Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Protein A or G purified from hybridoma culture supernatant
Reconstitute at 0.5 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Lipocalin-2/NGAL Antibody (228421)
25 kDa alpha-2-microglobulin-related subunit of MMP-9
lipocalin 2 (oncogene 24p3)
migration-stimulating factor inhibitor
neutrophil gelatinase-associated lipocalin
Mouse Lipocalin-2 was cloned from mouse kidney cells (1). Its very high level of expression at the post-stratum uterus gave it the name uterocalin (2). Lipocalin-2 has been implicated in a variety of processes including cell differentiation, tumorigenesis, and apoptosis (3‑5). Studies indicate that Lipocalin-2 binds a bacterial catecholate siderophore that is bound to a ferric ion, such as enterobactin, with a subnanomolar dissociation constant (KD = 0.41 nM) (6). The bound ferric enterobactin complex breaks down slowly in a month into dihydroxybenzoyl serine and dihydroxybenzoic acid (DHBA). It also binds to a ferric DHBA complex with much less KD values (7.9 nM) (6). Secretion of Lipocalin-2 in immune cells increases in response to stimulation of Toll-like receptor as an acute phase response to infection. As a result, it acts as a potent bacteriostatic reagent by sequestering iron (7). Moreover, Lipocalin-2 can alter the invasive and metastatic behavior of Ras-transformed breast cancer cells in vitro and in vivo by reversing the epithelial to mesenchymal transition inducing activity of Ras, through restoration of E-cadherin expression, via effects on the Ras-MAPK signaling pathway (8).
Hraba-Renevey, s. et al. (1989) Oncogene. 4:601.
Liu, Q. et al. (1993) Mol Reprod Dev. 46:507.
Kjeldsen L, et al. (2000) Biochim Biophys Acta. 1482:272.
Devireddy, L.R. et al. (2001) Science 293:829.
Yang, M.B. et al. (2002) Mol. Cell. 10:1045.
Goetz, D.H. et al. (2002) Mol. Cell 10:1033.
Flo, T.H. et al. (2004) Nature 432:917.
Hanai, J. et al. (2005) J. Biol. Chem. 280:13641.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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