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Lipocalin-2/NGAL Antibody

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Western blot shows lysates of rat ovary tissue and mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Mouse/Rat Lipocalin-2/NGAL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3508) followed by ...read more
Visualization of expression of selected proteins by Western blot analysis. Protein extracts were prepared from livers of WT and Lcn2-/- mice that were subjected to indicated diets for 4 or 6 weeks (n=3 animals/group). ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, IP, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Lipocalin-2/NGAL Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant rat Lipocalin-2/NGAL
Gln21-Asn198
Accession # P30152
Specificity
Detects rat Lipocalin-2/NGAL in ELISAs and detects rat and mouse Lipocalin-2/NGAL in Western blots. In sandwich ELISAs, less than 0.4% cross-reactivity with recombinant mouse (rm) Lipocalin-2/NGAL-2 is observed and less than 0.2% cross-reactivity with recombinant human (rh) Lipocalin-1/NGAL-1, rhLipocalin-2/NGAL-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LCN2
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL
Application Notes
ELISA Capture: Rat Lipocalin-2/NGAL Antibody (Catalog # AF3508)
ELISA Detection: Rat Lipocalin-2/NGAL Biotinylated Antibody (Catalog # BAF3508)
Standard: Recombinant Rat Lipocalin-2/NGAL (Catalog # 3508-LC)
Reviewed Applications
Read 1 Review rated 5
using
AF3508 in the following applications:

Publications
Read Publications using
AF3508 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lipocalin-2/NGAL Antibody

  • 24p3
  • 25 kDa alpha-2-microglobulin-related subunit of MMP-9
  • HNL
  • LCN2
  • lipocalin 2 (oncogene 24p3)
  • lipocalin 2
  • Lipocalin2
  • Lipocalin-2
  • migration-stimulating factor inhibitor
  • MSFI
  • neutrophil gelatinase-associated lipocalin
  • NGAL
  • NGALlipocalin-2
  • Oncogene 24p3
  • p25
  • Siderocalin
  • Uterocalin

Background

Lipocalin-2, also known as neutrophil gelatinase-associated Lipocalin and uterocalin (NGAL), has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (1‑3). It binds a bacterial catecholate sidropore bound to ferric ion such as enterobactin with a subnanomolar dissociation constant (K= 0.41 nM) (4). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less KD values (7.9 nM) (4). Secretion of Lipocalin-2 in immune cells increases by stimulation of Toll-like receptor as a acute phase response to infection. As a result, it acts as a potent bacteriostatic reagents by sequestering iron (5). Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (6). In the kidney, Lipocalin-2-mediated iron trafficking may be involved in protection from renal injury, and it has been implicated as a marker for early kidney failure (7, 8).

  1. Kjeldsen L, et al. (2000) Biochim Biophys Acta. 1482:272.
  2. Devireddy, L.R. et al. (2001) Science 293:829.
  3. Yang, M.B. et al. (2002) Mol. Cell. 10:1045.
  4. Goetz, D.H. et al. (2002) Mol. Cell 10:1033.
  5. Flo, T.H. et al. (2004) Nature 432:917.
  6. Hanai, J. et al. (2005) J. Biol. Chem. 280:13641.
  7. Mori, K. et al. (2005) J. Clin. Invest. 115:610.
  8. Mishra, J. et al. (2005) Lancet 365:1231.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Lipocalin-2/NGAL Antibody (AF3508)(14)

We have publications tested in 3 confirmed species: Mouse, Rat, Transgenic Mouse.

We have publications tested in 4 applications: ELISA Development, IHC, IHC-Fr, Western Blot.


