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LMO2 Antibody

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Immunohistochemistry-Paraffin: LMO2 Antibody [NB110-83978] - IHC analysis of a formalin fixed and paraffin embedded tissue section of human tonsil using LMO2 antibody NB110-83978 at 1:500 dilution. HRP-labeled secondary antibody and DAB reagent were used for the detection of LMO2 signal and the sections were further counterstained with hematoxylin. This LMO2 antibody generated an expected nuclear staining in a subset of cells in the germinal centers of tonsil section.
Immunohistochemistry-Paraffin: LMO2 Antibody [NB110-83978] - IHC analysis of a formalin fixed and paraffin embedded tissue section of human tonsil using LMO2 antibody NB110-83978 at 1:500 dilution. HRP-labeled secondary ...read more
Western Blot: LMO2 Antibody [NB110-83978] - Detection of LMO2 in Ramos whole cell lysate using NB110-83978.
Immunohistochemistry-Paraffin: LMO2 Antibody [NB110-83978] - IHC analysis of a formalin fixed and paraffin embedded tissue section of human tonsil using LMO2 antibody NB110-83978 at 1:500 dilution. HRP-labeled secondary ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, ZeSpecies Glossary
Applications WB, ChIP, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB110-83978AF350
Alexa Fluor 405
NB110-83978AF405
Alexa Fluor 488
NB110-83978AF488
Alexa Fluor 532
NB110-83978AF532
Alexa Fluor 594
NB110-83978AF594
Alexa Fluor 647
NB110-83978AF647
Alexa Fluor 700
NB110-83978AF700
Alexa Fluor 750
NB110-83978AF750
Biotin
NB110-83978B
DyLight 350
NB110-83978UV
DyLight 405
NB110-83978V
DyLight 488
NB110-83978G
DyLight 550
NB110-83978R
DyLight 594
NB110-83978DL594
DyLight 650
NB110-83978C
DyLight 680
NB110-83978FR
DyLight 755
NB110-83978IR
HRP
NB110-83978H
Janelia Fluor 549
NB110-83978JF549
Janelia Fluor 646
NB110-83978JF646
100% Guarantee on every product

LMO2 Antibody Summary

Immunogen
Synthetic peptide made to an N-terminal portion of the human LMO2 protein (within residues 1-100). [Swiss-Prot# P25791]
Localization
Nuclear (potential)
Predicted Species
Zebrafish (93%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LMO2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/ml
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunohistochemistry 1:250 - 1:500
  • Immunohistochemistry-Paraffin 1:250 - 1:500
Application Notes
This LMO2 antibody is useful for ChIP and Western blot, where a band is seen at ~24 kDa. In WB, non-specific staining can be seen at higher molecular weights. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LMO2 Antibody

  • Cysteine-rich protein TTG-2
  • LIM domain only 2 (rhombotin-like 1)
  • LIM domain only protein 2
  • LMO2
  • LMO-2
  • RBTN2
  • RBTN2rhombotin-2
  • RBTNL1
  • RBTNL1rhombotin-like 1
  • RHOM2
  • RHOM2T-cell translocation protein 2
  • T-cell translocation gene 2
  • TTG2
  • TTG2rhombotin 2

