CACNA1E Antibody (N493/22) - Chimeric - BSA Free

Western blotting of rat brain membrane with NBP3-52364 recognizing endogenous CACNA1E at ~250 kDa. This antibody was detected with anti-chicken-HRP.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: N493/22
• Clonality: Monoclonal
• Host: Chicken
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CACNA1E Antibody (N493/22) - Chimeric - BSA Free Summary

• Description: This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N493/22 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N493/22 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in HEK293 cells and then affinity purified from the cell culture media.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein amino acids 956-1033 (ID I-II cytoplasmic loop) of mouse CACNA1E (Uniprot#: Q61290) produced recombinantly in E.Coli.
• Isotype: IgY
• Clonality: Monoclonal
• Host: Chicken
• Gene: CACNA1E
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 257 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1X PBS (pH 7.4)
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Affinity purified

Alternate Names for CACNA1E Antibody (N493/22) - Chimeric - BSA Free

• BII
• Brain calcium channel II
• CACH6
• CACH6calcium channel, R type, alpha-1 polypeptide
• CACNA1E
• CACNL1A6
• CACNL1A6calcium channel, voltage-dependent, alpha 1E subunit
• calcium channel, voltage-dependent, R type, alpha 1E subunit
• Cav2.3
• L type, alpha-1 polypeptide
• L type, alpha-1 polypeptide, isoform 6
• voltage-dependent calcium channel alpha 1E subunit
• voltage-dependent R-type calcium channel subunit alpha-1E
• voltage-gated calcium channel alpha 1E subunit
• Voltage-gated calcium channel subunit alpha Cav2.3

Background

Function: Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1E gives rise to R-type calcium currents. R-type calcium channels belong to the 'high-voltage activated' (HVA) group and are blocked by nickel, and partially by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to dihydropyridines (DHP), omega-conotoxin-GVIA (omega-CTx-GVIA), and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels contaning alpha-1E subunit could be involved in the modulation of firing patterns of neurons which is important for information processing.; Subcellular location: Membrane; Multi-pass membrane protein.; Tissue specificity: Expressed in neuronal tissues and in kidney.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CACNA1E