| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3757-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-49537SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77383-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-27034 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21320-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21298-25ul | |||
![Western Blot: LIN-28A Antibody [NBP1-49537] - Hypoxia promoted an increase in the expression of SOX-2, OCT-4, KLF-4, Nanog, Lin-28A and CD133. The RT-qPCR analysis showed an upregulation in the expression of Lin-28A in a time-dependent manner in sorted A549 cells under hypoxic conditions (*P<0.05). (b) Western blot analysis showed that the transcription factors and stem cell markers were not expressed under normoxia. However, there was an increase in the expression of SOX-2 (MAB2018), OCT-4 (MAB1759), KLF-4 (AF3640), Nanog (AF1997), Lin-28A and CD133 in a time-dependent manner after hypoxia treatment in sorted A549 cells. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddiscovery2016105) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-Western-Blot-NBP1-49537-img0013.jpg "<b>Biological Strategies Validation. </b>Western Blot: LIN-28A Antibody [NBP1-49537] - Hypoxia promoted an increase in the expression of SOX-2, OCT-4, KLF-4, Nanog, Lin-28A and CD133. The RT-qPCR analysis showed an upregulation in the expression of Lin-28A in a time-dependent manner in sorted A549 cells under hypoxic conditions (*P<0.05). (b) Western blot analysis showed that the transcription factors and stem cell markers were not expressed under normoxia. However, there was an increase in the expression of SOX-2 (MAB2018), OCT-4 (MAB1759), KLF-4 (AF3640), Nanog (AF1997), Lin-28A and CD133 in a time-dependent manner after hypoxia treatment in sorted A549 cells. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddiscovery2016105) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: LIN-28A Antibody [NBP1-49537] - (e) Immunofluorescence staining showed that A549 CD133-negative cells highly expressed Lin-28A after 48 h of 1% O2 exposure. (k) Immunofluorescence staining showed that HepG2 CD133-negative cells highly expressed Lin-28A after 48 h of 1% O2 exposure.Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddiscovery2016105) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49537-img0015.jpg)
![Immunohistochemistry: LIN-28A Antibody [NBP1-49537] - Analysis of LIN28 in E17.5 mouse embryo.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-Immunohistochemistry-NBP1-49537-img0011.jpg)
![Western Blot: LIN-28A Antibody [NBP1-49537] - Analysis of LIN28 in NTERA-2 cell lysate.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-Western-Blot-NBP1-49537-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: LIN-28A Antibody [NBP1-49537] - Analysis of LIN28 in NTERA-2 cells using NBP1-49537. Nuclei (Blue) are counterstained using Hoechst 33258.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49537-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: LIN-28A Antibody [NBP1-49537] - Immunofluorescence staining showed that the newly formed GL261 spheres highly expressed Lin-28A. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddiscovery2016105) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49537-img0014.jpg)
![Western Blot: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of 75 ug of various whole cell lysates and 10 ul of PageRuler Prestained Protein Ladder onto a 4-20% Tris-HCl polyacrylamide gel.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Western-Blot-NBP2-22481-img0001.jpg "Western Blot: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of 75 ug of various whole cell lysates and 10 ul of PageRuler Prestained Protein Ladder onto a 4-20% Tris-HCl polyacrylamide gel.")
![Immunocytochemistry/Immunofluorescence: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of LIN28 in NCCIT and HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a LIN28 monoclonal antibody at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Immunocytochemistry-Immunofluorescence-NBP2-22481-img0005.jpg)
![Immunohistochemistry-Paraffin: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis showing staining in the cytoplasm of human seminoma (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Immunohistochemistry-Paraffin-NBP2-22481-img0015.jpg)
![Flow Cytometry: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of Lin28 (blue histogram) on H9 embryonic stem cells. To generate single cells suspensions, colonies were treated with TrypLE cell dissociation enzyme for 5 minutes at 37C. Cells were incubated with a Lin28 monoclonal antibody or mouse IgG (green histogram) at a dilution of 1:100 for 1 hour on ice, washed with PBS + 5% fetal calf serum (FACS buffer), and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:200 for 30 minutes on ice. Cells were washed with cold FACS buffer, resuspended in 500ul of FACS buffer containing 10ul of 4% paraformaldehyde.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Flow-Cytometry-NBP2-22481-img0013.jpg)