Background

LMO2 (LIM-only protein 2) is a nuclear transcriptional regulator essential for normal blood cell development and it exerts its effects by mediating protein-protein interactions as well as by nucleating multicomponent transcriptional complexes. LMO2 interacts with LDB1 (LIM domain binding protein 1) through the tandem LIM domains of LMO2 and LID (LIM interaction domain) of LDB1. LMO2 also interacts with bHlH proteins TAL1/SCL, BEX2 and KDM5A, and form complex with TAL1/SCL. It functions with TAL1/SCL in the regulation of RBCs development and with LDB1 to maintain erythroid precursors in immature state. LMO2 has been associated with the maintenance of hematopoietic stem cells, RBCs differentiation, and angiogenesis in both normal development as well as during carcinogenesis. Disruption of LMO2 in mice causes death in midway through embryonic development as they do not develop RBCs. Excessive expression of LMO2 in T-cells, through either chromosomal translocations or retroviral insertion of gene therapy vectors, as well as transgenic overexpression of LMO2 in mouse model leads to the onset of T-cell acute lymphoblastic leukemia (T-ALL).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: SCL/Tal1 Antibody (5H1) [NBP2-01488] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Tal1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Tal1.
Immunocytochemistry/Immunofluorescence: SCL/Tal1 Antibody (5H1) [NBP2-01488] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Tal1.
Immunohistochemistry-Paraffin: SCL/Tal1 Antibody (5H1) [NBP2-01488] - Staining of paraffin-embedded Human prostate tissue using anti-Tal1 mouse monoclonal antibody.
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
GATA-1 Antibody
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Western Blot: GATA-1 Antibody (4F5) [NBP1-47492] - Analysis using GATA1 mouse mAb against K562 (1) cell lysate.
Immunocytochemistry/Immunofluorescence: GATA-1 Antibody (4F5) [NBP1-47492] - Analysis of K562(left) cells using GATA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry: GATA-1 Antibody (4F5) [NBP1-47492] - Staining of Fetal Baboon Liver. Antigen retrieval: 10mM sodium citrate, pH 6.0, Primary Ab: 1:500 dilution, Secondary Ab (biotinylated): 1:200, Streptavidin-AlexaFluor488: 1:500 (green), Quench autofluorescence with 1% Sudan Black in 70% methanol. Image from verified customer review.
(1 Review)
(1 Publication)
Species: Hu, Bb
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
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Western Blot: SCLY Antibody [NBP2-48693] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MGLane 4: Human plasmaLane 5: Human Liver tissue
Immunocytochemistry/Immunofluorescence: SCLY Antibody [NBP2-48693] - Immunofluorescent staining of human cell line HeLa shows localization to the Golgi apparatus.
Immunohistochemistry-Paraffin: SCLY Antibody [NBP2-48693] - Staining of human liver shows strong nuclear positivity in hepatocytes.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
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Western Blot: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Western Blot Analysis of human HeLa cell lysate using Cytokeratin 7 Antibody (OV-TL12/30).
Immunocytochemistry/Immunofluorescence: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Immunofluorescent staining of methanol-fixed HeLa cells. Cytokeratin 7 Antibody (OV-TL12/30) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Paraffin-embedded human salivary gland tissue. Image from verified customer review. Image using the Azide and BSA Free format of this antibody.
(1 Review)
(3 Publications)
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Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
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NBP1-31362
Western Blot: LDB1 Antibody [NBP1-31362] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LDB1 antibody diluted by 1:1000.
Immunocytochemistry/Immunofluorescence: LDB1 Antibody [NBP1-31362] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LDB1 protein stained by LDB1 antibody [N2C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: LDB1 Antibody [NBP1-31362] - mouse muscle.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
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Immunocytochemistry/Immunofluorescence: LMO1 Antibody (4F7) [H00004004-M01] - Analysis of monoclonal antibody to LMO1 on HeLa cell. Antibody concentration 10 ug/ml.
Species: Hu
Applications: WB, ELISA, ICC/IF
LDB2 Antibody
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Western Blot: LDB2 Antibody [NB600-480] - Analysis of a 43-kDa band corresponding to LDB2 in a lysates prepared from human kidney (lane 1) and mouse spleen (lane 2) tissues. Approximately 18 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-LDB2 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.
Immunohistochemistry: LDB2 Antibody [NB600-480] - Used at a 5 ug/ml to detect LDB2 in human brain cortex tissue. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain. Tissue was formalin-fixed and paraffin embedded.
Species: Hu, Mu, Rt, Ca, Ch, Cz
Applications: WB, ELISA, IHC, IHC-P
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(826927)
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Western blot shows lysates of D3 mouse embryonic stem cell line and P19 mouse embryonal carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse TCF‑3/E2A Monoclonal Antibody (Catalog # MAB7650) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for TCF‑3/E2A at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Western blot shows lysates of LNCaP human prostate cancer cell line and SH‑SY5Y human neuroblastoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse GATA‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2046) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GATA‑2 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Immunocytochemistry/Immunofluorescence: PDLIM5 Antibody [NBP1-84843] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane, cytosol & focal adhesion sites.
