LSD1 Antibody

Images

 
Western Blot: LSD1 Antibody [NB100-537] - Detection of Human BHC110/LSD1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit ...read more
Immunohistochemistry: LSD1 Antibody [NB100-537] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-BHC110/LSD1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB staining using ...read more
Western Blot: LSD1 Antibody [NB100-537] - Nuclear extract (5 and 20 ug) from HeLa cells. Antibody used at 0.01 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

Order Details

LSD1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 825 and the C-terminus (residue 886) of human FAD-binding protein BRAF35-HDAC complex 110 kDa using the numbering given in TrEMBL entry O60341 (GeneID 23028).
Marker
Nucleus Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KDM1A
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:15000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Positive Control
HeLa Lysate (NB800-PC9)
LSD1 Lysate (NBL1-07573)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LSD1 Antibody

  • amine oxidase (flavin containing) domain 2
  • AOF2
  • AOF2lysine-specific histone demethylase 1
  • BHC110
  • BHC110FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit
  • Flavin-containing amine oxidase domain-containing protein 2
  • KIAA0601KDM1
  • LSD1
  • LSD1BRAF35-HDAC complex protein BHC110
  • lysine (K)-specific demethylase 1
  • Lysine (K)specific Demethylase 1A
  • Lysine (K)-specific Demethylase 1A
  • lysine-specific histone demethylase 1A

Background

This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for LSD1 Antibody (NB100-537)

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Bioinformatics

Gene Symbol KDM1A
Entrez
Uniprot