Integrin alpha 2b/CD41 Antibody (745201) [Unconjugated]

Western blot shows lysates of human platelets. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Integrin alpha 2b/CD41 Monoclonal Antibody (Catalog # MAB7616) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Integrin alpha 2b/CD41 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.

Human peripheral blood platelets were stained with Fluorescein-conjugated Anti-Mouse Integrin alpha 2b/CD41 (Clone P2) and either (A) Mouse IgG1 Isotype Control (Catalog # MAB002) or (B) Mouse Anti-Human Integrin alpha 2b/CD41 Mono­clonal Antibody (Catalog # MAB7616) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). MAB7616 stains the same population as Clone P2.

Human peripheral blood platelets were stained with Mouse Anti-Human Integrin a2b/CD41 Mono-clonal Antibody (Catalog # MAB7616, filled histogram) or Mouse IgG1 Isotype Control (MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clone: 745201
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Integrin alpha 2b/CD41 Antibody (745201) [Unconjugated] Summary

• Immunogen: heterodimer of human Integrin alpha 2B (Leu32-Arg993; R887L) Accession P08514 + human Integrin beta 3 (Gly27-Asp718) Accession P05106
• Specificity: Detects human Integrin alpha 2b/CD41 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Integrin alpha 5, alpha 8, alpha V, beta 3, beta 5, beta 6, recombinant mouse Integrin beta 2b or beta 3 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGA2B
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha 2b/CD41 Antibody (745201) [Unconjugated]

• CD41 antigen
• CD41
• CD41BHPA3
• GP2B
• GP2Bintegrin alpha-IIb
• GPalpha IIb
• GPIIb
• GTA
• HPA3
• Integrin alpha 2b
• integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigenCD41)
• integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigenCD41B)
• ITGA2b
• ITGAB
• platelet fibrinogen receptor, alpha subunit
• Platelet membrane glycoprotein IIb
• platelet-specific antigen BAK

Background

Integrin alpha 2b (ITGA2b), also known as CD41 and GPIIb, is a transmembrane glycoprotein that is expressed by megakaryocytes and platelets. It is cleaved into two disulfide-linked chains (114 kDa and 22 kDa) during transit through the Golgi. Integrin alpha 2b associates with Integrin beta 3 to form complexes that interact with Fibrinogen, von Willebrand factor, Fibronectin, and Vitronectin. Integrin alpha 2b is required for platelet aggregation, and defects lead to disorders of coagulation. Within the extracellular domain, human Integrin alpha 2b shares 80% and 78% amino acid sequence identity with mouse and rat Integrin alpha 2b, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha 2b/CD41 Antibody (MAB7616)(5)

Publications using MAB7616

• H Odaka, K Hiemori, A Shimoda, K Akiyoshi, H Tateno CD63-positive extracellular vesicles are potential diagnostic biomarkers of pancreatic ductal adenocarcinoma BMC gastroenterology, 2022-03-28;22(1):153. 2022-03-28 [PMID: 35350978] (ELISA Detection, Human)
• WM Dickerson, R Yu, HU Westergren, J Paraskos, P Schatz, A Tigerstrom, A Ekman, J Sánchez, J Cheng, L Li, EY Chan Point-of-care microvolume cytometer measures platelet counts with high accuracy from capillary blood PLoS ONE, 2021-08-26;16(8):e0256423. 2021-08-26 [PMID: 34437590] (Interferent Testing, Human)
• H Odaka, K Hiemori, A Shimoda, K Akiyoshi, H Tateno Platelet-derived extracellular vesicles are increased in sera of Alzheimer's disease patients, as revealed by Tim4-based assays FEBS Open Bio, 2021-02-03;0(0):. 2021-02-03 [PMID: 33345458] (ELISA Detection, Human)
• S Giannini, MM Lee-Sundlo, L Rivadeneyr, CA Di Buduo, R Burns, JT Lau, H Falet, A Balduini, KM Hoffmeiste beta4GALT1 controls beta1 integrin function to govern thrombopoiesis and hematopoietic stem cell homeostasis Nat Commun, 2020;11(1):356. 2020 [PMID: 31953383] (Western Blot, Mouse)
• S Giannini, MM Lee-Sundlo, L Rivadeneyr, CA Di Buduo, R Burns, JT Lau, H Falet, A Balduini, KM Hoffmeiste beta4GALT1 controls beta1 integrin function to govern thrombopoiesis and hematopoietic stem cell homeostasis Nat Commun, 2020-01-17;11(1):356. 2020-01-17 [PMID: 31953383] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: ITGA2B