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(1 Publication)
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Flow Cytometry: PU.1/Spi-1 Antibody [NBP2-27163] - PU.1/Spi1 Antibody [NBP2-27163] - Intracellular analysis of PU.1 in mouse RAW cells (top) and PMA treated (20 ng/ml, overnight) human ThP1 cells (bottom) using 1.25 ug/10^6 cells. Green peak represents isotype control this antibody ; red represents anti-PU.1 antibody.
Western Blot: PU.1/Spi1 Antibody [NBP2-27163] - analysis of 293T cell lysate (30ug) using anti-PU.1/Spi1 antibody. Image from verified customer review.
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Western Blot: HOX11 Antibody [NB100-56424] - Annalysis of HOX11 in A) human, B) mouse and C) rat liver cell lysate using NB100-56424 at 1 ug/ml.
Species: Hu, Mu, Rt
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GATA-2 Antibody
[Unconjugated]
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Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line and KG‑1 human acute myelogenous leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse GATA‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2046) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GATA‑2 at approximately 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of KG‑1 human acute myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for GATA‑2 at approximately 64 kDa (as indicated) using 5 µg/mL of Goat Anti-Human GATA‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2046) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
Western blot shows lysates of LNCaP human prostate cancer cell line and SH‑SY5Y human neuroblastoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse GATA‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2046) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GATA‑2 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Applications: WB, Simple Western, IHC, ICC
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LMO4 Antibody
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Applications: WB
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Western Blot: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779] - Analysis of RUNX1+RUNX2+RUNX3 on Jurkat cells lysates using anti-RUNX1+RUNX2+RUNX3 antibody at 1/1,000 dilution.
Immunocytochemistry/Immunofluorescence: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779] - Staining RUNX1+RUNX2+RUNX3 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: RUNX1/CBFA2 Antibody (SD0803) [NBP2-67779] - Analysis of paraffin-embedded mouse testis tissue using anti-RUNX1+RUNX2+RUNX3 antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
PDLIM4 Antibody
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Western Blot: PDLIM4 Antibody [NBP1-80833] - Analysis in human cell line RH-30.
Immunocytochemistry/Immunofluorescence: PDLIM4 Antibody [NBP1-80833] - Staining of human cell line U-2 OS shows localization to actin filaments. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PDLIM4 Antibody [NBP1-80833] - Staining of human small intestine shows weak to moderate cytoplasmic positivity in glandular cells.
(3 Publications)
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
PDLIM7 Antibody
NBP1-84841
Western Blot: PDLIM7 Antibody [NBP1-84841] - Analysis using Anti-PDLIM7 antibody NBP1-84841 (A) shows similar pattern to independent antibody NBP2-58734 (B).
Immunocytochemistry/Immunofluorescence: PDLIM7 Antibody [NBP1-84841] - Staining of human cell line U-251 MG shows localization to actin filaments and focal adhesion sites. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PDLIM7 Antibody [NBP1-84841] - Staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.
(2 Publications)
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
CSRP3 Antibody
NBP1-06983
Western Blot: CSRP3 Antibody [NBP1-06983] - (0.01ug/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunohistochemistry-Paraffin: CSRP3 Antibody [NBP1-06983] - staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: CSRP3 Antibody [NBP1-06983] - Staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
(2 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP, PEP-ELISA
LMO2 Antibody
NB110-83978
LMO2 Antibody
Species: Hu, Mu, Rt, Bv, Ze
Applications: WB, ChIP, IHC, IHC-P

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Product General Protocols

View specific protocols for LMO2 Antibody (NB110-83978): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol

FAQs for LMO2 Antibody (NB110-83978) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB110-83978AF350
Alexa Fluor 405 NB110-83978AF405
Alexa Fluor 488 NB110-83978AF488
Alexa Fluor 532 NB110-83978AF532
Alexa Fluor 594 NB110-83978AF594
Alexa Fluor 647 NB110-83978AF647
Alexa Fluor 700 NB110-83978AF700
Alexa Fluor 750 NB110-83978AF750
Biotin NB110-83978B
DyLight 350 NB110-83978UV
DyLight 405 NB110-83978V
DyLight 488 NB110-83978G
DyLight 550 NB110-83978R
DyLight 594 NB110-83978DL594
DyLight 650 NB110-83978C
DyLight 680 NB110-83978FR
DyLight 755 NB110-83978IR
HRP NB110-83978H
Janelia Fluor 549 NB110-83978JF549
Janelia Fluor 646 NB110-83978JF646
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol LMO2
Entrez
Uniprot
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