Cytokeratin 7 Antibody (OV-TL12/30)

Images

 
Western Blot: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Western Blot Analysis of human HeLa cell lysate using Cytokeratin 7 Antibody (OV-TL12/30).
Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Immunofluorescent staining of methanol-fixed HeLa cells. Cytokeratin 7 Antibody (OV-TL12/30) followed by goat anti-Mouse ...read more
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Paraffin-embedded human salivary gland tissue. Image from verified customer review. Image using the Azide and BSA Free format of this ...read more
Flow Cytometry: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Flow Cytometric Analysis of HeLa cells using Cytokeratin 7 Antibody (OV-TL12/30) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Human Lung SCC stained with Cytokeratin 7 Monoclonal Antibody (OV-TL12/30)
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Human Ovarian Carcinoma stained with Cytokeratin 7 Monoclonal Antibody (OV-TL12/30)
Simple Western: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Simple Western lane view shows a specific band for Cytokeratin 7 in 0.1 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions ...read more
Simple Western: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 7 antibody was used at 1 ug/ml dilution of HeLa lysates(s) respectively.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
Clone
OV-TL12/30
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Cytokeratin 7 Antibody (OV-TL12/30) Summary

Immunogen
OTN 11, ovarian carcinoma cell line
Localization
Cytoplasmic
Marker
Glandular and Transitional Epithelial Marker
Specificity
It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
KRT7
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Simple Western 1.0 ug/ml
  • Flow Cytometry 5 - 10 ul/million cells in 0.1ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:200
Application Notes
Hu-chromosome location: 12q13.13
Immunohistochemistry-Paraffin 1:100 - 1:200 for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 30239759). Use in Immunohistochemistry reported in scientific literature (PMID: 30522949). The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Cytokeratin 7 Knockout HeLa Cell Lysate
Publications
Read Publications using
NBP2-44814 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30522949).

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Cytokeratin 7 Antibody (OV-TL12/30)

  • CK7
  • CK-7
  • cytokeratin 7
  • cytokeratin-7
  • K2C7
  • K7keratin, 55K type II cytoskeletal
  • keratin 7
  • keratin, type II cytoskeletal 7
  • keratin-7
  • MGC129731
  • MGC3625
  • sarcolectin
  • SCLkeratin, simple epithelial type I, K7
  • type II mesothelial keratin K7
  • Type-II keratin Kb7

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-67309
Species: Hu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NB100-687
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
NB300-223
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Eq
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NB120-22711
Species: Hu, Mu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-85630
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-01488
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-44501
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, IF
NBP2-16094
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, KO
NBP2-67717
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-34594
Species: Hu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready
AF4478
Species: Hu
Applications: WB, IHC
H00005670-Q01
Species: Hu
NBP2-34270
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP2-38740
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-47492
Species: Hu, Pm
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NB200-103
Species: Hu, Mu, Rt, Ch, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NBP2-16382
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-22194
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
NBP2-44814
Species: Hu, Mu
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P

Publications for Cytokeratin 7 Antibody (NBP2-44814)(3)

Reviews for Cytokeratin 7 Antibody (NBP2-44814) (0)

There are no reviews for Cytokeratin 7 Antibody (NBP2-44814). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cytokeratin 7 Antibody (NBP2-44814) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47940AF350
Alexa Fluor 405 NBP2-47940AF405
Alexa Fluor 488 NBP2-47940AF488
Alexa Fluor 532 NBP2-47940AF532
Alexa Fluor 594 NBP2-47940AF594
Alexa Fluor 647 NBP2-47940AF647
Alexa Fluor 700 NBP2-47940AF700
Alexa Fluor 750 NBP2-47940AF750
Allophycocyanin NBP2-47940APC
Biotin NBP2-47940B
DyLight 350 NBP2-47940UV
DyLight 405 NBP2-47940V
DyLight 488 NBP2-47940G
DyLight 550 NBP2-47940R
DyLight 594 NBP2-47940DL594
DyLight 650 NBP2-47940C
DyLight 680 NBP2-47940FR
DyLight 755 NBP2-47940IR
FITC NBP2-47940F
HRP NBP2-47940H
Janelia Fluor 549 NBP2-47940JF549
Janelia Fluor 646 NBP2-47940JF646
PE NBP2-47940PE
PerCP NBP2-47940PCP

Additional Cytokeratin 7 Products

Bioinformatics Tool for Cytokeratin 7 Antibody (NBP2-44814)

Discover related pathways, diseases and genes to Cytokeratin 7 Antibody (NBP2-44814). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Cytokeratin 7 Antibody (NBP2-44814)

Discover more about diseases related to Cytokeratin 7 Antibody (NBP2-44814).
 

Pathways for Cytokeratin 7 Antibody (NBP2-44814)

View related products by pathway.

PTMs for Cytokeratin 7 Antibody (NBP2-44814)

Learn more about PTMs related to Cytokeratin 7 Antibody (NBP2-44814).
 

Research Areas for Cytokeratin 7 Antibody (NBP2-44814)

Find related products by research area.

Blogs on Cytokeratin 7

There are no specific blogs for Cytokeratin 7, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Cytokeratin 7 Antibody (OV-TL12/30) and receive a gift card or discount.

Bioinformatics

Gene Symbol KRT7
Uniprot
COVID-19 update