Lipocalin-2/NGAL Antibody

Images

 
Western blot shows lysates of rat ovary tissue and mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Mouse/Rat Lipocalin‑2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3508) followed by ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, IP, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Lipocalin-2/NGAL Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant rat Lipocalin-2/NGAL
Gln21-Asn198
Accession # P30152
Specificity
Detects rat Lipocalin-2/NGAL in ELISAs and detects rat and mouse Lipocalin-2/NGAL in Western blots. In sandwich ELISAs, less than 0.4% cross-reactivity with recombinant mouse (rm) Lipocalin-2/NGAL-2 is observed and less than 0.2% cross-reactivity with recombinant human (rh) Lipocalin-1/NGAL-1, rhLipocalin-2/NGAL-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LCN2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunoprecipitation 25 ug/mL
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture: Rat Lipocalin-2/NGAL Antibody (Catalog # AF3508)
ELISA Detection: Rat Lipocalin-2/NGAL Biotinylated Antibody (Catalog # BAF3508)
Standard: Recombinant Rat Lipocalin-2/NGAL (Catalog # 3508-LC)
Reviewed Applications
Read 1 Review rated 5
using
AF3508 in the following applications:

Publications
Read Publications using
AF3508 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lipocalin-2/NGAL Antibody

  • 24p3
  • 25 kDa alpha-2-microglobulin-related subunit of MMP-9
  • HNL
  • LCN2
  • lipocalin 2 (oncogene 24p3)
  • lipocalin 2
  • Lipocalin2
  • Lipocalin-2
  • migration-stimulating factor inhibitor
  • MSFI
  • neutrophil gelatinase-associated lipocalin
  • NGAL
  • NGALlipocalin-2
  • Oncogene 24p3
  • p25
  • Siderocalin
  • Uterocalin

Background

Lipocalin-2, also known as neutrophil gelatinase-associated Lipocalin and uterocalin (NGAL), has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (1‑3). It binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (K= 0.41 nM) (4). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less KD values (7.9 nM) (4). Secretion of Lipocalin-2 in immune cells increases by stimulation of Toll-like receptor as a acute phase response to infection. As a result, it acts as a potent bacteriostatic reagents by sequestering iron (5). Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (6). In the kidney, Lipocalin-2-mediated iron trafficking may be involved in protection from renal injury, and it has been implicated as a marker for early kidney failure (7, 8).

  1. Kjeldsen L, et al. (2000) Biochim Biophys Acta. 1482:272.
  2. Devireddy, L.R. et al. (2001) Science 293:829.
  3. Yang, M.B. et al. (2002) Mol. Cell. 10:1045.
  4. Goetz, D.H. et al. (2002) Mol. Cell 10:1033.
  5. Flo, T.H. et al. (2004) Nature 432:917.
  6. Hanai, J. et al. (2005) J. Biol. Chem. 280:13641.
  7. Mori, K. et al. (2005) J. Clin. Invest. 115:610.
  8. Mishra, J. et al. (2005) Lancet 365:1231.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lipocalin-2/NGAL Antibody (AF3508)(3)

Review for Lipocalin-2/NGAL Antibody (AF3508) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using AF3508:
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WB
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Rat 01/07/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 21457438
SpeciesRat

Blocking

Blocking DetailsSee PMID 21457438

Details

Detection NotesSee PMID 21457438

Comments

CommentsPublished: See PMID 21457438Independent review from antibodyresource.comhigh quality antibody

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Biotin BAF3508

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Anonymous
01/07/2015
Application: WB
Species: Rat

Bioinformatics

Gene Symbol LCN2
Uniprot