![Chromatin Immunoprecipitation: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis performed using cross-linked chromatin from rat hepatoma cells treated with insulin. IP performed using a multiplex microplate Matrix ChIP assay of LIN28 monoclonal antibody. Chromatin aliquots from cells were used per ChIP pull-down. Quantitative PCR data done in quadruplicate using 1ul of DNA in 2ul SYBR real-time PCR reactions containing primers to amplify -15kb upstream of Egr1 or exon-1 or exon-2-3 of Egr1. Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM. A schematic representation of the rat Egr-1 locus is shown; oxes represent exons (black boxes = translated, white boxes = untranslated), the zigzag line represents an intron, and the straight line represents upstream sequence. Regions amplified by Egr-1 primers are represented by black bars. Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Chromatin-Immunoprecipitation-ChIP-NBP2-22481-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of Lin28 (green) in H9 embryonic stem cells grown for a few days on Matrigel-coated chamber slides. Cells fixed in 4% paraformaldehyde were permeabilized with 0.1% Triton X-100 for 15 minutes at room temperature. Cells were probed with a Lin28 monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBST, and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:100 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI and cells were analyzed by fluorescence microscopy at 20X magnification.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Immunocytochemistry-Immunofluorescence-NBP2-22481-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of Lin28 (green) in HEL 11.4 induced IPS cells grown for a few days on Matrigel-coated chamber slides. Cells fixed in 4% paraformaldehyde were permeabilized with 0.1% Triton X-100 for 15 minutes at room temperature. Cells were probed with a Lin28 monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBST, and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:100 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI and cells were analyzed by fluorescence microscopy at 20X magnification.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Immunocytochemistry-Immunofluorescence-NBP2-22481-img0004.jpg)
![Immunohistochemistry-Paraffin: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis showing staining in the nucleus and cytoplasm of mouse testis tissue (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Immunohistochemistry-Paraffin-NBP2-22481-img0014.jpg)
![Flow Cytometry: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of Lin28 (blue histogram) on HEL 11.4 induced IPS cells. To generate single cells suspensions, colonies were treated with TrypLE cell dissociation enzyme for 5 minutes at 37C. Cells were incubated with a Lin28 monoclonal antibody or mouse IgG (green histogram) at a dilution of 1:100 for 1 hour on ice, washed with PBS + 5% fetal calf serum (FACS buffer), and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:200 for 30 minutes on ice. Cells were washed with cold FACS buffer, resuspended in 500ul of FACS buffer containing 10ul of 4% paraformaldehyde.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Flow-Cytometry-NBP2-22481-img0012.jpg)
![Immunoprecipitation: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of LIN28 was performed. Antigen-antibody complexes were formed by incubating 700ug of lysate with 5 ug of an LIN28 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50 ul Protein A/G Agarose was loaded as a positive control for detection. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a LIN28 monoclonal antibody at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-blot IP Detection Reagent at a dilution of 1:1000 for at least 1 hour.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Immunoprecipitation-NBP2-22481-img0002.jpg)
![Western Blot: LIN-28A Antibody (717) [NBP2-43672] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with LIN-28A antibody diluted at 1:500.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-717-Western-Blot-NBP2-43672-img0008.jpg "Western Blot: LIN-28A Antibody (717) [NBP2-43672] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with LIN-28A antibody diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: LIN-28A Antibody (717) [NBP2-43672] - Analysis of human embryonic stem cell were fixed in 4% paraformaldehyde for 15 min. Green: LIN28 protein stained by LIN28 antibody diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-717-Immunocytochemistry-Immunofluorescence-NBP2-43672-img0002.jpg)
![Immunohistochemistry: LIN-28A Antibody (717) [NBP2-43672] - Analysis of U87 xenograft. Lin28A antibody dilution: 1:100.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-717-Immunohistochemistry-NBP2-43672-img0007.jpg)
![Flow Cytometry: LIN-28A Antibody (717) [NBP2-43672] - Analysis of Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Lin28A antibody [717] dilution: 1:50](http://images.novusbio.com/fullsize/LIN-28A-Antibody-717-Flow-Cytometry-NBP2-43672-img0001.jpg)
![Western Blot: LIN-28A Antibody (717) [NBP2-43672] - Analysis of 30 ug whole cell lysate. A: Non-transfected HeLa lysates B: LIN28 transfected HeLa lysates 12% SDS PAGE diluted at 1:1000.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-717-Western-Blot-NBP2-43672-img0004.jpg)
![Western Blot: LIN-28A Antibody (717) [NBP2-43672] - Analysis of 30 ug whole cell lysate. A: NT2D1 12% SDS PAGE diluted at 1:1000. .](http://images.novusbio.com/fullsize/LIN-28A-Antibody-717-Western-Blot-NBP2-43672-img0005.jpg)
![Western Blot: LIN-28A Antibody (717) [NBP2-43672] - Analysis of A. 30 ug human ESC whole cell lysate/extract 12 % SDS-PAGE Lin28A antibody [717] dilution: 1:1000](http://images.novusbio.com/fullsize/LIN-28A-Antibody-717-Western-Blot-NBP2-43672-img0006.jpg)
![Western Blot: LIN-28A Antibody (1086) [NBP2-43673] - Analysis of A. 30 ug human ESC whole cell lysate/extract 12 % SDS-PAGE Lin28A antibody [717] dilution: 1:1000.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-1086-Western-Blot-NBP2-43673-img0006.jpg "Western Blot: LIN-28A Antibody (1086) [NBP2-43673] - Analysis of A. 30 ug human ESC whole cell lysate/extract 12 % SDS-PAGE Lin28A antibody [717] dilution: 1:1000.")
