Ly-6G/Ly-6C Antibody (1A8) [Unconjugated]

MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B) and Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A).View our protocol for Staining Membrane-associated Proteins." > MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B) and Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A).View our protocol for Staining Membrane-associated Proteins." title="Mouse bone marrow cells were stained with either (A) Rat Anti-Mouse Ly-6g (GR-1) Monoclonal Antibody (Catalog # MAB10371) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B) and Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A).View our protocol for Staining Membrane-associated Proteins." />

Mouse bone marrow cells were stained with either (A) Rat Anti-Mouse Ly-6g (GR-1) Monoclonal Antibody (Catalog # MAB10371) or (B) Rat IgG2A Isotype Control (Catalog # <a class=

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 1A8
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Ly-6G/Ly-6C Antibody (1A8) [Unconjugated] Summary

• Immunogen: Ly-6G transfected EL-4J cell line
  Accession # P35461
• Specificity: Detects mouse Ly-6G in Flow Cytometry.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ly-6G/Ly-6C Antibody (1A8) [Unconjugated]

• GR
• Gr1
• Gr-1
• Ly-6
• Ly6G
• Ly-6G

Background

The myeloid differentiation antigen Gr-1, also known as Ly-6G, is a member of the Ly-6 family (1). The 1A8 antibody binds to Ly-6G while the RB6‑8C5 antibody also reacts weakly with Ly-6C-transfected EL4 cells (2). Ly-6G is a 21-25 kDa glycosyolphosphatidylinositol-anchored protein that is predominatly expressed in granulocytes in bone marrow.

1. Spangrude, G.J. et al. (1988) Science 241:58.
2. Fleming, T.J. et al. (1993) J. Immunol. 151:2399.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ly-6G/Ly-6C Antibody (MAB10371)(14)

Publications using MAB10371

• Greenman, R;Snir, T;Katav, A;Aricha, R;Mishalian, I;Hay, O;Frankel, M;Lawler, J;Saffioti, F;Pinzani, M;Thorburn, D;Peled, A;Mor, A;Vaknin, I; The Role of CCL24 in Primary Sclerosing Cholangitis: Bridging Patient Serum Proteomics to Preclinical Data Cells 2024-01-23 [PMID: 38334601] (Immunohistochemistry, Mouse)
• JH Riedel, L Robben, HJ Paust, Y Zhao, N Asada, N Song, A Peters, A Kaffke, AC Borchers, G Tiegs, L Seifert, NM Tomas, E Hoxha, UO Wenzel, TB Huber, T Wiech, JE Turner, CF Krebs, U Panzer Glucocorticoids target the CXCL9/10-CXCR3 axis and confer protection against immune-mediated kidney injury JCI Insight, 2023-01-10;0(0):. 2023-01-10 [PMID: 36355429] (Flow Cytometry, Mouse)
• A Kumar, PK Singh, Z Ahmed, S Singh, A Kumar Essential Role of NLRP3 Inflammasome in Mediating IL-1beta Production and the Pathobiology of Staphylococcus aureus Endophthalmitis Infection and Immunity, 2022-04-11;0(0):e0010322. 2022-04-11 [PMID: 35404106] (Flow Cytometry, In Vivo, Mouse, Transgenic Mouse)
• M Terasawa, K Hiramoto, R Uchida, K Suzuki Anti-Inflammatory Activity of Orally Administered Monostroma nitidum Rhamnan Sulfate against Lipopolysaccharide-Induced Damage to Mouse Organs and Vascular Endothelium Marine Drugs, 2022-02-03;20(2):. 2022-02-03 [PMID: 35200650] (IHC, Mouse)
• KHINM Herath, HJ Kim, A Kim, CE Sook, BY Lee, Y Jee The Role of Fucoidans Isolated from the Sporophylls of Undaria pinnatifida against Particulate-Matter-Induced Allergic Airway Inflammation: Evidence of the Attenuation of Oxidative Stress and Inflammatory Responses Molecules, 2020-06-22;25(12):. 2020-06-22 [PMID: 32580518] (IHC, Mouse)
• N Gupta, PK Singh, SG Revankar, PH Chandrasek, A Kumar Pathobiology of Aspergillus Fumigatus Endophthalmitis in Immunocompetent and Immunocompromised Mice Microorganisms, 2019-08-28;7(9):. 2019-08-28 [PMID: 31466325] (Neutrophil Depletion, Mouse)
• Kimmey J, Huynh J, Weiss L, Park S, Kambal A, Debnath J, Virgin H, Stallings C Unique role for ATG5 in neutrophil-mediated immunopathology during M. tuberculosis infection. Nature, 2015-12-09;528(7583):565-9. 2015-12-09 [PMID: 26649827] (Neutralization, Mouse)
• Yipp B, Kubes P Antibodies against neutrophil LY6G do not inhibit leukocyte recruitment in mice in vivo. Blood, 2013-01-03;121(1):241-2. 2013-01-03 [PMID: 23287627] (Neutralization, Mouse)
• Jarchum I, Liu M, Shi C, Equinda M, Pamer E Critical role for MyD88-mediated neutrophil recruitment during Clostridium difficile colitis. Infect Immun, 2012-06-11;80(9):2989-96. 2012-06-11 [PMID: 22689818] (IHC, Neutralization, Mouse)
• Tate M, Ioannidis L, Croker B, Brown L, Brooks A, Reading P The role of neutrophils during mild and severe influenza virus infections of mice. PLoS ONE, 2011-03-14;6(3):e17618. 2011-03-14 [PMID: 21423798] (Neutralization, Mouse)
• Wojtasiak M, Pickett D, Tate M, Londrigan S, Bedoui S, Brooks A, Reading P Depletion of Gr-1+, but not Ly6G+, immune cells exacerbates virus replication and disease in an intranasal model of herpes simplex virus type 1 infection. J Gen Virol, 2010-06-09;91(0):2158-66. 2010-06-09 [PMID: 20538903] (Flow Cytometry, Neutralization, Mouse)
• Dunay I, Fuchs A, Sibley L Inflammatory monocytes but not neutrophils are necessary to control infection with Toxoplasma gondii in mice. Infect Immun, 2010-02-09;78(4):1564-70. 2010-02-09 [PMID: 20145099] (Neutralization, Mouse)
• Tate M, Deng Y, Jones J, Anderson G, Brooks A, Reading P Neutrophils ameliorate lung injury and the development of severe disease during influenza infection. J Immunol, 2009-11-16;183(11):7441-50. 2009-11-16 [PMID: 19917678] (Neutralization, Mouse)
• Daley J, Thomay A, Connolly M, Reichner J, Albina J Use of Ly6G-specific monoclonal antibody to deplete neutrophils in mice. J Leukoc Biol, 2007-09-20;83(1):64-70. 2007-09-20 [PMID: 17884993] (Neutralization, Mouse)

Bioinformatics

• Uniprot:
  • P35461()