Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (3G10C2) - BSA Free

Western Blot: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (3G10C2) [NBP2-61819] - Analysis using KDM3A mAb against human KDM3A (AA: 311-445) recombinant protein. (Expected MW is 40.3 kDa)

Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (3G10C2) [NBP2-61819] - Analysis of Hela cells using KDM3A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.

Flow Cytometry: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (3G10C2) [NBP2-61819] - Analysis of Hela cells using KDM3A mouse mAb (green) and negative control (red).

Western Blot: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (3G10C2) [NBP2-61819] - Analysis using KDM3A mAb against HEK293 (1) and KDM3A (AA: 311-445)-hIgGFc transfected HEK293 (2) cell lysate.

ELISA: Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (3G10C2) [NBP2-61819] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: 3G10C2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (3G10C2) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A (AA: 311-445) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KDM3A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/ Immunofluorescence 1:50-1:250
  • Western Blot 1:500-1:2000
• Theoretical MW: 105.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Lysine (K)-specific Demethylase 3A/KDM3A/JMJD1A Antibody (3G10C2) - BSA Free

• EC 1.14.11
• EC 1.14.11.-
• JHDM2A
• JHDM2AJMJD1AJHMD2A
• JmjC domain-containing histone demethylation protein 2A
• JMJD1
• JMJD1A
• jumonji C domain-containing histone demethylase 2A
• jumonji domain containing 1
• jumonji domain containing 1A
• Jumonji domain-containing protein 1A
• KDM3A
• KIAA0742
• KIAA0742DKFZp686A24246
• Lysine (K)specific Demethylase 3A
• Lysine (K)-specific Demethylase 3A
• lysine-specific demethylase 3A
• testis-specific protein A
• TSGA
• TSGADKFZp686P07111

Background

This gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. Alternative splicing results in multiple transcript variants.;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KDM3A