Lipocalin-2/NGAL Antibody (228421)

Epitope mapping of LCN2 mAb. (A) ELISA quantification of relative binding of LCN2 mAb to 56 peptides covering the mouse LCN2 protein (n = 3); (B) The combined sequences of peptides 22–24 are shown in red at amino acids 64–84 of the mouse LCN2 protein. The N-terminal signal peptide that was cleaved prior to the secretion of the protein is underlined and not included in the epitope mapping. Cys-78 and Cys-177 forming an intramolecular disulfide bond (blue); (C) Alignment of the LCN2 mAb epitope sequences from mouse, rat, and human LCN2 homologs. Conserved amino acids are underlined. Amino acids interacting with siderophore are highlighted in pink. Conserved cysteines are highlighted in blue; (D) The crystal structure of human LCN2 protein containing a siderophore (black) and an iron (orange). The epitope of LCN2 mAb, highlighted in red, is located in the beta 3 and beta 4 strands. The side chains of amino acids bound with siderophore are colored in pink. An intramolecular disulfide bond formed between Cys-78 in the epitope and Cys-177 near the C-terminal (blue); (E) Representative Western blot showing that increased concentrations of epitope peptides from mouse (YNVTSILVRDQDQGCRYWIRT) and human (YNVTSVLFRKKKCDYWIRT) tissue reduced the level of immunoprecipitated mouse (mLCN2) or human LCN2 protein (hLCN2). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32872405), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: ELISA(Cap)
• Clone: 228421
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Lipocalin-2/NGAL Antibody (228421) Summary

• Immunogen: NS0-derived recombinant mouse Lipocalin-2/NGAL
  Gln21-Asn200
  Accession # P11672
• Specificity: Detects mouse Lipocalin-2/NGAL in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human (rh) Lipocalin-1, rhLipocalin-2 or recombinant rat Lipocalin-2 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: LCN2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Capture: Mouse Lipocalin-2/NGAL Antibody (Catalog # MAB18571)
  ELISA Detection: Mouse Lipocalin-2/NGAL Biotinylated Antibody (Catalog # BAM1857)
  Standard: Recombinant Mouse Lipocalin-2/NGAL (Catalog # 1857-LC)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lipocalin-2/NGAL Antibody (228421)

• 24p3
• 25 kDa alpha-2-microglobulin-related subunit of MMP-9
• HNL
• LCN2
• lipocalin 2 (oncogene 24p3)
• lipocalin 2
• Lipocalin2
• Lipocalin-2
• migration-stimulating factor inhibitor
• MSFI
• neutrophil gelatinase-associated lipocalin
• NGAL
• NGALlipocalin-2
• Oncogene 24p3
• p25
• Siderocalin
• Uterocalin

Background

Mouse Lipocalin-2 was cloned from mouse kidney cells (1). Its very high level of expression at the post-stratum uterus gave it the name uterocalin (2). Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (3‑5). Studies indicate that Lipocalin-2 binds a bacterial catecholate siderophore that is bound to a ferric ion, such as enterobactin, with a subnanomolar dissociation constant (K_D = 0.41 nM) (6). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less K_D values (7.9 nM) (6). Secretion of Lipocalin-2 in immune cells increases in response to stimulation of Toll-like receptor as an acute phase response to infection. As a result, it acts as a potent bacteriostatic reagent by sequestering iron (7). Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing the epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (8).

1. Hraba-Renevey, s. et al. (1989) Oncogene. 4:601. 
2. Liu, Q. et al. (1993) Mol Reprod Dev. 46:507. 
3. Kjeldsen L, et al. (2000) Biochim Biophys Acta. 1482:272.
4. Devireddy, L.R. et al. (2001) Science 293:829.
5. Yang, M.B. et al. (2002) Mol. Cell. 10:1045.
6. Goetz, D.H. et al. (2002) Mol. Cell 10:1033.
7. Flo, T.H. et al. (2004) Nature 432:917.
8. Hanai, J. et al. (2005) J. Biol. Chem. 280:13641.

 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lipocalin-2/NGAL Antibody (MAB18571)(4)

Publications using MAB18571

• Guona Wang, Yi-Chinn Weng, I-Chen Chiang, Yu-Ting Huang, Yi-Chu Liao, Yi-Chun Chen et al. Neutralization of Lipocalin-2 Diminishes Stroke-Reperfusion Injury International Journal of Molecular Sciences 8/29/2020 [PMID: 32872405]
• Guona Wang, Yi-Chinn Weng, I-Chen Chiang, Yu-Ting Huang, Yi-Chu Liao, Yi-Chun Chen et al. Neutralization of Lipocalin-2 Diminishes Stroke-Reperfusion Injury International Journal of Molecular Sciences 2020-08-29 [PMID: 32872405] (Immunoprecipitation, Neutralization, ELISA Capture, Transgenic Mouse)
• J Yan, SP Pandey, BJ Barnes, JR Turner, C Abraham T Cell-Intrinsic IRF5 Regulates T Cell Signaling, Migration, and Differentiation and Promotes Intestinal Inflammation Cell Rep, 2020-06-30;31(13):107820. 2020-06-30 [PMID: 32610123] (Western Blot, Mouse)
• Cheng L, Xing H, Mao X, Li L, Li X, Li Q Lipocalin-2 promotes m1 macrophages polarization in a mouse cardiac ischaemia-reperfusion injury model. Scand J Immunol, 2015-01-01;81(1):31-8. 2015-01-01 [PMID: 25359467] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: LCN2
• Uniprot:
  • P11672()