Lymphotoxin beta R/TNFRSF3 Antibody [Unconjugated]

NIH‑3T3 mouse embryonic fibroblast cell line was stained with Goat Anti-Mouse Lymphotoxin beta R/TNFRSF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1008, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Lymphotoxin beta R/TNFRSF3 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Lymphotoxin beta R/TNFRSF3
  Ser28-Pro218
  Accession # P50284
• Specificity: Detects mouse Lymphotoxin beta R/TNFRSF3 in direct ELISA and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) LT beta R is observed, and less than 1% cross-reactivity with recombinant mouse (rm) 4-1BB, rmCD27, rmOPG, rmRANK, rmCD30, rmCD40, rhDR6, rmTNF RI, rmTNF RII, rmEDAR, rmFas and rmGITR is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LTBR
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lymphotoxin beta R/TNFRSF3 Antibody [Unconjugated]

• CD18
• D12S370
• ltbetar
• LT-BETA-R
• LTBR
• lymphotoxin B receptor
• Lymphotoxin beta R
• lymphotoxin beta receptor (TNFR superfamily, member 3)
• Lymphotoxin-beta receptor
• LymphotoxinbR
• TNF RIII
• TNF Rrp
• TNFCRTNF-RIII
• TNFR superfamily, member 3
• TNFR2-RP
• TNFR3
• TNF-R-III
• TNFR-RP
• TNFRSF3
• TNFRSF3TNFR-III
• Tumor necrosis factor C receptor
• Tumor necrosis factor receptor 2-related protein
• tumor necrosis factor receptor superfamily member 3
• tumor necrosis factor receptor superfamily, member 3
• Tumor necrosis factor receptor type III

Background

Lymphotoxin beta R is a member of the TNF receptor superfamily and is now designated TNFRSF3. Lymphotoxin beta R transduces signals following binding of LIGHT or the heterotrimeric Lymphotoxins LT alpha 1/ beta 2 or LT alpha 2/ beta 1. It plays a critical role in controlling cellular immune functions and lymphoid organogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lymphotoxin beta R/TNFRSF3 Antibody (AF1008)(7)

Publications using AF1008

• L Marrero, J Simkin, M Sammarco, K Muneoka Fibroblast reticular cells engineer a blastema extracellular network during digit tip regeneration in mice Regeneration (Oxf), 2017;4(2):69-84. 2017 [PMID: 28616246] (Bioassay, Mouse)
• Kumar V, Dasoveanu D, Chyou S, Tzeng T, Rozo C, Liang Y, Stohl W, Fu Y, Ruddle N, Lu T A dendritic-cell-stromal axis maintains immune responses in lymph nodes. Immunity, 2015;42(4):719-30. 2015 [PMID: 25902483] (Neutralization, Mouse)
• Katakai T, Kondo N, Ueda Y, Kinashi T Autotaxin produced by stromal cells promotes LFA-1-independent and Rho-dependent interstitial T cell motility in the lymph node paracortex. J Immunol, 2014;193(2):617-26. 2014 [PMID: 24935929] (Functional Assay, Mouse)
• Baluk P, Yao LC, Feng J, Romano T, Jung SS, Schreiter JL, Yan L, Shealy DJ, McDonald DM TNF-alpha drives remodeling of blood vessels and lymphatics in sustained airway inflammation in mice. J. Clin. Invest., 2009;119(10):2954-64. 2009 [PMID: 19759514] (IHC-Fr, Mouse)
• Suto H, Katakai T, Sugai M, Kinashi T, Shimizu A CXCL13 production by an established lymph node stromal cell line via lymphotoxin-beta receptor engagement involves the cooperation of multiple signaling pathways. Int. Immunol., 2009;21(4):467-76. 2009 [PMID: 19251935] (Neutralization, Mouse)
• Katakai T, Suto H, Sugai M, Gonda H, Togawa A, Suematsu S, Ebisuno Y, Katagiri K, Kinashi T, Shimizu A Organizer-like reticular stromal cell layer common to adult secondary lymphoid organs. J. Immunol., 2008;181(9):6189-200. 2008 [PMID: 18941209] (Bioassay, Mouse)
• Katakai T, Hara T, Sugai M, Gonda H, Shimizu A Lymph node fibroblastic reticular cells construct the stromal reticulum via contact with lymphocytes. J. Exp. Med., 2004;200(6):783-95. 2004 [PMID: 15381731] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: LTBR
• Uniprot:
  • P50284()