Lipocalin-2/NGAL Antibody (228418) [Unconjugated]

Western blot shows lysates of mouse spleen tissue, mouse uterus tissue, and mouse epididymis tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Lipocalin‑2/NGAL Monoclonal Antibody (Catalog # MAB1857) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Lipocalin‑2/NGAL at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu
• Applications: WB
• Clone: 228418
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

Lipocalin-2/NGAL Antibody (228418) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Lipocalin-2
  aa 21-200
  Accession # P11672
• Specificity: Detects recombinant mouse (rm)Lipocalin-2 in direct ELISAs and Western blots. In these formats, does not cross-react with rhLipocalin-1 or rhLipocalin‑2.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: LCN2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lipocalin-2/NGAL Antibody (228418) [Unconjugated]

• 24p3
• 25 kDa alpha-2-microglobulin-related subunit of MMP-9
• HNL
• LCN2
• lipocalin 2 (oncogene 24p3)
• lipocalin 2
• Lipocalin2
• Lipocalin-2
• migration-stimulating factor inhibitor
• MSFI
• neutrophil gelatinase-associated lipocalin
• NGAL
• NGALlipocalin-2
• Oncogene 24p3
• p25
• Siderocalin
• Uterocalin

Background

Lipocalin-2, also known as Neutrophil Gelatin-Associated Lipocalin (NGAL) and Siderocalin, mediates the innate immune response to bacterial infection by sequestering iron-laden siderophores.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lipocalin-2/NGAL Antibody (MAB1857)(11)

Publications using MAB1857

• C Huisman, MA Norgard, PR Levasseur, SM Krasnow, MGP van der Wi, B Olson, DL Marks Critical changes in hypothalamic gene networks in response to pancreatic cancer as found by single-cell RNA sequencing Molecular Metabolism, 2022;0(0):101441. 2022-01-01 [PMID: 35031523] (IHC, Mouse)
• C Mertens, L Kuchler, A Sola, R Guiteras, S Grein, B Brüne, A von Knethe, M Jung Macrophage-Derived Iron-Bound Lipocalin-2 Correlates with Renal Recovery Markers Following Sepsis-Induced Kidney Damage Int J Mol Sci, 2020;21(20):. 2020-01-01 [PMID: 33065981] (Immunoprecipitation, Mouse)
• A Braga, S Bandiera, J Verheyen, R Hamel, C Rutigliani, F Edenhofer, JA Smith, S Pluchino Combination of In Situ Lcn2 pRNA-RNAi Nanotherapeutics and iNSC Transplantation Ameliorates Experimental SCI in Mice Mol. Ther., 2020;0(0):. 2020-01-01 [PMID: 32877696] (Western Blot, Mouse)
• D Fischer, C Seifen, P Baer, M Jung, C Mertens, B Scheller, K Zacharowsk, R Hofmann, TJ Maier, A Urbschat The Fibrin Cleavage Product B?15-42 Channels Endothelial and Tubular Regeneration in the Post-acute Course During Murine Renal Ischemia Reperfusion Injury Front Pharmacol, 2018;9(0):369. 2018-01-01 [PMID: 29755348] (IHC, Mouse)
• JA Smith, A Braga, J Verheyen, S Basilico, S Bandiera, C Alfaro-Cer, L Peruzzotti, D Shu, F Haque, P Guo, S Pluchino RNA Nanotherapeutics for the Amelioration of Astroglial Reactivity Mol Ther Nucleic Acids, 2018;10(0):103-121. 2018-01-01 [PMID: 29499926] (ICC, Mouse)
• R Xiao, R Chen Neutrophil gelatinase?associated lipocalin as a potential novel biomarker for ventilator?associated lung injury Mol Med Rep, 2017;15(6):3535-3540. 2017-01-01 [PMID: 28393227] (Western Blot, Mouse)
• S Gomez-Chou, A Swidnicka-, N Badi, M Chavez-Tom, GB Lesinski, T Bekaii-Saa, MR Farren, TA Mace, C Schmidt, Y Liu, D Deng, RF Hwang, L Zhou, TT Moore, D Chatterjee, H Wang, X Leng, RB Arlinghaus, CD Logsdon, Z Cruz-Monse Lipocalin-2 Promotes Pancreatic Ductal Adenocarcinoma by Regulating Inflammation in the Tumor Microenvironment Cancer Res, 2017;0(0):. 2017-01-01 [PMID: 28249896] (Western Blot, Mouse)
• Shashidharamurthy R, Machiah D, Aitken J, Putty K, Srinivasan G, Chassaing B, Parkos C, Selvaraj P, Vijay-Kumar M Differential role of lipocalin 2 during immune complex-mediated acute and chronic inflammation in mice. Arthritis Rheum, 2013;65(4):1064-73. 2013-01-01 [PMID: 23280250] (Neutralization, Mouse)
• te Boekhorst BC, Bovens SM, Hellings WE, van der Kraak PH, van de Kolk KW, Vink A, Moll FL, van Oosterhout MF, de Vries JP, Doevendans PA, Goumans MJ, de Kleijn DP, van Echteld CJ, Pasterkamp G, Sluijter JP Molecular MRI of murine atherosclerotic plaque targeting NGAL: a protein associated with unstable human plaque characteristics. Cardiovasc. Res., 2011;89(3):680-8. 2011-01-01 [PMID: 21030534] (IHC-P, Mouse)
• Nairz M, Theurl I, Schroll A, Theurl M, Fritsche G, Lindner E, Seifert M, Crouch ML, Hantke K, Akira S, Fang FC, Weiss G Absence of functional Hfe protects mice from invasive Salmonella enterica serovar Typhimurium infection via induction of lipocalin-2. Blood, 2009;114(17):3642-51. 2009-01-01 [PMID: 19700664] (Immunoprecipitation, Mouse)
• Nairz M, Fritsche G, Brunner P, Talasz H, Hantke K, Weiss G Interferon-gamma limits the availability of iron for intramacrophage Salmonella typhimurium. Eur. J. Immunol., 2008;38(7):1923-36. 2008-01-01 [PMID: 18581323] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: LCN2
• Uniprot:
  • P11672()