LIMPII/SR-B2 Antibody (SR2211) - BSA Free

Western Blot: LIMPII/SR-B2 Antibody (SR2211) [NBP3-22186] - Western blot analysis using NBP3-22186 in (1) HeLa cell lysate; (2) Mouse liver lysate.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC, IP
• Clone: SR2211
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

LIMPII/SR-B2 Antibody (SR2211) - BSA Free Summary

• Description: Novus Biologicals Rabbit LIMPII/SR-B2 Antibody (SR2211) - BSA Free (NBP3-22186) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human LIMPII/SR-B2 (Uniprot #: Q14108)
• Specificity: Detects endogenous levels of total LIMPII/SR-B2
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SCARB2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50
  • Immunohistochemistry 1:50-1:200
  • Immunoprecipitation 1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 77 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for LIMPII/SR-B2 Antibody (SR2211) - BSA Free

• AMRF
• CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 2(lysosomal integral membrane protein II)
• CD36 antigen
• CD36L2
• CD36L2LIMP II
• HLGP85
• LGP85
• LIMP-2,85 kDa lysosomal sialoglycoprotein scavenger receptor class B, member 2
• LIMPII
• LIMPIICD36 antigen-like 2
• LPG85
• lysosome membrane protein 2
• Lysosome membrane protein II
• SCARB2
• Scavenger receptor class B member 2,85 kDa lysosomal membrane sialoglycoprotein
• scavenger receptor class B, member 2
• SRB2
• SR-B2
• SR-BII

Background

High density lipoproteins (HDLs) play a critical role in cholesterol metabolism and their plasma concentrations are inversely correlated with risk for atherosclerosis. SR-BI and SR-BII (previously known as SR-BI.2) are the alternatively spliced products of a single gene. SR-BII differs from SR-BI in that the encoded c-terminal cytoplasmic domain is almost completely different. SR-BII binds HDLs and mediates selective uptake of HDL cholesteryl ester, but with an approximately 4-fold lower efficiency than SR-BI. Nuclease protection assays show SR-BII to be abundant in mouse tissues expressing SR-BI, with SR-BII expression found in liver, adrenal glands, and testes. Although the role of SR-BII is not completely clear, research suggests that it may be a functional HDL receptor. In addition, SR-BII mRNA results from the alternative splicing of SR-BI precursor transcripts with the SR-BII isoform mediating selective transfer of lipid between HDL and cells. The relative expression and functional activities of these two isoforms create a potential means of regulating selective lipid transfer between HDL and cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SCARB2