LIF Antibody (9808) [Alexa Fluor® 594]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
9808
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 594
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

LIF Antibody (9808) [Alexa Fluor® 594] Summary

Immunogen
E. coli-derived recombinant human LIF
Specificity
Detects human LIF in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse LIF, recombinant human (rh) CLC, rhCNTF, rhCardiotrophin-1, rhIL-6, rhIL-11, or rhOSM is observed.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
LIF
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Intracellular Staining by Flow Cytometry 0.25-1 ug/10^6 cells
Application Notes
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Buffer
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LIF Antibody (9808) [Alexa Fluor® 594]

  • CDF
  • D factor
  • DIA
  • differentiation inhibitory activity
  • differentiation stimulating factor
  • Differentiation-stimulating factor
  • Emfilermin
  • HILDAcholinergic differentiation factor
  • leukemia inhibitory factor (cholinergic differentiation factor)
  • leukemia inhibitory factor
  • LIF
  • Melanoma-derived LPL inhibitor
  • MLPLI

Background

LIF is a 36‑67 kDa highly glycosylated polypeptide (1, 2) produced by a variety of cells including T cells (3), monocytes (4), fibroblasts (5), osteoblasts (6) and mast cells (7). Consistent with its many synonyms, LIF exhibits a broad spectrum of effects on both hematopoietic and nonhematopoietic cells. For example, LIF inhibits the differentiation of embryonic stem cells (8), up regulates the synthesis of acute phase proteins in hepatocytes (9), down regulates lipoprotein lipase activity in adipocytes (10), and preferentially induces a cholinergic phenotype in sympathetic neurons (11). The receptor for LIF (LIF R) has been isolated and found to be a 190 kDa type I transmembrane glycoprotein (12). Although this molecule binds LIF, the resultant LIF-LIF R complex is not sufficient to transduce an intracellular signal. This capability is provided by a 130 kDa signal transducing subunit (gp130) that is common to the functional receptors for IL-6, IL-11, CNTF, and Oncostatin M (13, 14). Since gp130 is a ubiquitously expressed membrane protein, the presence of LIF R (membrane-bound or soluble form) ultimately determines the cell’s responsiveness to LIF. Cells known to express LIF R include osteoblasts (6), hepatocytes (15), macrophages (15), neurons (5), and megakaryocytes (16). Human and mouse LIF exhibit 78% sequence homology, and human LIF is biologically active on mouse cells.
  1. Van-Vlasselaev, P. et al. (1992) Prog. Growth Factor Res. 4:337.
  2. Gough, N.M. (1992) Growth Factors 7:175.
  3. Anegon, N.M, I. et al. (1991) J. Immunol.147:3973.
  4. Gillett, N.A. et al. (1993) Growth Factors 9:301.
  5. Banner, L. R. and P.H. Patterson (1994) Proc. Natl. Acad. Sci. 91:7109.
  6. Allen, E.H. et al. (1990) J. Cell. Physiol. 145:110.
  7. Lorenzo, J.A. et al. (1994) Clin.Immunol. Immunopathol. 70:260.
  8. Williams, R.L. et al. (1988) Nature 336:684.
  9. Baumann, H. et al. (1989) J. Immunol. 143:1163.
  10. Marshall, M.K. et al. (1994) Endocrinology 135:1412.
  11. Ludham, W.H. et al. (1994) Dev. Biol. 164:5283.
  12. Davis, S. et al. (1993) Science 260:18054.
  13. Gearing, D.P. et al. (1991) EMBO J. 10:28395.
  14. Gearing, D.P. et al. (1991) Science 255:1434.
  15. Hilton, D.J. and N.A. Nicola (1992) J. Biol. Chem. 267:102286.
  16. Hilton, D.J. et al. (1992) Ciba17. Foundation Symposium 167:2278.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics Tool for LIF Antibody (IC2501T)

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Bioinformatics

Gene Symbol LIF