Lipin 1 Antibody [Unconjugated]

Lipin 1 was detected in immersion fixed human mesenchymal stem cell-derived adipocytes using Goat Anti-Human Lipin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3885) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Lipin 1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Lipin 1
  Asp448-Leu574
  Accession # Q14693
• Specificity: Detects human Lipin 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross‑reactivity with recombinant human (rh) Lipin 2 and rhLipin 3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LPIN1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lipin 1 Antibody [Unconjugated]

• DKFZp781P1796
• EC 3.1.3.18
• EC 3.1.3.4
• EC 5.4.2.7
• KIAA0188phosphatidate phosphatase LPIN1
• Lipin 1
• Lipin-1
• LPIN1
• PAP1

Background

Lipin 1 is a 100 kDa phosphatidate phosphatase. Mammalian Lipin 1 has been identified as an enzyme that regulates fat metabolism. There is one human Lipin-1. In mice, two Lipin-1 isoforms have been reported, alpha and beta. Lipin 1 alpha is a nuclear protein and is 96% and 98 % identical to human Lipin 1 and rat Lipin 1 alpha , respectively. Lipin 1 beta is cytoplasmic.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lipin 1 Antibody (AF3885)(5)

Publications using AF3885

• M Chae, ED Son, IH Bae, EG Cho, HJ Kim, JY Jung UVB-dependent inhibition of lipin-1 protects against proinflammatory responses in human keratinocytes Exp. Mol. Med., 2020-02-21;0(0):. 2020-02-21 [PMID: 32080341] (Western Blot, Human)
• T Arai, M Tanaka, N Goda HIF-1-dependent lipin1 induction prevents excessive lipid accumulation in choline-deficient diet-induced fatty liver Sci Rep, 2018-09-21;8(1):14230. 2018-09-21 [PMID: 30242180] (ChIP, Western Blot, Mouse)
• K Ishimoto, A Hayase, F Kumagai, M Kawai, H Okuno, N Hino, Y Okada, T Kawamura, T Tanaka, T Hamakubo, J Sakai, T Kodama, K Tachibana, T Doi Degradation of human Lipin-1 by BTRC E3 ubiquitin ligase Biochem. Biophys. Res. Commun., 2017-05-05;0(0):. 2017-05-05 [PMID: 28483528] (Western Blot, Human)
• Obama T, Nagaoka S, Akagi K, Kato R, Horiuchi N, Horai Y, Aiuchi T, Arata S, Yamaguchi T, Watanabe M, Itabe H Dietary cholesterol reduces plasma triacylglycerol in apolipoprotein e-null mice: suppression of lipin-1 and -2 in the glycerol-3-phosphate pathway. PLoS ONE, 2011-08-09;6(8):e22917. 2011-08-09 [PMID: 21857965] (Western Blot, Human, Mouse)
• Ishimoto K, Nakamura H, Tachibana K, Yamasaki D, Ota A, Hirano K, Tanaka T, Hamakubo T, Sakai J, Kodama T, Doi T Sterol-mediated regulation of human lipin 1 gene expression in hepatoblastoma cells. J. Biol. Chem., 2009-06-24;284(33):22195-205. 2009-06-24 [PMID: 19553673] (Western Blot, Human)

Bioinformatics

• Gene Symbol: LPIN1
• Uniprot:
  • Q14693()