LXR alpha/NR1H3 Antibody

Western Blot: LXR alpha/NR1H3 Antibody [NBP2-17186] - Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE gel, diluted at 1:5000.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

LXR alpha/NR1H3 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human LXR alpha/NR1H3. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NR1H3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Reported in scientific literature (PMID:33850196)
  • Western Blot 1:1000-1:10000
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat (86%), Pig (85%), Rabbit (86%), Bovine (85%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for LXR alpha/NR1H3 Antibody

• alpha
• Liver X receptor alpha
• liver X receptor-alpha
• LXR alpha
• LXRA
• NR1H3
• Nuclear receptor subfamily 1 group H member 3
• nuclear receptor subfamily 1, group H, member 3
• oxysterols receptor LXR-alpha
• RLD-1

Background

The liver X receptors, LXRA and LXRB (NR1H2; MIM 600380), form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. The inducible LXRA is highly expressed in liver, adrenal gland, intestine, adipose tissue, macrophages, lung, and kidney, whereas LXRB is ubiquitously expressed. Ligand-activated LXRs form obligate heterodimers with retinoid X receptors (RXRs; see MIM 180245) and regulate expression of target genes containing LXR response elements (summary by Korf et al., 2009 [PubMed 19436111]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for LXR alpha/NR1H3 Antibody (NBP2-17186)(1)

Publication using NBP2-17186

• Slominski AT, Kim TK, Qayyum S et al. Vitamin D and lumisterol derivatives can act on liver X receptors (LXRs) Scientific reports 2021-04-13 [PMID: 33850196] (ICC/IF, Human)

FAQs for LXR alpha/NR1H3 Antibody (NBP2-17186). (Showing 1 - 1 of 1 FAQ).

1. What is band size for the antibody LXR alpha/NR1H3 Antibody?
   • The theoretical molecular weight of LXR alpha is ~50kDa. There are not many post translational modifications so the protein should run at about that size on a WB.https://www.uniprot.org/uniprot/Q13133#sequences

Bioinformatics

• Gene Symbol: NR1H3
• Uniprot:
  • Q13133(Human)