Cytokeratin 7 Antibody (OV-TL12/30)

Western Blot: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Western Blot Analysis of human HeLa cell lysate using Cytokeratin 7 Antibody (OV-TL12/30).

Immunocytochemistry/Immunofluorescence: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Immunofluorescent staining of methanol-fixed HeLa cells. Cytokeratin 7 Antibody (OV-TL12/30) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Paraffin-embedded human salivary gland tissue. Image from verified customer review. Image using the Azide and BSA Free format of this antibody.

Flow Cytometry: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Flow Cytometric Analysis of HeLa cells using Cytokeratin 7 Antibody (OV-TL12/30) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Human Lung SCC stained with Cytokeratin 7 Monoclonal Antibody (OV-TL12/30)

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Human Ovarian Carcinoma stained with Cytokeratin 7 Monoclonal Antibody (OV-TL12/30)

Simple Western: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Simple Western lane view shows a specific band for Cytokeratin 7 in 0.1 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 7 antibody was used at 1 ug/ml dilution of HeLa lysates(s) respectively.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
• Clone: OV-TL12/30
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Cytokeratin 7 Antibody (OV-TL12/30) Summary

• Immunogen: OTN 11, ovarian carcinoma cell line
• Localization: Cytoplasmic
• Marker: Glandular and Transitional Epithelial Marker
• Specificity: It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT7
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot
  • Simple Western 1.0 ug/ml
  • Flow Cytometry 5 - 10 ul/million cells in 0.1ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:200
• Application Notes: Hu-chromosome location: 12q13.13
  Immunohistochemistry-Paraffin 1:100 - 1:200 for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
  Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 30239759). Use in Immunohistochemistry reported in scientific literature (PMID: 30522949). The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Cytokeratin 7 Knockout HeLa Cell Lysate

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30522949).

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Cytokeratin 7 Antibody (OV-TL12/30)

• CK7
• CK-7
• cytokeratin 7
• cytokeratin-7
• K2C7
• K7keratin, 55K type II cytoskeletal
• keratin 7
• keratin, type II cytoskeletal 7
• keratin-7
• MGC129731
• MGC3625
• sarcolectin
• SCLkeratin, simple epithelial type I, K7
• type II mesothelial keratin K7
• Type-II keratin Kb7

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cytokeratin 7 Antibody (NBP2-44814)(3)

Publications using NBP2-44814

• Steele N, Chakrabarti J, Wang J et al. An Organoid-Based Preclinical Model of Human Gastric Cancer Cellular and Molecular Gastroenterology and Hepatology Sep 2 2018 [PMID: 30522949] (IHC, Mouse)
• Mani S, Ghosh J, Lan Y et al. Epigenetic changes in reterm birth placenta suggest a role for ADAMTS genes in spontaneous preterm birth. Hum. Mol. Genet. Sep 18 2018 [PMID: 30239759] (ICC/IF, Human)
• Kallol S, Huang X, Muller S et al. Novel Insights into Concepts and Directionality of Maternal Fetal Cholesterol Transfer across the Human Placenta. Int J Mol Sci. 2018 Aug 9 [PMID: 30096856] (Flow, Human)
  
  Details:
  Citation using the Alexa Fluor 488 form of this antibody.

Bioinformatics

• Gene Symbol: KRT7
• Uniprot:
  • P08729(Human)