LIGHT/TNFSF14 Antibody [Unconjugated]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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LIGHT/TNFSF14 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human LIGHT/TNFSF14
Asp74-Val240
Accession # O43557
Specificity
Detects human LIGHT/TNFSF14 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human (rh) APRIL is observed and less than 1% cross-reactivity with rhFas Ligand, rhTRAIL, and rhTNF-alpha is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TNFSF14
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • ELISA
  • Immunohistochemistry 3-15 ug/mL
  • Neutralization 5-20 ng/mL
Publications
Read Publications using
AF664 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LIGHT/TNFSF14 Antibody [Unconjugated]

  • CD258 antigen
  • CD258
  • delta transmembrane LIGHT
  • Herpes virus entry mediator ligand
  • Herpesvirus entry mediator ligand
  • HVEM-L
  • HVEMLherpesvirus entry mediator-ligand
  • ligand for herpesvirus entry mediator
  • LIGHT
  • LIGHTherpesvirus entry mediator A
  • LTg
  • TNFSF14
  • TR2
  • tumor necrosis factor (ligand) superfamily, member 14
  • tumor necrosis factor ligand superfamily member 14
  • tumor necrosis factor receptor-like 2
  • tumor necrosis factor superfamily member LIGHT

Background

Human LIGHT is a type II membrane protein that is a member of the TNF superfamily. LIGHT is an acronym which stands for "is homologous to lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes". LIGHT has also been called HVEM-L and LT-gamma. Under the new TNF nomenclature, it is called TNFSF14. LIGHT is a 240 amino acid (aa) protein that contains a 37 aa cytoplasmic domain, a 22 aa transmembrane region, and a 181 aa extracellular domain. Similar to other TNF ligand family members, LIGHT is predicted to assemble as a homotrimer. LIGHT is produced by activated T cells and was first identified by its ability to compete with HSV glycoprotein D for HVEM binding. LIGHT has also been shown to bind to the lymphotoxin beta receptor (LT beta R) and the decoy receptor (DcR3/TR6). LIGHT overexpression in tumor cells induces apoptosis, which can be enhanced by IFN-gamma. The full roles of LIGHT remain to be elucidated.

  1. Mauri, D.N. et al. (1998) Immunity 8:21.
  2. Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142.
  3. Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548.
  4. Yu, K-Y. et al. (1999) J. Biol. Chem. 274:13733.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LIGHT/TNFSF14 Antibody (AF664)(3)

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Secondary Antibodies

 

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Other Available Formats

Biotin BAF664

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Bioinformatics

Gene Symbol TNFSF14
Uniprot