LIS1 Antibody - BSA Free

Western Blot: LIS1 Antibody [NB200-326] - Detection of Human, Mouse and Rat LIS-1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, K562, mouse TCMK-1, and rat C6 cells. Antibodies: Affinity purified rabbit anti-LIS-1 antibody NB200-326 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Western Blot: LIS1 Antibody [NB200-326] - Whole cell lysate (50 ug) from TF-1 (erythroleukemia) cells. Antibody used at the indicated concentrations.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

LIS1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit LIS1 Antibody - BSA Free (NB200-326) is a polyclonal antibody validated for use in WB. Anti-LIS1 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Rabbits were immunized with a synthetic peptide, which represented a portion of human Lissencephaly-1 protein encoded in part by exons 3 and 4 (LocusLink ID 5048). Antibody was affinity purified using the peptide immobilized on solid support.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PAFAH1B1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:300-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LIS1 Antibody - BSA Free

• LIS-1
• LIS1PAF-AH alpha
• LIS2
• lissencephaly 1 protein
• Lissencephaly-1 protein
• MDCR
• MDS
• Miller-Dieker syndrome chromosome region
• PAF acetylhydrolase 45 kDa subunit
• PAF-AH 45 kDa subunit
• PAFAH alpha
• PAFAH
• PAFAHA
• platelet-activating factor acetylhydrolase 1b, regulatory subunit 1 (45kDa)
• platelet-activating factor acetylhydrolase IB subunit alpha
• platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit (45kD)
• platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45kDa
• platelet-activating factor acetylhydrolase, isoform Ib, subunit 1 (45kDa)

Background

Lissencephaly (LIS), literally meaning smooth brain, has multiple causes. Agyria, i.e., brain without convolutions or gyri, was considered a rare malformation until recent progress in neuroradiology. With this technical advantage, a number of lissencephaly syndromes have been distinguished. Classic lissencephaly (type I) is a brain malformation caused by abnormal neuronal migration at 9 to 13 weeks' gestation, resulting in a spectrum of agyria, mixed agyria / pachygyria, and pachygyria. It is characterized by an abnormally thick and poorly organized cortex with 4 primitive layers, diffuse neuronal heterotopia, enlarged and dysmorphic ventricles, and often hypoplasia of the corpus callosum. Lissencephaly is found in association with facial abnormalities in Miller-Dieker syndrome and without other major anomalies in X-linked lissencephaly and isolated lissencephaly sequence (ILS). Subcortical band heterotopia (SBH) are bilateral and symmetric ribbons of gray matter found in the central white matter between the cortex and the ventricular surface and comprise the less severe end of the lissencephaly spectrum of malformations.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LIS1 Antibody (NB200-326)(3)

Publications using NB200-326

• Alkuraya FS, Cai X, Emery C et al. Human mutations in NDE1 cause extreme microcephaly with lissencephaly [corrected]. Am J Hum Genet 2011-05-01 [PMID: 21529751]
• Lam C, Vergnolle MA, Thorpe L et al. Functional interplay between LIS1, NDE1 and NDEL1 in dynein-dependent organelle positioning. J Cell Sci 2010-01-01 [PMID: 20048338]
• Green RA, Wollman R, Kaplan KB et al. APC and EB1 function together in mitosis to regulate spindle dynamics and chromosome alignment. Mol Biol Cell 2005-10-01 [PMID: 16030254]

Bioinformatics

• Gene Symbol: PAFAH1B1
• Entrez:
  • 5048(Human)