Western Blot: LXR alpha/NR1H3 Antibody [NB300-612] - LXRs are expressed in peripheral blood cells. LXR alpha/NR1H3 protein levels in protein extracts from PBMCs from these same donors were detected by Western blotting ...read more
Immunocytochemistry/ Immunofluorescence: LXR alpha/NR1H3 Antibody [NB300-612] - Analysis of LXR alpha (green) showing staining in the nucleus of Hela cells (right) compared to a negative control without primary antibody ...read more
Flow Cytometry: LXR alpha/NR1H3 Antibody [NB300-612] - analysis of LXR alpha was done on HeLa. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton X-100 for 20 minutes, and blocked with ...read more
Western Blot: LXR alpha/NR1H3 Antibody [NB300-612] - analysis was performed on nuclear enriched extracts (30 ug lysate) of MCF7 (Lane 1), THP-1 (Lane 2), A549 (Lane 3), SH-SY5Y (Lane 4), HL-60 (Lane 5) and KARPAS 299 ...read more
Immunocytochemistry/ Immunofluorescence: LXR alpha/NR1H3 Antibody [NB300-612] - Analysis of LXR alpha (green) showing staining in the nucleus of MCF-7 cells (right) compared to a negative control without primary ...read more
WB: Detects an approx. 64 kDa protein representing recombinant human LXR alpha. IHC usage was reported in the scientific literature (PMID: 17393442). Gel Shift Assays usage was reported in the scientific literature (PMID: 12470667).
Mouse reactivity reported in scientific literature (PMID: 17393442).
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
PBS with 1 mg/ml BSA
0.05% Sodium Azide
Immunogen affinity purified
Alternate Names for LXR alpha/NR1H3 Antibody
Liver X receptor alpha
liver X receptor-alpha
Nuclear receptor subfamily 1 group H member 3
nuclear receptor subfamily 1, group H, member 3
oxysterols receptor LXR-alpha
Liver X Receptors (LXRs) are oxysterol activated nuclear receptors which are involved in the regulation of cholesterol metabolism and bile acids. Two known isoforms, alpha and beta, are differentially expressed. The alpha isoform is predominantly expressed in liver, whereas the beta isoform expression has been shown to be ubiquitous.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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