LOX propeptide Antibodies
We offer LOX propeptide Antibodies for use in common research applications: Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot. Each LOX propeptide Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our LOX propeptide Antibodies can be used in a variety of model species: Human, Mouse, Porcine, Primate, Rat. Use the list below to choose the LOX propeptide Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our LOX propeptide polyclonal antibodies.
Alternate Names for LOX propeptide Antibodies
anti-LOX propeptide antibody, anti-LOX antibody, anti-lysyl oxidaseEC 1.4.3.13 antibody, anti-MGC105112 antibody, anti-protein-lysine 6-oxidase antibody
3 Results for "lox-propeptide" in Primary Antibodies
![Western Blot: LOX propeptide Antibody [NB110-41568] - Detection of LOPP in MC3T3-E1 cell lysate.](http://images.novusbio.com/fullsize/LOX-propeptide-Antibody-Western-Blot-NB110-41568-img0012.jpg "Western Blot: LOX propeptide Antibody [NB110-41568] - Detection of LOPP in MC3T3-E1 cell lysate.")
![Immunohistochemistry: LOX propeptide Antibody [NB110-41568] - LOX-PP was over-expressed in the vascular wall and in VSMC from TgLOX mice. (a) Representative immunohistochemical analysis showing LOX-PP staining (brown color) in aorta from both wild-type and TgLOX mice. The indicated areas are shown at high magnification (bars = 20 um). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-31312-w) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LOX-propeptide-Antibody-Immunohistochemistry-NB110-41568-img0017.jpg)
![Western Blot: LOX propeptide Antibody - BSA Free [NB110-41568] - LOX propeptide Antibody [NB110-41568] - Human VSMC were transduced with a lentiviral vector encoding for full-length LOX (pLOX; black bars), LOX-PP (pLOX-PP; grey bars) or with the corresponding empty vector (pLVX; V; white bars). (a) Immunoblots corresponding to mature LOX (mLOX; left panel) & LOX-PP (right panel). The position of the pro-enzyme (ProLOX, right panel), detected with the antibody against the propeptide. GAPDH was analyzed as a loading control. Displayed blots are not cropped from different gels or different parts of the same gel. Representative immunoblots from 3 independent experiments were shown. Image collected & cropped by Cite Ab from the following publication (//pubmed.ncbi.nlm.nih.gov/30185869/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LOX-propeptide-Antibody-Western-Blot-NB110-41568-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: LOX propeptide Antibody [NB110-41568] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-LOX propeptide at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/LOX-propeptide-Antibody-Immunocytochemistry-Immunofluorescence-NB110-41568-img0015.jpg)
![Western Blot: LOX propeptide Antibody [NB110-41568] - Total protein from human and mouse brain was separated on a 12% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/ml anti-LOX propeptide in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/LOX-propeptide-Antibody-Western-Blot-NB110-41568-img0014.jpg)
![Immunohistochemistry-Paraffin: LOX propeptide Antibody [NB110-41568] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse intestine using 1:200 dilution of rabbit anti- LOX propeptide (LOX-PP) antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This LOX-PP antibody generated a strong cytoplasmic staining in the epithelial and other cells with a majority of the signal localizing to luminal end of columner epithelial cells. There appears to be some nuclear positivity also which might be a nuclear variant of LOX-PP.](http://images.novusbio.com/fullsize/LOX-propeptide-Antibody-Immunohistochemistry-Paraffin-NB110-41568-img0011.jpg)
![Immunohistochemistry: LOX propeptide Antibody - BSA Free [NB110-41568] - Mature LOX & LOX-PP were over-expressed in the vascular wall & in VSMC from TgLOX mice. (a) Representative immunohistochemical analysis showing LOX & LOX-PP staining (brown color) in aorta from both wild-type & TgLOX mice. The indicated areas are shown at high magnification (bars = 20 µm). (b,c) Mature LOX & LOX-PP protein levels were determined by Western-blot in VSMC supernatants from these animals. The position of the pro-enzyme (ProLOX), mature LOX (mLOX) & LOX-PP forms are indicated. GAPDH was analyzed as loading control. Representative immunoblots from 3 independent experiments were shown. WT: wild-type; Tg: TgLOX. Displayed blots are not cropped from different gels or different parts of the same gel & images conform the digital image & integrity policies of the journal. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30185869), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-41568_rabbit-polyclonal-lox-propeptide-antibody-3102024162492.jpg)
