LYAG/GAA Antibody

Images

 
Western Blot: LYAG/GAA Antibody [NBP1-69295] - This Anti-GAA antibody was used in Western Blot of MCF7 tissue lysate at a concentration of 1ug/ml.
Immunocytochemistry/ Immunofluorescence: LYAG/GAA Antibody [NBP1-69295] - LYAG was probed in human fibroblasts fixed with 4% PFA. Cells were permeabilized and blocked in 0.1% Tween and 1% Bovine serum albumin. Anti-LYAG ...read more
Immunocytochemistry/ Immunofluorescence: LYAG/GAA Antibody [NBP1-69295] - Antibody Formalin Fixed Paraffin Embedded Tissue: Human Lung Tissue Observed Staining: Cytoplasmic in alveolar type I cells

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

LYAG/GAA Antibody Summary

Immunogen
Synthetic peptides corresponding to GAA(glucosidase, alpha; acid ) The peptide sequence was selected from the N terminal of GAA. Peptide sequence FGVIVRRQLDGRVLLNTTVAPLFFADQFLQLSTSLPSQYITGLAEHLSPL.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GAA
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.2-1 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
Application Notes
This is a rabbit polyclonal antibody against GAA and was validated on Western blot.
Theoretical MW
98 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 3
using
NBP1-69295 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for LYAG/GAA Antibody

  • Acid alpha-Glucosidase
  • Acid Maltase
  • Aglucosidase alfa
  • EC 3.2.1.20
  • GAA
  • glucosidase, alpha; acid
  • LYAG
  • Lysosomal alphaGlucosidase
  • Lysosomal alpha-Glucosidase

Background

GAA is acid alpha-glucosidase, which is essential for the degradation of glycogen to glucose in lysosomes. Different forms of acid alpha-glucosidase are obtained by proteolytic processing. Defects in this gene are the cause of glycogen storage disease II, also known as Pompe's disease, which is an autosomal recessive disorder with a broad clinical spectrum. This gene encodes acid alpha-glucosidase, which is essential for the degradation of glycogen to glucose in lysosomes. Different forms of acid alpha-glucosidase are obtained by proteolytic processing. Defects in this gene are the cause of glycogen storage disease II, also known as Pompe's disease, which is an autosomal recessive disorder with a broad clinical spectrum. Three transcript variants encoding the same protein have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for LYAG/GAA Antibody (NBP1-69295) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: ICC.

Reviews using NBP1-69295:
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Images Ratings Applications Species Date Details
Immunocytochemistry LYAG/GAA NBP1-69295
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reviewed by:
Pierre Jean Beltran
ICC Human 04/05/2017
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Summary

ApplicationImmunocytochemistry
Sample TestedHuman fibroblast
SpeciesHuman
LotQC14285-42033

Comments

CommentsLYAG was probed in human fibroblasts fixed with 4% PFA. Cells were permeabilized and blocked in 0.1% Tween and 1% Bovine serum albumin. Anti-LYAG was incubated overnight at 4C in PBS with 0.1% Tween and 1% Bovine serum albumin. Secondary antibody was anti-rabbit conjugated to Alexa Fluor 568. LYAG is shown in green and the nucleus in blue (Hoescht stain).

Overall, the anitibody gave good signal, but with a significantly high cell-specific background as evident by some nuclear stain.

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Video Protocols

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Secondary Antibodies

 

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1
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Pierre Jean Beltran
04/05/2017
Application: ICC
Species: Human

Bioinformatics

Gene Symbol GAA
Entrez
Uniprot