Rat Lumican ELISA Kit (Colorimetric) Summary
| Specificity |
Natural and recombinant rat Lumican |
| Standard Curve Range |
156 - 10000 pg/mL (example only; lot dependent) |
| Sensitivity |
10 pg/mL (example only; lot dependent) |
| Assay Type |
Sandwich ELISA |
| Sample Volume |
50 - 100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
LUM |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Storage is content dependent. |
Kit Components
|
Components
|
- ABC Diluent Buffer, 1x12ml
- Antibody Diluent Buffer, 1x12ml
- Anti-rat Lumican (Biotin), 1x100ul (1:100 dilution)
- Avidin-Biotin-Peroxidase Complex (ABC), 1x100ul (1:100 dilution)
- Lumican Standard, 2x10ng
- Microtiter Strips, 1x96 wells
- Sample Diluent Buffer, 1x30ml
- TMB Color Developing Agent, 1x10ml
- TMB Stop Solution, 2N H2SO4, 1x10ml
- Wash Buffer, 25X, 1x20ml
|
Alternate Names for Rat Lumican ELISA Kit (Colorimetric)
Background
Lumican encodes a member of the small leucine-rich proteoglycan (SLRP) family that includes decorin, biglycan, fibromodulin, keratocan, epiphycan, and osteoglycin. In these bifunctional molecules, the protein moiety binds collagen fibrils and the highly charged hydrophilic glycosaminoglycans regulate interfibrillar spacings. Lumican is the major keratan sulfate proteoglycan of the cornea but is also distributed in interstitial collagenous matrices throughout the body. Lumican may regulate collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. [provided by RefSeq]
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for Lumican ELISA Kit (NBP3-20173). (Showing 1 - 1 of 1 FAQ).
-
What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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