![Immunocytochemistry/Immunofluorescence: LIN-28A Antibody (1086) [NBP2-43673] - Analysis of human embryonic stem cell were fixed in 4% paraformaldehyde for 15 min. Green: LIN28 protein stained by LIN28 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-1086-Immunocytochemistry-Immunofluorescence-NBP2-43673-img0002.jpg)
![Immunohistochemistry-Paraffin: LIN-28A Antibody (1086) [NBP2-43673] - Analysis of U87 xenograft. Lin28A antibody dilution: 1:100.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-1086-Immunohistochemistry-Paraffin-NBP2-43673-img0003.jpg)
![Flow Cytometry: LIN-28A Antibody (1086) [NBP2-43673] - Analysis of Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: Lin28A antibody [1086] dilution: 1:50](http://images.novusbio.com/fullsize/LIN-28A-Antibody-1086-Flow-Cytometry-NBP2-43673-img0001.jpg)
![Western Blot: LIN-28A Antibody (1086) [NBP2-43673] - Analysis of 30 ug whole cell lysate. A: Non-transfected HeLa lysates B: LIN28 transfected HeLa lysates 12% SDS PAGE diluted at 1:1000. .](http://images.novusbio.com/fullsize/LIN-28A-Antibody-1086-Western-Blot-NBP2-43673-img0004.jpg)
![Western Blot: LIN-28A Antibody (1086) [NBP2-43673] - Analysis of 30 ug whole cell lysate. A: NT2D1 12% SDS PAGE diluted at 1:1000.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-1086-Western-Blot-NBP2-43673-img0005.jpg)
![Western Blot: LIN-28A Antibody [NBP2-19355] - Sample (20 ug of whole cell lysate) A: mouse ESC 12% SDS PAGE gel, diluted at 1:3000.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-Western-Blot-NBP2-19355-img0008.jpg "Western Blot: LIN-28A Antibody [NBP2-19355] - Sample (20 ug of whole cell lysate) A: mouse ESC 12% SDS PAGE gel, diluted at 1:3000.")
![Immunohistochemistry-Paraffin: LIN-28A Antibody [NBP2-19355] - Immunohistochemical analysis of paraffin-embedded Gastric ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-Immunohistochemistry-Paraffin-NBP2-19355-img0006.jpg)
![Flow Cytometry: LIN-28A Antibody [NBP2-19355] - Human embryonic stem cells, staining with LIN28 antibody at 1:100 dilution (purple) or rabbit IgG (black).](http://images.novusbio.com/fullsize/LIN-28A-Antibody-Flow-Cytometry-NBP2-19355-img0005.jpg)
![Western Blot: LIN-28A Antibody [NBP2-19355] - Sample (20 ug of whole cell lysate) A: hESC 12% SDS PAGE NBP2-19355 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19355_rabbit-polyclonal-lin-28a-antibody-910202420134044.jpg)
![Western Blot: LIN-28A Antibody (2S1I10) [NBP3-16843] - Western blot analysis of extracts of various cell lines, using LIN-28A antibody (NBP3-16843) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-2S1I10-Western-Blot-NBP3-16843-img0004.jpg "Western Blot: LIN-28A Antibody (2S1I10) [NBP3-16843] - Western blot analysis of extracts of various cell lines, using LIN-28A antibody (NBP3-16843) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunohistochemistry: LIN-28A Antibody (2S1I10) [NBP3-16843] - Immunofluorescence analysis of mouse testis using LIN-28A Rabbit mAb (NBP3-16843) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-2S1I10-Immunohistochemistry-NBP3-16843-img0003.jpg)
![Immunohistochemistry: LIN-28A Antibody (2S1I10) [NBP3-16843] - Immunofluorescence analysis of rat testis using LIN-28A Rabbit mAb (NBP3-16843) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-2S1I10-Immunohistochemistry-NBP3-16843-img0001.jpg)
![Immunohistochemistry: LIN-28A Antibody (2S1I10) [NBP3-16843] - Immunofluorescence analysis of human ovary using LIN-28A Rabbit mAb (NBP3-16843) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-2S1I10-Immunohistochemistry-NBP3-16843-img0002.jpg)
![Western Blot: LIN-28A Antibody [NBP1-77383] - Analysis of LIN28 in Raji cell lysate with LIN28 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-Western-Blot-NBP1-77383-img0004.jpg "Western Blot: LIN-28A Antibody [NBP1-77383] - Analysis of LIN28 in Raji cell lysate with LIN28 antibody at (A) 1 and (B) 2 ug/mL.")
![Immunohistochemistry: LIN-28A Antibody - BSA Free [NBP1-77383] - Immunohistochemistry of KLF4 in human spleen tissue with KLF4 antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp1-77383_rabbit-polyclonal-lin-28a-antibody-2711202419353888.jpg)
![Immunocytochemistry/ Immunofluorescence: LIN-28A Antibody - BSA Free [NBP1-77383] - Immunofluorescence of LIN28 in human spleen tissue with LIN28 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77383_rabbit-polyclonal-lin-28a-antibody-2711202419241328.jpg)
![Western Blot: Lin28 Overexpression Lysate (Adult Normal) [NBL1-12541] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Lin28.](http://images.novusbio.com/fullsize/Lin28-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12541-img0002.jpg "Western Blot: Lin28 Overexpression Lysate (Adult Normal) [NBL1-12541] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Lin28.")