Loricrin Antibody

Immunohistochemistry: Loricrin Antibody [NBP1-33610] - Prominent differences in the expressions of innate immune and barrier molecules between sebaceous gland poor (SGR) and sebaceous gland rich (SGP) skin regions. Representative images for immunostaining and quantification of epidermal levels of LOR in SGP and SGR skin sections. Images of negative control stainings are shown in the bottom left corner of SGP immunostainings. Size bars=100um. The graphs show the mean +/- SEM of measured protein levels (*p<0.05; **p<0.01; ***p<0.001, as determined by Mann-Whitney U-test). Image collected and cropped by CiteAb from the following publication (frontiersin.org/articles/10.3389/fimmu.2018.00424/full), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: Loricrin Antibody [NBP1-33610] - Analysis of methanol-fixed Hep3B, using antibody at 1:500 dilution.

Western Blot: Loricrin Antibody [NBP1-33610] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Loricrin antibody [N1], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: Loricrin Antibody [NBP1-33610] - HBL438 xenograft. Loricrin antibody [N1], N-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: Loricrin Antibody [NBP1-33610] - Reconstructed human epidermis tissues were embedded in paraffin. After successives baths in xylene and ethanol, antigen was retrieved using warm citrate (for 30 min). Specimens were incubated overnight with the primary antibody (1:100 dilution). Blue staining corresponds to nuclei while loricrin is visible in green. IHC-P image submitted by a verified customer review.

Simple Western: Loricrin Antibody [NBP1-33610] - NHEK human normal epidermal keratinocytes. Antibody dilution of 1:50. Protein concentration of 450 ug/mL. Detection is chemiluminescence. Simple Western image submitted by a verified customer review.

Western Blot: Loricrin Antibody [NBP1-33610] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Loricrin antibody [N1], N-term (NBP1-33610) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, RM
• Applications: WB, Simple Western, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Loricrin Antibody Summary

• Description: Novus Biologicals Rabbit Loricrin Antibody (NBP1-33610) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and Simple Western. Anti-Loricrin Antibody: Cited in 9 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Loricrin. The exact sequence is proprietary.
• Localization: Cytoplasm, Nucleus, nucleoplasm
• Predicted Species: Rhesus Macaque (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LORICRIN
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Simple Western 1:50
  • Western Blot 1:500-1:3000
• Application Notes: See Simple Western Antibody Database for Simple Western validation: Tested in NHEK human normal epidermal keratinocytes, separated by Size, antibody dilution of 1:50
• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (87%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Loricrin Antibody

• loricrin
• LRN
• MGC111513

Background

This gene encodes loricrin, a major protein component of the cornified cell envelope found in terminally differentiated epidermal cells. Mutations in this gene are associated with Vohwinkel's syndrome and progressive symmetric erythrokeratoderma, both inherited skin diseases. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Loricrin Antibody (NBP1-33610)(10)

Publications using NBP1-33610

• Shigehara Y, Okuda S, Nemer G et al. Mutations in SDR9C7 gene encoding an enzyme for vitamin A metabolism underlie autosomal recessive congenital ichthyosis. Hum. Mol. Genet. 2016-08-18 [PMID: 27538420] (ICC/IF, Human)
• Chung S, Kim M, Choi S et al. The advanced 3D lymphatic system for assaying the human cutaneous lymphangiogenesis in the microfluidic platform Research Square 2023-05-04 (Immunocytochemistry/ Immunofluorescence, Human)
• Kim, HS;Kim, HJ;Hong, YD;Son, ED;Cho, SY; ?-endorphin suppresses ultraviolet B irradiation-induced epidermal barrier damage by regulating inflammation-dependent mTORC1 signaling Scientific reports 2023-12-15 [PMID: 38102220]
• Somogyi O, Dajnoki Z, Szab� L et al. New Data on the Features of Skin Barrier in Hidradenitis Suppurativa Biomedicines 2023-01-04 [PMID: 36672635] (Immunocytochemistry/ Immunofluorescence, Human)
• Kim HM, Kang YM, Jin BR et al. Morus alba fruits Attenuates Atopic Dermatitis Symptoms and Pathology in vivo and in vitro via the Regulation of Barrier Function, immune response and pruritus Phytomedicine 2022-11-01 [PMID: 36610150] (WB, Mouse)
• Gil Ty, Jin BR, An HJ Peucedanum japonicum Thunberg alleviates atopic dermatitis-like inflammation via STAT/MAPK signaling pathways in vivo and in vitro Molecular immunology 2022-02-23 [PMID: 35219015]
• Lee J, Song K, Jung C Diosmin restores the skin barrier by targeting the aryl hydrocarbon receptor in atopic dermatitis Phytomedicine 2020-11-01 [PMID: 33302042] (WB, Human)
  
  Details:
  Human epidermal keratinocytes were analyzed by Western blot.
• Medgyesi B, Dajnoki Z, et al. Rosacea Is Characterized by a Profoundly Diminished Skin Barrier. J Invest Dermatol 2020-10-01 [PMID: 32199994] (IF/IHC, Human)
• Beke G, Dajnoki Z, Kapitany A et al. Immunotopographical Differences of Human Skin. Front Immunol. 2018-03-05 [PMID: 29556238] (IHC-P, Human)
• Shigehara Y, Okuda S, Nemer G et Al. Mutations in SDR9C7 gene encoding an enzyme for vitamin A metabolism underlie autosomal recessive congenital ichthyosis Hum Mol Genet 2018-02-26 [PMID: 28173123]

Reviews for Loricrin Antibody (NBP1-33610) (3)

Average Rating: 4.3

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Human.

reviewed by: Agnès Tessier IHC-P Human 02/27/2020

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Skin tissue
• Species: Human
• Lot: 42753

Comments

• Comments: Reconstructed human epidermis tissues were embedded in paraffin. After successives baths in xylene and ethanol, antigen was retrieved using warm citrate (for 30 min). After a step of saturation, spécimens were incubated overnight with the primary antibody (1:100 dilution). Blue staining corresponds to nuclei while loricrin is visible in green.

reviewed by: Irini Dijkhoff Simple Western Human 05/24/2019

Summary

• Application: Simple Western
• Sample Tested: NHEK human normal epidermal keratinocytes
• Species: Human
• Lot: 42753

Comments

• Comments: Standard JESS run default settings.

reviewed by: David Krull IHC-P Human 12/08/2016

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: human skin
• Species: Human

Bioinformatics

• Gene Symbol: LORICRIN