Filter By Application
ELISA Development
(1)
IHC
(2)
IHC-Fr
(1)
Western Blot
(7)
All Applications
Filter By Species
Mouse
(4)
Rat
(3)
Transgenic Mouse
(3)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using AF3508 Applications Species
Kessel, JC;Weiskirchen, R;Schröder, SK; Expression Analysis of Lipocalin 2 (LCN2) in Reproductive and Non-Reproductive Tissues of Esr1-Deficient Mice International journal of molecular sciences 2023-05-25 [PMID: 37298232] (Western Blot, IHC, Transgenic Mouse) Western Blot, IHC Transgenic Mouse
J Wang, Z Zhang, J Guan, HC Tung, J Xie, H Huang, Y Chen, M Xu, S Ren, S Li, M Zhang, D Yang, W Xie Hepatocyte estrogen sulfotransferase inhibition protects female mice from Concanavalin A-induced T cell-mediated hepatitis independent of estrogens The Journal of Biological Chemistry, 2023-02-15;0(0):103026. 2023-02-15 [PMID: 36796516] (IHC, Western Blot, Mouse, Transgenic Mouse) IHC, Western Blot Mouse, Transgenic Mouse
C Poggel, T Adams, R Janzen, A Hofmann, O Hardt, E Roeb, SK Schröder, CG Tag, M Roderfeld, R Weiskirche Isolation of Hepatocytes from Liver Tissue by a Novel, Semi-Automated Perfusion Technology Biomedicines, 2022-09-06;10(9):. 2022-09-06 [PMID: 36140299] (Western Blot, Mouse) Western Blot Mouse
JY Lim, WW Jung, W Kim, KS Moon, D Sul Nephrotoxicity evaluation and proteomic analysis in kidneys of rats exposed to thioacetamide Scientific Reports, 2022-04-27;12(1):6837. 2022-04-27 [PMID: 35477741] (Western Blot, Rat) Western Blot Rat
P Klüber, SK Meurer, J Lambertz, R Schwarz, S Zechel-Gra, T Braunschwe, S Hurka, E Domann, R Weiskirche Depletion of Lipocalin 2 (LCN2) in Mice Leads to Dysbiosis and Persistent Colonization with Segmented Filamentous Bacteria International Journal of Molecular Sciences, 2021-12-05;22(23):. 2021-12-05 [PMID: 34884961] (Western Blot, Mouse, Transgenic Mouse) Western Blot Mouse, Transgenic Mouse
Asimakopoulou A, Vucur M, Luedde T et al. Perilipin 5 and Lipocalin 2 Expression in Hepatocellular Carcinoma Cancers (Basel) 3/19/2019 [PMID: 30893876]
Erawan Borkham-Kamphorst, Eddy Van de Leur, Steffen K. Meurer, Eva M. Buhl, Ralf Weiskirchen N-Glycosylation of Lipocalin 2 Is Not Required for Secretion or Exosome Targeting Frontiers in Pharmacology 2018-04-25 [PMID: 29755357]
J Lambertz, T Berger, TW Mak, J van Helden, R Weiskirche Lipocalin-2 in Fructose-Induced Fatty Liver Disease Front Physiol, 2017-11-28;8(0):964. 11/28/2017 [PMID: 29234288]
A Asimakopou, E Borkham-Ka, EV de Leur, R Weiskirche Data on Lipocalin 2 and phosphatidylinositol 3-kinase signaling in a methionine- and choline-deficient model of non-alcoholic steatohepatitis Data Brief, 2017-07-01;13(0):644-649. 2017-07-01 [PMID: 28725667] (Western Blot, Mouse) Western Blot Mouse
Josef G. Heuer, Shannon M. Harlan, Derek D. Yang, Dianna L. Jaqua, Jeffrey S. Boyles, Jonathan M. Wilson et al. Role of TGF-alpha in the progression of diabetic kidney disease American Journal of Physiology-Renal Physiology 2017-06-01 [PMID: 28249836]
Show All 14 Publications.

Review for Lipocalin-2/NGAL Antibody (AF3508) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using AF3508:
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WB
(1)
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Rat
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
WB Rat 01/07/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 21457438
SpeciesRat

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Verified Customer
01/07/2015
Application: WB
Species: Rat

Bioinformatics

Gene Symbol LCN2
Uniprot