Lipocalin-2/NGAL Antibody

Western blot shows lysates of rat ovary tissue and mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Mouse/Rat Lipocalin‑2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3508) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Lipocalin‑2/NGAL at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Rt
• Applications: WB, IP, ELISA(Cap)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Lipocalin-2/NGAL Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat Lipocalin-2/NGAL
  Gln21-Asn198
  Accession # P30152
• Specificity: Detects rat Lipocalin-2/NGAL in ELISAs and detects rat and mouse Lipocalin-2/NGAL in Western blots. In sandwich ELISAs, less than 0.4% cross-reactivity with recombinant mouse (rm) Lipocalin-2/NGAL-2 is observed and less than 0.2% cross-reactivity with recombinant human (rh) Lipocalin-1/NGAL-1, rhLipocalin-2/NGAL-2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LCN2
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: ELISA Capture: Rat Lipocalin-2/NGAL Antibody (Catalog # AF3508)
  ELISA Detection: Rat Lipocalin-2/NGAL Biotinylated Antibody (Catalog # BAF3508)
  Standard: Recombinant Rat Lipocalin-2/NGAL (Catalog # 3508-LC)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lipocalin-2/NGAL Antibody

• 24p3
• 25 kDa alpha-2-microglobulin-related subunit of MMP-9
• HNL
• LCN2
• lipocalin 2 (oncogene 24p3)
• lipocalin 2
• Lipocalin2
• Lipocalin-2
• migration-stimulating factor inhibitor
• MSFI
• neutrophil gelatinase-associated lipocalin
• NGAL
• NGALlipocalin-2
• Oncogene 24p3
• p25
• Siderocalin
• Uterocalin

Background

Lipocalin-2, also known as neutrophil gelatinase-associated Lipocalin and uterocalin (NGAL), has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (1‑3). It binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (K_D = 0.41 nM) (4). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less K_D values (7.9 nM) (4). Secretion of Lipocalin-2 in immune cells increases by stimulation of Toll-like receptor as a acute phase response to infection. As a result, it acts as a potent bacteriostatic reagents by sequestering iron (5). Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (6). In the kidney, Lipocalin-2-mediated iron trafficking may be involved in protection from renal injury, and it has been implicated as a marker for early kidney failure (7, 8).

1. Kjeldsen L, et al. (2000) Biochim Biophys Acta. 1482:272.
2. Devireddy, L.R. et al. (2001) Science 293:829.
3. Yang, M.B. et al. (2002) Mol. Cell. 10:1045.
4. Goetz, D.H. et al. (2002) Mol. Cell 10:1033.
5. Flo, T.H. et al. (2004) Nature 432:917.
6. Hanai, J. et al. (2005) J. Biol. Chem. 280:13641.
7. Mori, K. et al. (2005) J. Clin. Invest. 115:610.
8. Mishra, J. et al. (2005) Lancet 365:1231.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lipocalin-2/NGAL Antibody (AF3508)(6)

Publications using AF3508

• C Poggel, T Adams, R Janzen, A Hofmann, O Hardt, E Roeb, SK Schröder, CG Tag, M Roderfeld, R Weiskirche Isolation of Hepatocytes from Liver Tissue by a Novel, Semi-Automated Perfusion Technology Biomedicines, 2022;10(9):. 2022 [PMID: 36140299] (Western Blot, Mouse)
• JY Lim, WW Jung, W Kim, KS Moon, D Sul Nephrotoxicity evaluation and proteomic analysis in kidneys of rats exposed to thioacetamide Scientific Reports, 2022;12(1):6837. 2022 [PMID: 35477741] (Western Blot, Rat)
• P Klüber, SK Meurer, J Lambertz, R Schwarz, S Zechel-Gra, T Braunschwe, S Hurka, E Domann, R Weiskirche Depletion of Lipocalin 2 (LCN2) in Mice Leads to Dysbiosis and Persistent Colonization with Segmented Filamentous Bacteria International Journal of Molecular Sciences, 2021;22(23):. 2021 [PMID: 34884961] (Western Blot, Mouse)
• A Asimakopou, E Borkham-Ka, EV de Leur, R Weiskirche Data on Lipocalin 2 and phosphatidylinositol 3-kinase signaling in a methionine- and choline-deficient model of non-alcoholic steatohepatitis Data Brief, 2017;13(0):644-649. 2017 [PMID: 28725667] (Western Blot, Mouse)
• Chia W, Tan F, Ong W, Dawe G Expression and localisation of brain-type organic cation transporter (BOCT/24p3R/LCN2R) in the normal rat hippocampus and after kainate-induced excitotoxicity. Neurochem Int, 0;87(0):43-59. 0 [PMID: 26004810] (Western Blot, Rat)
• Soler-Garcia AA, Johnson D, Hathout Y, Ray PE Iron-related proteins: candidate urine biomarkers in childhood HIV-associated renal diseases. Clin J Am Soc Nephrol, 2009;4(4):763-71. 2009 [PMID: 19279121] (ELISA Development, Rat)

Review for Lipocalin-2/NGAL Antibody (AF3508) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Anonymous - WB Rat 01/07/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 21457438
• Species: Rat

Bioinformatics

• Gene Symbol: LCN2
• Uniprot:
  • P30152()