LIGHT/TNFSF14 Antibody [Unconjugated]

• Reactivity: Hu
• Applications: WB, ELISA, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

LIGHT/TNFSF14 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human LIGHT/TNFSF14
  Asp74-Val240
  Accession # O43557
• Specificity: Detects human LIGHT/TNFSF14 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human (rh) APRIL is observed and less than 1% cross-reactivity with rhFas Ligand, rhTRAIL, and rhTNF-alpha is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TNFSF14
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
  • ELISA
  • Immunohistochemistry 3-15 ug/mL
  • Neutralization 5-20 ng/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LIGHT/TNFSF14 Antibody [Unconjugated]

• CD258 antigen
• CD258
• delta transmembrane LIGHT
• Herpes virus entry mediator ligand
• Herpesvirus entry mediator ligand
• HVEM-L
• HVEMLherpesvirus entry mediator-ligand
• ligand for herpesvirus entry mediator
• LIGHT
• LIGHTherpesvirus entry mediator A
• LTg
• TNFSF14
• TR2
• tumor necrosis factor (ligand) superfamily, member 14
• tumor necrosis factor ligand superfamily member 14
• tumor necrosis factor receptor-like 2
• tumor necrosis factor superfamily member LIGHT

Background

Human LIGHT is a type II membrane protein that is a member of the TNF superfamily. LIGHT is an acronym which stands for "is homologous to lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes". LIGHT has also been called HVEM-L and LT-gamma. Under the new TNF nomenclature, it is called TNFSF14. LIGHT is a 240 amino acid (aa) protein that contains a 37 aa cytoplasmic domain, a 22 aa transmembrane region, and a 181 aa extracellular domain. Similar to other TNF ligand family members, LIGHT is predicted to assemble as a homotrimer. LIGHT is produced by activated T cells and was first identified by its ability to compete with HSV glycoprotein D for HVEM binding. LIGHT has also been shown to bind to the lymphotoxin beta receptor (LT beta R) and the decoy receptor (DcR3/TR6). LIGHT overexpression in tumor cells induces apoptosis, which can be enhanced by IFN-gamma. The full roles of LIGHT remain to be elucidated.

1. Mauri, D.N. et al. (1998) Immunity 8:21.
2. Zhai, Y. et al. (1998) J. Clin. Invest. 102:1142.
3. Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548.
4. Yu, K-Y. et al. (1999) J. Biol. Chem. 274:13733.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for LIGHT/TNFSF14 Antibody (AF664)(3)

Publications using AF664

• Holmes T, Wilson E, Black E, Benest A, Vaz C, Tan B, Tanavde V, Cook G Licensed human natural killer cells aid dendritic cell maturation via TNFSF14/LIGHT. Proc Natl Acad Sci U S A, 2014;111(52):E5688-96. 2014 [PMID: 25512551] (Neutralization, Human)
• Otterdal K, Smith C, Oie E, Pedersen TM, Yndestad A, Stang E, Endresen K, Solum NO, Aukrust P, Damas JK Platelet-derived LIGHT induces inflammatory responses in endothelial cells and monocytes. Blood, 2006;108(3):928-35. 2006 [PMID: 16861346] (Neutralization, Human)
• Le Bras B, Barallobre MJ, Homman-Ludiye J, Ny A, Wyns S, Tammela T, Haiko P, Karkkainen MJ, Yuan L, Muriel MP, Chatzopoulou E, Breant C, Zalc B, Carmeliet P, Alitalo K, Eichmann A, Thomas JL VEGF-C is a trophic factor for neural progenitors in the vertebrate embryonic brain. Nat. Neurosci., 2006;9(3):340-8. 2006 [PMID: 16462734] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: TNFSF14
• Uniprot:
  • O43557()