LDB1 Antibody

Images

 
Genetic Strategies: Knockout Validated: LDB1 Antibody [NBP1-31362] - Wild-type (WT) and LDB1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LDB1 ...read more
Western Blot: LDB1 Antibody [NBP1-31362] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with LDB1 antibody diluted by 1:1000.
Immunocytochemistry/ Immunofluorescence: LDB1 Antibody [NBP1-31362] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LDB1 protein stained by LDB1 antibody [N2C3] diluted at 1:500. Blue: Hoechst ...read more
Immunohistochemistry-Paraffin: LDB1 Antibody [NBP1-31362] - mouse muscle.
Western Blot: LDB1 Antibody [NBP1-31362] - Sample: 1000 ug 293T whole cell lysate/extract A. 20 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgGC. Immunoprecipitation of LDB1 protein by ...read more
Western Blot: LDB1 Antibody [NBP1-31362] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: LDB1 Antibody [NBP1-31362] - Paraffin-embedded U87 Xenograft, using antibody at 1:100 dilution.
Immunohistochemistry-Paraffin: LDB1 Antibody [NBP1-31362] - rat middle brain.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ch, Pm, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IP, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

LDB1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human LDB1. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Porcine (98%), Chicken (97%), Xenopus (97%), Monkey (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LDB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Knockout Validated
  • Western Blot 1:500-1:3000
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Brain Cerebellum Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Rhesus Monkey (100%), Xenopus laevis (97%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for LDB1 Antibody

  • carboxy terminal LIM domain protein 2
  • Carboxyl-terminal LIM domain-binding protein 2
  • CLIM-2
  • CLIM2hLdb1
  • LDB-1
  • LIM domain binding 1
  • LIM domain-binding factor-1
  • LIM domain-binding protein 1
  • NLILIM domain-binding factor CLIM2
  • Nuclear LIM interactor

Background

LDB1 binds to the LIM domain of a wide variety of LIM domain-containing transcription factors. May regulate the transcriptional activity of LIM-containing proteins by determining specific partner interactions. May play a role in the development of motor neuron

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol LDB1