LST1 Antibody (LST1/02)

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Western Blot: LST1 Antibody (LST1/02) [NBP1-45072] - Analysis (non-reducing conditions) of LST1 in whole cell lysate of U937 human Caucasian histiocytic lymphoma cell line. Lane 1: immunostaining with Isotype mouse ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IP
Clone
LST1/02
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

LST1 Antibody (LST1/02) Summary

Immunogen
KLH-fused peptide corresponding to amino acids SSEGPDLRGRDKRGT of human LST1
Specificity
The antibody LST1/02 reacts with an extracellular epitope of LST1, an approximately 6-11 kDa protein expressed as various transmembrane or soluble forms. LST1 is found predominantly on monocytes and dendritic cells. It migrates on SDS PAGE gels as approximately 25-28 kDa molecule.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LST1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:50
Application Notes
Western Blotting - It migrates on SDS PAGE gels as approximately 25-28 kDa molecule.
Theoretical MW
11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for LST1 Antibody (LST1/02)

  • B144D6S49ELST-1
  • leucocyte specific transcript 1
  • leukocyte specific transcript 1
  • leukocyte-specific transcript 1 protein
  • lymphocyte antigen 117
  • MGC119006
  • MGC119007
  • Protein B144

Background

LST1 (leukocyte-specific transcript, also known as B144) is expressed in cells of myeloid/erythroid lineage (monocytes, granulocytes, dendritic cells, platelets, erythrocytes). At least 14 alternatively spliced variants (LST1/A R11; LST1/N) can be detected; some of them (LST1/A, B, C, G, I, K) are transmembrane cell surface-exposed forms, the other are soluble. LST1 induces production of long, thin filopodia in dendritic cells, has an inhibitory effect on lymphocyte proliferation and may have an immunomodulatory role. LST1/A is an 11 kDa transmembrane adaptor present in membrane rafts and forms spontaneously covalent homodimers. Its intracellular domain contains two tyrosine motifs, one of them being an ITIM very similar to such motifs in Siglec.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LST1 Antibody (NBP1-45072) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-45072AF350
Alexa Fluor 405 NBP1-45072AF405
Alexa Fluor 488 NBP1-45072AF488
Alexa Fluor 532 NBP1-45072AF532
Alexa Fluor 594 NBP1-45072AF594
Alexa Fluor 647 NBP1-45072AF647
Alexa Fluor 700 NBP1-45072AF700
Alexa Fluor 750 NBP1-45072AF750
DyLight 350 NBP1-45072UV
DyLight 405 NBP1-45072V
DyLight 488 NBP1-45072G
DyLight 550 NBP1-45072R
DyLight 594 NBP1-45072DL594
DyLight 650 NBP1-45072C
DyLight 680 NBP1-45072FR
DyLight 755 NBP1-45072IR
Janelia Fluor 549 NBP1-45072JF549
Janelia Fluor 646 NBP1-45072JF646

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Bioinformatics

Gene Symbol LST1