.25 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-6B-KDM6B-JMJD3-Antibody-Western-Blot-NBP2-82107-img0004.jpg)
![Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP2-82107] - Western Blot Validation in K562 Cell Lysate. Loading: 15 ug of lysates per lane. Antibodies: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3, NBP2-82107 (0.5 ug/mL) , 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: in the absence and Lane 2: in the presence of blocking peptide.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-6B-KDM6B-JMJD3-Antibody-Western-Blot-NBP2-82107-img0005.jpg)
![Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP2-82107] - Western Blot Validation in Mouse A-20 Cell Lysate. Loading: 15 ug of lysates per lane. Antibodies: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3, NBP2-82107 (0.5 ug/mL) , 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: in the absence and Lane 2: in the presence of blocking peptide](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-6B-KDM6B-JMJD3-Antibody-Western-Blot-NBP2-82107-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP2-82107] - Immunocytochemistry Validation of JMJD3 in K562 Cells. Immunocytochemical analysis of K562 cells using Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 antibody (NBP2-82107) at 2.5 ug/ml. Cells were fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-6B-KDM6B-JMJD3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-82107-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody - BSA Free [NBP2-82107] - Validation of Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 in K562 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).](http://images.novusbio.com/fullsize/nbp2-82107_rabbit-lysine-k-specific-demethylase-6b-kdm6b-jmjd3-pab-271120241937784.jpg)
![Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP3-06855] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-JMJD3 NBP3-06855 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-6B-KDM6B-JMJD3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-06855-img0002.jpg "Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP3-06855] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-JMJD3 NBP3-06855 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP3-06853] - Analysis of a FFPE tissue section of human stomach using 1:200 dilution of JMJD3 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-6B-KDM6B-JMJD3-Antibody-Immunohistochemistry-Paraffin-NBP3-06853-img0001.jpg)
![Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP2-92982] - Analysis of extracts of mouse brain, using Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-6B-KDM6B-JMJD3-Antibody-Western-Blot-NBP2-92982-img0003.jpg "Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP2-92982] - Analysis of extracts of mouse brain, using Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST")
![Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP2-92982] - Analysis of U2OS cells using Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-6B-KDM6B-JMJD3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92982-img0002.jpg)
![Western Blot: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP2-92982] - Analysis of extracts of various cell lines, using Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 .Exposure time: 90s.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-6B-KDM6B-JMJD3-Antibody-Western-Blot-NBP2-92982-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 Antibody [NBP2-92982] - Analysis of NIH/3T3 cells using Lysine (K)-specific Demethylase 6B/KDM6B/JMJD3 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-6B-KDM6B-JMJD3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92982-img0001.jpg)
