LIGHT/TNFSF14 Antibody

Western Blot: LIGHT/TNFSF14 Antibody [NBP1-51981] - Staining of human cerebellum (A) and frontal cortex (B) lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: LIGHT/TNFSF14 Antibody [NBP1-51981] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

LIGHT/TNFSF14 Antibody Summary

• Immunogen: Peptide with sequence C-HEVNPAAHLTGANS corresponding to internal region according to NP_003798.2, NP_742011.1.
• Epitope: C-HEVNPAAHLTGANS
• Specificity: This antibody is expected to recognize both reported isoforms (NP_003798.2; NP_742011.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TNFSF14
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.03 - 0.1 ug/mL
• Application Notes: WB: Approx. 22 kDa band observed in human brain (cerebellum and frontal cortex) lysates (calculated MW of 22.4 kDa band according to NP_742011.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for LIGHT/TNFSF14 Antibody

• CD258 antigen
• CD258
• delta transmembrane LIGHT
• Herpes virus entry mediator ligand
• Herpesvirus entry mediator ligand
• HVEM-L
• HVEMLherpesvirus entry mediator-ligand
• ligand for herpesvirus entry mediator
• LIGHT
• LIGHTherpesvirus entry mediator A
• LTg
• TNFSF14
• TR2
• tumor necrosis factor (ligand) superfamily, member 14
• tumor necrosis factor ligand superfamily member 14
• tumor necrosis factor receptor-like 2
• tumor necrosis factor superfamily member LIGHT

Background

The T5-39 antibody recognizes human LIGHT (CD258) also known as ligand for herpesvirus entry mediator, tumor necrosis factor receptor-like-2 ligand, TR2 ligand, and tumor necrosis factor ligand superfamily member 14 (TNFSF14). LIGHT is a type 2 transmembrane protein, and a member of the tumor necrosis factor ligand superfamily. LIGHT can be proteolytically cleaved and two isofoms have been shown to be produced by alternative splicing. The apparent molecular weight of LIGHT us approximately 30 kD. LIGHT expression has been observed in spleen, brain and kidney; expression is upregulated on activated T cells (particularly CD8+ cells). LIGHT stimulates proliferation of T cells and inhibits growth of some tumor cells. This protein has been shown to play a role in intestinal inflammation and contribute to IgA nephropathy. LIGHT is a ligand for HVEM, and LTbetaR and has also been shown to bind DcR3 and modulate function. In addition, LIGHT has been shown to interact with other proteins including TRAF2, TRAF3, and DIABLO. The T5-39 antibody has been shown to be useful for flow cytometry and ELISA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LIGHT/TNFSF14 Antibody (NBP1-51981)(1)

Publication using NBP1-51981

• Cohavy O, Zhou J, Ware CF, Targan SR LIGHT is constitutively expressed on T and NK cells in the human gut and can be induced by CD2-mediated signaling J Immunol 2005-01-15 [PMID: 15634882]

Bioinformatics

• Gene Symbol: TNFSF14
• Entrez:
  • 8740(Human)
• Uniprot:
  • NP_003798.2(Human)