Canine LIF ELISA Kit (Colorimetric)

• Reactivity: Ca
• Applications: ELISA
• Suitable Sample Type: Tissue homogenates, Cell lysates and other biological fluids
• Standard Curve Range: 31.2 - 2000 pg/mL (example only; lot dependent)
• Sensitivity: 12.1 pg/mL (example only; lot dependent)

Canine LIF ELISA Kit (Colorimetric) Summary

• Description: Assay time: 3 hours
• Standard Curve Range: 31.2 - 2000 pg/mL (example only; lot dependent)
• Sensitivity: 12.1 pg/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12 (example only; lot dependent)
• Intra-Assay: %CV < 10 (example only; lot dependent)
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: LIF

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Canine LIF ELISA Kit (Colorimetric)

• CDF
• D Factor
• DIA
• differentiation inhibitory activity
• differentiation stimulating factor
• Differentiation-stimulating factor
• Emfilermin
• HILDA
• HILDAcholinergic differentiation factor
• leukemia inhibitory factor (cholinergic differentiation factor)
• leukemia inhibitory factor
• LIF
• Melanoma-derived LPL inhibitor
• MLPLI

Background

LIF (leukemia inhibitory factor, myeloid leukemia inhibitory factor) a pleiotropic lymphoid factor was initially described as a factor that inhibits the proliferation of myeloid leukemia cells and induces their differentiation into macrophages. Other activities of LIF include inhibition of adipogenesis, cholinergic neuron differentiation and bone metabolism. Human and mouse LIF share 78% homology and activities of LIF are not species-specific. Human LIF is active on mouse cells, but murine LIF is not active on human cells. Benefits of NovActive® Proteins

Fully Active

-Fully folded naturally by eukaryotic source

-Comparable for eukaryotic glycosylation

BioRisk Free

-animal-free

-serum-free

-endotoxin-free

-infection reagent-free

-low in proteolyic activity

-low in pyrogenic and pro-inflammatory activity

Just reconstitute and add to your cell culture.

Have FDA G.R.A.S. (generally recognized as safe) approval.

Advantages of barley endosperm Folding -Proficient protein machinery, with eukaryotic folding, allows for native formation of the protein and long term protection and storage. Environment -A biochemically inert environment, void of endotoxins, low protease activity and secondary metabolite content, and a simple protein profile, aid in downstream processing in barley. FDA G.R.A.S status -Barley is recognized by the FDA as having G.R.A.S (generally recognized as safe) status.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for LIF ELISA Kit (NBP3-42859). (Showing 1 - 1 of 1 FAQ).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: LIF