LYPLA3 Antibody

Western Blot: LYPLA3 Antibody [NBP2-17193] - Sample (30 ug of whole cell lysate) A: Jurkat 10% SDS PAGE gel, diluted at 1:2000.

Immunocytochemistry/Immunofluorescence: LYPLA3 Antibody [NBP2-17193] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: LYPLA3 Antibody [NBP2-17193] - Human colon carcinoma, using LYPLA3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Product Discontinued

LYPLA3 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human LYPLA3. The exact sequence is proprietary.
• Localization: Lysosome (By similarity); Secreted
• Predicted Species: Mouse (92%), Rat (91%), Canine (96%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PLA2G15
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Bovine (85%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris-Glycine (pH7), 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for LYPLA3 Antibody

• 1-O-acylceramide synthase
• ACSDKFZp564A0122
• EC 2.3.1.43
• GXVPLA2
• LLPLLCAT-like lysophospholipase
• LPLA2group XV phospholipase A2
• LYPLA3EC 2.3.1.-
• lysophospholipase 3 (lysosomal phospholipase A2)
• Lysophospholipase 3
• Lysosomal phospholipase A2
• phospholipase A2, group XV

Background

Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. The protein encoded by this gene hydrolyzes lysophosphatidylcholine to glycerophosphorylcholine and a free fatty acid. This enzyme is present in the plasma and thought to be associated with high-density lipoprotein. A later paper contradicts the function of this gene. It demonstrates that this gene encodes a lysosomal enzyme instead of a lysophospholipase and has both calcium-independent phospholipase A2 and transacylase activities. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for LYPLA3 Antibody (NBP2-17193)(1)

Publication using NBP2-17193

• Jain R, Geoghegan G, Davidson J et al. Modulation of hepatic transcription factor EB activity during cold exposure uncovers direct regulation of bis(monoacylglycero)phosphate lipids by Pla2g15 bioRxiv : the preprint server for biology 2023-11-10 [PMID: 37986778]

Bioinformatics

• Gene Symbol: PLA2G15
• Uniprot:
  • Q8NCC3(Human)