LST1 Antibody (LST1/02) - BSA Free

Western Blot: LST1 Antibody (LST1/02) [NBP1-45072] - Analysis (non-reducing conditions) of LST1 in whole cell lysate of U937 human Caucasian histiocytic lymphoma cell line. Lane 1: immunostaining with Isotype mouse IgG1 control (PPV-06) Lane 2-3: immunostaining with anti-human LST1 (LST1/02), two independent batches. Note: It migrates on SDS PAGE gels as approximately 25-28 kDa molecule.

• Reactivity: Hu
• Applications: WB, ICC/IF, IP
• Clone: LST1/02
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

LST1 Antibody (LST1/02) - BSA Free Summary

• Immunogen: KLH-fused peptide corresponding to amino acids SSEGPDLRGRDKRGT of human LST1
• Specificity: The antibody LST1/02 reacts with an extracellular epitope of LST1, an approximately 6-11 kDa protein expressed as various transmembrane or soluble forms. LST1 is found predominantly on monocytes and dendritic cells. It migrates on SDS PAGE gels as approximately 25-28 kDa molecule.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: LST1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:50
  • Western Blot 1:1000
• Application Notes: Western Blotting - It migrates on SDS PAGE gels as approximately 25-28 kDa molecule.
• Theoretical MW: 11 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for LST1 Antibody (LST1/02) - BSA Free

• B144D6S49ELST-1
• leucocyte specific transcript 1
• leukocyte specific transcript 1
• leukocyte-specific transcript 1 protein
• lymphocyte antigen 117
• MGC119006
• MGC119007
• Protein B144

Background

LST1 (leukocyte-specific transcript, also known as B144) is expressed in cells of myeloid/erythroid lineage (monocytes, granulocytes, dendritic cells, platelets, erythrocytes). At least 14 alternatively spliced variants (LST1/A R11; LST1/N) can be detected; some of them (LST1/A, B, C, G, I, K) are transmembrane cell surface-exposed forms, the other are soluble. LST1 induces production of long, thin filopodia in dendritic cells, has an inhibitory effect on lymphocyte proliferation and may have an immunomodulatory role. LST1/A is an 11 kDa transmembrane adaptor present in membrane rafts and forms spontaneously covalent homodimers. Its intracellular domain contains two tyrosine motifs, one of them being an ITIM very similar to such motifs in Siglec.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LST1