LRH-1/NR5A2 Antibody

Western Blot: LRH-1/NR5A2 Antibody [NBP2-42513] - Staining of Pig Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western Blot: LRH-1/NR5A2 Antibody [NBP2-42513] - Analysis of HepG2 nuclear lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Po, Mu, Rt, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

LRH-1/NR5A2 Antibody Summary

• Description: Novus Biologicals Goat LRH-1/NR5A2 Antibody (NBP2-42513) is a polyclonal antibody validated for use in WB and ELISA. Anti-LRH-1/NR5A2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-TDYDRSPFVTSPIS corresponding to internal region according to NP_003813.1.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_995582.1; NP_003813.1).
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NR5A2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.3-1 ug/ml
• Application Notes: WB: Approx. 55 kDa band observed in nuclear lysates of HeLa and HepG2 (calculated MW of 56.5 kDa band according to NP_003813.1).Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse Liver and NIH-3T3 cell line for this particular batch.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LRH-1/NR5A2 Antibody

• Alpha-1-fetoprotein transcription factor
• B1-binding factor
• b1-binding factor, hepatocyte transcription factor which activates enhancer IIof hepatitis B virus
• B1F
• B1F2
• fetoprotein-alpha 1 (AFP) transcription factor
• FTFLRH1
• FTZ-F1 beta
• FTZ-F1
• FTZ-F1beta
• HB1F
• hB1F-2
• hB1FCYP7A promoter-binding factor
• Hepatocytic transcription factor
• Liver receptor homolog 1
• liver receptor homolog-1
• LRH1
• LRH-1
• LRH-1CPF
• NR5A2
• nuclear receptor NR5A2
• nuclear receptor subfamily 5 group A member 2
• nuclear receptor subfamily 5, group A, member 2

Background

Neuregulin 1 (NRG1) was originally identified as a 44-kD glycoprotein that interacts with the NEU/ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. It is known that an extraordinary variety of different isoforms are produced from the NRG1 gene by alternative splicing. These isoforms include heregulins (HRGs), glial growth factors (GGFs) and sensory and motor neuron-derived factor (SMDF). They are tissue-specifically expressed and differ significantly in their structure. The HRG isoforms all contain immunoglobulin (Ig) and epidermal growth factor-like (EGF-like) domains. GGF and GGF2 isoforms contain a kringle-like sequence plus Ig and EGF-like domains; and the SMDF isoform shares only the EGF-like domain with other isoforms. The receptors for all NRG1 isoforms are the ERBB family of tyrosine kinase transmembrane receptors. Through interaction with ERBB receptors, NRG1 isoforms induce the growth and differentialtion of epithelial, neuronal, glial, and other types of cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LRH-1/NR5A2 Antibody (NBP2-42513)(1)

Publication using NBP2-42513

• Yumoto F, Nguyen P, Sablin EP et al. Structural basis of coactivation of liver receptor homolog-1 by beta-catenin. Proc Natl Acad Sci U S A 2012-01-03 [PMID: 22187462]

Bioinformatics

• Gene Symbol: NR5A2
• Entrez:
  • 2494(Human)
  • 26424(Mouse)
  • 60349(Rat)