Livin Antibody

Images

 
Western Blot: Livin Antibody [NB100-56145] - Analysis of full-length, recombinant human Livin using this antibody.
Immunohistochemistry-Paraffin: Livin Antibody [NB100-56145] - Analysis of Livin in Bouin's-fixed, paraffin-embedded E13.5 mouse embryo (13.5 days post coitum) Bouin's using this antibody at 1:2000. The mother was ...read more
Immunohistochemistry: Livin Antibody [NB100-56145] - Immunohistochemical analysis of Livin in formalin-fixed, paraffin embedded human tonsil (secondary nodule) using this antibody at 1:2000. Hematoxylin-eosin ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Livin Antibody Summary

Immunogen
A synthetic peptide corresponding to amino acids 180-230 of human Livin, isoform alpha and isoform beta was used as immunogen (NP_647478).
Specificity
Livin is a short-lived protein that is thought to be degraded by the ubiquitin-proteasome pathway. Thus users may want to do a time course when examining the effects of treatments on Livin expression. Users are encouraged to refer to the literature for additional information; for example, Ma et al. 2006. 2. Livin has been shown to undergo proteolytic cleavage during apoptosis. Thus the molecular weight of Livin observed on Western blots may vary depending on the presence of absence of cleavage fragments.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BIRC7
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-56145 in the following applications:

  • 3 publications
  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Neat whole antisera
Preservative
0.05% Sodium Azide
Purity
Unpurified

Alternate Names for Livin Antibody

  • baculoviral IAP repeat containing 7
  • baculoviral IAP repeat-containing 7
  • BIRC7
  • KIAP
  • KIAPRING finger protein 50
  • Kidney inhibitor of apoptosis protein
  • livin inhibitor-of-apoptosis
  • Livin
  • Melanoma inhibitor of apoptosis protein
  • ML-IAP
  • MLIAPlivin inhibitor of apoptosis
  • ML-IAPmliap
  • RNF50LIVINbaculoviral IAP repeat-containing protein 7

Background

Livin (BIRC7/KIAP) is a member of the family of inhibitor of apoptosis proteins (IAP) and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain (reviewed in Ma et al 2006 and Crnkovic-Mertens et al 2006). Resistance towards apoptosis is a hallmark of cancer cells, and overexpression of IAPs can contribute to the development of cancer though inhibiting apoptosis (reviewed in Wright et al. 2005). IAP proteins inhibit apoptosis by binding to and inhibiting caspases through their BIR domain(s). Some IAP members, like Liven, also have a RING-type finger motif at their carboxyl-terminal which may enhance anti-apoptotic activity. Many RING finger-containing IAPs possess E3 ubiquitin ligase activity, and it has been shown that Liven acts an E3 ubiquitin ligase for targeting the degradation of Smac/DIABLO. Smac/DIABLO functions by inhibiting IAP-caspase interactions, thereby promoting apoptosis. Thus degradation of Smac/DIABLO is thought to be a mechanism to enhance Livin anti-apoptotic activity, thereby promoting cell survival. Two splicing variants of Livin, alpha and beta, have been identified. The two isoforms are thought to have different anti-apoptotic properties; however, there are conflicting reports are to whether they actually differ in their biological activities. There is accumulating evidence that Liven plays a significant role in a spectrum of tumor types and it is thought that Liven may have potential as a diagnostic and prognostic tumor marker. Liven is expressed at low levels in adult tissues, and relatively higher levels in developmental tissues and in many cancer cells. Certain cancer patients develop autoantibodies against liven and in a number of cancers, Liven is detected only in the tumors but not, or to substantially lower levels, in the corresponding normal adjacent tissue. This antibody recognizes Livin, both isoform alpha and isoform beta. Human Livin isoform alpha is a 298 amino acid (aa) protein, GenBank no. NP_647478. Human Livin isoform beta is a 280 aa protien, GenBank no. NP_071444.1. The antibody will also recognize other isoforms of Livin that contain the peptide immunogen sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IHC, IHC-P, IP
Supplier Logo

Publications for Livin Antibody (NB100-56145)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC-P, WB.


Filter By Application
IHC-P
(3)
WB
(3)
All Applications
Filter By Species
Human
(4)
All Species
Showing Publications 1 - 5 of 5.
Publications using NB100-56145 Applications Species
Altieri B, Sbiera S, Della Casa S et al. Livin/BIRC7 expression as malignancy marker in adrenocortical tumors Oncotarget Dec 21 2016 [PMID: 28030838] (WB, IHC-P, Human) WB, IHC-P Human
Wang J, Zhang X, Wei P et al. Livin, Survivin and Caspase 3 as early recurrence markers in non-muscle-invasive bladder cancer. World J Urol 2014 Mar 05 [PMID: 24595485] (IHC-P, Human)

Details:
Normal bladder and non-muscle-invasive bladder cancer, Figs 1a-b, 2, Tables 1-3
IHC-P Human
Dai CH, Li J, Shi SB et al. Survivin and Smac gene expressions but not livin are predictors of prognosis in non-small cell lung cancer patients treated with adjuvant chemotherapy following surgery. Jpn J Clin Oncol. 2010 Apr [PMID: 20056675] (IHC-P, Human)

Details:
IHC (P): Human non-small lung cancer, Figs 1 and 5; Table 1.
IHC-P Human
Xie J, Xiong L, Tao X et al. Antitumor effects of murine bone marrow-derived dendritic cells infected with xenogeneic livin alpha recombinant adenoviral vectors against Lewis lung carcinoma. Lung Cancer. 2010 Jun [PMID: 19671483] (WB)

Details:
WB (Fig 4): Lane 1, mouse dendritic cells infected with human livin alpha adenovirus (Ad) Lane 2, mock-infected mouse dendritic cells (negative control for livin expression) Lane 3, Lewis lung carcinoma mouse (LLC) cell line Note: the specificity of the a
WB
Liu HB, Kong CZ, Zeng Y et al. Livin may serve as a marker for prognosis of bladder cancer relapse and a target of bladder cancer treatment. Urol Oncol. 2009 May-Jun [PMID: 18555709] (WB, Human)

Details:
WB: Fig 1 (human bladder cancer tissues) and Fig 2 (human livin alpha transfected human bladder cell lines, untransfected cell lines were used as a negative control). Note: the specificity of the antibody was validated by WB in human livin alpha transfect
WB Human

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Video Protocols

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Blogs on Livin.

Survivin - an inhibitor of apoptosis protein
Survivin is an anti-apoptotic protein which is the smallest protein within a large family of proteins including X-linked IAP, c-IAP1 and 2, IAP-like protein-2, melanoma IAP, NAIP, and Livin. Survivin is responsible for a wide range of basic cellula...  Read full blog post.

Survivin is thrivin'
The survivin anti-apoptotic protein is the smallest member of a large family of proteins such as X-linked IAP, c-IAP1 and 2, IAP-like protein-2, melanoma IAP, Livin, and NAIP. Survivin regulates basic physiological events such as the cell cycle, tumor...  Read full blog post.

Livin: On a Prayer
Livin is a member of the inhibitor of apoptosis proteins (IAP) family that regulates programmed cell death. The Livin protein contains a single baculovirus IAP repeat (BIR) essential for function, along with a COOH-terminal RING-type zinc finger domai...  Read full blog post.

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Bioinformatics

Gene Symbol BIRC7
Entrez
Uniprot