Lipocalin-2/NGAL Antibody (14) - BSA Free

ELISA: Lipocalin-2/NGAL Antibody (14) - BSA Free [NBP1-30002] - The calibration curve of a sandwich assay for Human Dimer NGAL using NBP1-30002 as the capture antibody and NBP1-30002B as the biotinylated detection antibody.

Product Discontinued

Lipocalin-2/NGAL Antibody (14) - BSA Free Summary

• Immunogen: Recombinant human NGAL
• Epitope: Differs from those of NBP2-41364, NBP2-41365, NBP2-41368 antibodies.
• Specificity: Binds human NGAL (neutrophil gelatinase-associated lipocalin).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: LCN2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:4000
  • Western Blot 1:1000
• Application Notes: ELISA: NBP1-30002 binds human NGAL in solution and coated onto a solid phase. NBP1-30002 (as capture antibody) forms sandwich pairs with NBP2-23529, NBP2-23531, NBP1-30000,NBP1-30003 and NBP1-30004 (as biotinylated detection antibodies) for measuring human NGAL. With NBP1-30003B detection antibody, the assay reacts equally with NGAL monomer and homodimer on a mass basis. With NBP1-30002B detection antibody, the assay is specific for NGAL homodimer and can be used at sample dilutions giving low levels of total NGAL. WB: NBP1-30002 can be used in Western blotting.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 10mM Phosphate (pH 7.4) and 0.5M NaCl
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for Lipocalin-2/NGAL Antibody (14) - BSA Free

• 24p3
• 25 kDa alpha-2-microglobulin-related subunit of MMP-9
• HNL
• LCN2
• lipocalin 2 (oncogene 24p3)
• lipocalin 2
• Lipocalin2
• Lipocalin-2
• migration-stimulating factor inhibitor
• MSFI
• neutrophil gelatinase-associated lipocalin
• NGAL
• NGALlipocalin-2
• Oncogene 24p3
• p25
• Siderocalin
• Uterocalin

Background

Lipocalin 2 is a member of the lipocalin family which encompass more than 25 members (including proteins like retinol binding protein, a1 microglobulin, b lactoglobulin, apolipoprotein D and odorant binding protein). The members of the lipocalin family are characterized by their ability to bind small lipophilic substances in their hydrophobic core, and thereby serve as transporters of substances. Pig NGAL may, by analogy with its homologues in other mammalian species, be released from activated neutrophils in inflammation or infection, from certain epithelial cancers, and more dramatically, from renal tubular cells in response to ischemic or nephrotoxic injury.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LCN2