Lipocalin-2/NGAL Antibody (32) - BSA Free

ELISA: Lipocalin-2/NGAL Antibody (32) - BSA Free [NBP1-05182] - The figure illustrates the calibration curve of a sandwich assay with NBP1-05182 as the capture antibody and NBP1-05182B as the biotinylated detection antibody.

• Reactivity: Rt
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P
• Clone: 32
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Lipocalin-2/NGAL Antibody (32) - BSA Free Summary

• Immunogen: Recombinant rat NGAL precursor
• Specificity: Binds rat NGAL (neutrophil gelatinase-associated lipocalin).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: LCN2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:4000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Western Blot 1:100-1:2000
• Application Notes: ELISA: NBP1-05182 binds free rat NGAL in solution. WB: NBP1-05182 can be used in Western blotting. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 27136530). ICC/IF reactivity reported in scientific literature (PMID: 27136530).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for Lipocalin-2/NGAL Antibody (32) - BSA Free

• 24p3
• 25 kDa alpha-2-microglobulin-related subunit of MMP-9
• HNL
• LCN2
• lipocalin 2 (oncogene 24p3)
• lipocalin 2
• Lipocalin2
• Lipocalin-2
• migration-stimulating factor inhibitor
• MSFI
• neutrophil gelatinase-associated lipocalin
• NGAL
• NGALlipocalin-2
• Oncogene 24p3
• p25
• Siderocalin
• Uterocalin

Background

Rat NGAL, so called because it is the ortholog of human neutrophil gelatinase-associated lipocalin (NGAL), is also called lipocalin 2, 25-kDa alpha 2-microglobulin-related protein or neu-related lipocalin. It is expressed by neutrophils and various epithelial cells, including cells of the renal proximal tubules. It is involved in physiological processes such as the binding of siderophore iron and tissue growth and regeneration. It is upregulated in a variety of pathological situations including infection, inflammation, ischemia-reperfusion and in certain adenocarcinomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lipocalin-2/NGAL Antibody (NBP1-05182)(2)

Publications using NBP1-05182

• Sultan S, Ahmad S, Rave-Frank M et al. Induction of Lipocalin2 in a Rat Model of Lung Irradiation. Int J Mol Sci. 2016-05-04 [PMID: 27136530] (WB, ICC/IF, IHC-P, Rat)
• Ahmad S. Effect of acute phase cytokines on iron uptake in hepatocytes and differential localization of Lipocalin-2 and Transferrin receptors in rat hepatic and extra hepatic organs. Thesis. 2014-03-24

Review for Lipocalin-2/NGAL Antibody (NBP1-05182) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Hyun Yi WB Rat 04/20/2017

Summary

• Application: Western Blot
• Sample Tested: HAPI
• Species: Rat
• Lot: 10613

Bioinformatics

• Gene Symbol: LCN2