LSD1 Antibody

Western Blot: LSD1 Antibody [NBP2-24629] - Analysis of LSD1 in human Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide and 3)mouse 3T3 cell lysate using NBP2-24629 at 2.0 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Simple Western: LSD1 Antibody [NBP2-24629] - Simple Western lane view shows a specific band for LSD1 in 0.5 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Product Discontinued

LSD1 Antibody Summary

• Immunogen: A portion of amino acids 450-500 of human LSD1 was used as the immunogen.
• Marker: Nucleus Marker
• Predicted Species: Rat (94%), Monkey (100%), Chimpanzee (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KDM1A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Simple Western 1:100
  • Western Blot 2.0-3.0 ug/ml
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.

Reactivity Notes

The amino acid sequence used as immunogen is 88% homologous in Chicken and 83% homologous in Zebrafish.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 ml PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for LSD1 Antibody

• amine oxidase (flavin containing) domain 2
• AOF2
• AOF2lysine-specific histone demethylase 1
• BHC110
• BHC110FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit
• Flavin-containing amine oxidase domain-containing protein 2
• KIAA0601KDM1
• LSD1
• LSD1BRAF35-HDAC complex protein BHC110
• lysine (K)-specific demethylase 1
• Lysine (K)specific Demethylase 1A
• Lysine (K)-specific Demethylase 1A
• lysine-specific histone demethylase 1A

Background

LSD1 a recently identified, first known lysine-specific histone demethylase, is an 886 amino acid nuclear protein belonging to flavin monoamine oxidase family. It contains a SWIRM domain, a FAD-binding motif and an amine oxidase domain. This protein is ubiquitously expressed and is a component of several histone deacetylase complexes. LSD1 acts as a component of the CoREST and other transcriptional co-repressor complexes and also plays an important role in silencing neuronal-specific genes in non-neuronal cells. It is also known to demethylate Lys-4 of histone H3, a specific tag for epigenetic transcriptional activation. Reports suggest that it plays an important role in stimulating androgen-receptor-dependent transcription converting oxygen to hydrogen peroxide (might use alternative electron acceptors). Along with nuclear FHL2 it serves as a novel biomarker predictive for prostate cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LSD1 Antibody (NBP2-24629)(1)

Publication using NBP2-24629

• Shen Z, Huang L, Jin S, Zheng Y Cloning and Expression Analysis of Two Kdm Lysine Demethylases in the Testes of Mature Yaks and Their Sterile Hybrids Animals (Basel) 2020-03-20 [PMID: 32244964] (WB, Bovine)
  
  Details:
  Maiwa yaks and cattle-yaks

Bioinformatics

• Gene Symbol: KDM1A
• Uniprot:
  • O60341(Human)