![Western Blot: LOX propeptide Antibody - BSA Free [NB110-41568] - Over-expression of LOX-PP did not affect VSMC proliferation. Human VSMC were transduced with a lentiviral vector encoding for full-length LOX (pLOX; black bars), LOX-PP (pLOX-PP; grey bars) or with the corresponding empty vector (pLVX; V; white bars). (a) Immunoblots corresponding to mature LOX (mLOX; left panel) & LOX-PP (right panel) are shown. The position of the pro-enzyme (ProLOX, right panel), detected with the antibody against the propeptide, was also indicated. GAPDH was analyzed as a loading control. Displayed blots are not cropped from different gels or different parts of the same gel & images conform the digital image & integrity policies of the journal. Representative immunoblots from 3 independent experiments were shown. (b) Transduced VSMC were serum-starved for 24 h & then stimulated with 20% FCS. Cell proliferation was evaluated by the [3H]-thymidine incorporation method (left panel) or by cell count (right panel) in these cells. Results represented as mean ± SD. *P < 0.003 vs. pLVX (Kruskal-Wallis; at least n = 6). (c) [3H]-thymidine incorporation into DNA assessed in human VSMC transduced with lentiviral particles for full-length LOX (pLOX; black bars), or the corresponding empty vector (pLVX; white bars) treated or not with BAPN (500 µM). Results are represented as mean ± SD. P < 0.0001: * vs. pLVX; #vs. pLOX transduced cells (Two-way ANOVA; at least n = 9). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30185869), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-41568_rabbit-polyclonal-lox-propeptide-antibody-31020241624915.jpg)
![Western Blot: LOX propeptide Antibody [NBP1-30327] - LOX-PP reduces CIN85 mono-ubiquitination and ability to interact with c-Cbl. HEK293T cells were transfected with AMAP1-FLAG, CIN85, HA-c-Cbl, HA-ubiquitin and LOX-PP-GST (PP-GST) as indicated and subjected to a ubiquitination assay. FLAG-tagged AMAP1 was immunoprecipitated and total whole cell extracts were subjected to WB with the indicated antibodies (lower panel). Data were quantified and relative mono-ubiquitination of AMAP1 with and without LOX-PP was determined by averaging the results of three independent experiments. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0077288), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LOX-propeptide-Antibody-Western-Blot-NBP1-30327-img0010.jpg "Western Blot: LOX propeptide Antibody [NBP1-30327] - LOX-PP reduces CIN85 mono-ubiquitination and ability to interact with c-Cbl. HEK293T cells were transfected with AMAP1-FLAG, CIN85, HA-c-Cbl, HA-ubiquitin and LOX-PP-GST (PP-GST) as indicated and subjected to a ubiquitination assay. FLAG-tagged AMAP1 was immunoprecipitated and total whole cell extracts were subjected to WB with the indicated antibodies (lower panel). Data were quantified and relative mono-ubiquitination of AMAP1 with and without LOX-PP was determined by averaging the results of three independent experiments. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0077288), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: LOX propeptide Antibody [NBP1-30327] - Staining in Hela cells detected with a Dylight 488 labeled secondary antibody (Green) with Hoechst 33258 nuclear counterstain (Blue).](http://images.novusbio.com/fullsize/LOX-propeptide-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30327-img0003.jpg)
![Flow Cytometry: LOX propeptide Antibody [NBP1-30327] - An intracellular stain was performed on HeLa with LOX propeptide Antibody NBP1-30327 and a matched isotype control. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, .](http://images.novusbio.com/fullsize/LOX-propeptide-Antibody-Flow-Cytometry-NBP1-30327-img0009.jpg)
![Western Blot: LOX propeptide Antibody [NBP1-30327] - Analysis of LOX propeptide on MDA-MB-231 using NBP1-30327.](http://images.novusbio.com/fullsize/LOX-propeptide-Antibody-Western-Blot-NBP1-30327-img0006.jpg)
![Simple Western: LOX propeptide Antibody [NBP1-30327] - Simple Western lane view shows a specific band for LOX in 0.5 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/LOX-propeptide-Antibody-Simple-Western-NBP1-30327-img0008.jpg)