LIN-28A Antibody

Images

 
Western Blot: LIN-28A Antibody [NBP1-49537] - Analysis of LIN28 in NTERA-2 cell lysate.
Immunocytochemistry/ Immunofluorescence: LIN-28A Antibody [NBP1-49537] - Analysis of LIN28 in NTERA-2 cells using NBP1-49537. Nuclei (Blue) are counterstained using Hoechst 33258.
Immunohistochemistry: LIN-28A Antibody [NBP1-49537] - Analysis of LIN28 in E17.5 mouse embryo.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

LIN-28A Antibody Summary

Immunogen
Partial recombinant human Lin28 protein expressed in E. coli. [Swiss-Prot# Q9H9Z2]
Localization
Cytoplasm. Nucleus - nucleolus. Notes: Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes. Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules.
Marker
Undifferentiated human embryonic stem cell Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LIN28A
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 10-15 ug/ml
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Paraffin 1:1000
Application Notes
This Lin28 antibody is useful for IHC-P, ICC/IF and Western blot.
Positive Control
NTERA-2 Lysate (NBP1-44968)
Publications
Read Publications using
NBP1-49537 in the following applications:

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for LIN-28A Antibody

  • CSDD1
  • CSDD1lin-28A
  • FLJ12457
  • lin-28 homolog (C. elegans)
  • lin-28 homolog A (C. elegans)
  • LIN28
  • LIN-28
  • LIN28A
  • LIN-28A
  • LIN28RNA-binding protein LIN-28
  • Tex17
  • ZCCHC1
  • ZCCHC1protein lin-28 homolog A
  • Zinc finger CCHC domain-containing protein 1
  • zinc finger, CCHC domain containing 1

Background

Lin28 acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Lin28 is a marker of undifferentiated human embryonic stem cells and has been used to enhance the efficiency of the formation of induced pluripotent stem (iPS) cells from human fibroblasts. Lin28 has also been shown to bind to the let-7 pre-miRNA and block production of the mature let-7 microRNA in mouse embryonic stem cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LIN-28A Antibody (NBP1-49537)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(2)
WB
(1)
All Applications
Filter By Species
Human
(1)
All Species

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for LIN-28A Antibody (NBP1-49537) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol LIN28A
Entrez
Uniprot