LIF Antibody (945842) [Alexa Fluor™ Plus 680]

• Reactivity: Hu
• Clone: 945842
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 680

LIF Antibody (945842) [Alexa Fluor™ Plus 680] Summary

• Specificity: Detects human LIF in direct ELISA
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for LIF Antibody (945842) [Alexa Fluor™ Plus 680]

• CDF
• D Factor
• DIA
• differentiation inhibitory activity
• differentiation stimulating factor
• Differentiation-stimulating factor
• Emfilermin
• HILDA
• HILDAcholinergic differentiation factor
• leukemia inhibitory factor (cholinergic differentiation factor)
• leukemia inhibitory factor
• LIF
• Melanoma-derived LPL inhibitor
• MLPLI

Background

LIF is a 36‑67 kDa highly glycosylated polypeptide (1, 2) produced by a variety of cells including T cells (3), monocytes (4), fibroblasts (5), osteoblasts (6) and mast cells (7). Consistent with its many synonyms, LIF exhibits a broad spectrum of effects on both hematopoietic and nonhematopoietic cells. For example, LIF inhibits the differentiation of embryonic stem cells (8), up regulates the synthesis of acute phase proteins in hepatocytes (9), down regulates lipoprotein lipase activity in adipocytes (10), and preferentially induces a cholinergic phenotype in sympathetic neurons (11). The receptor for LIF (LIF R) has been isolated and found to be a 190 kDa type I transmembrane glycoprotein (12). Although this molecule binds LIF, the resultant LIF-LIF R complex is not sufficient to transduce an intracellular signal. This capability is provided by a 130 kDa signal transducing subunit (gp130) that is common to the functional receptors for IL-6, IL-11, CNTF, and Oncostatin M (13, 14). Since gp130 is a ubiquitously expressed membrane protein, the presence of LIF R (membrane-bound or soluble form) ultimately determines the cell’s responsiveness to LIF. Cells known to express LIF R include osteoblasts (6), hepatocytes (15), macrophages (15), neurons (5), and megakaryocytes (16). Human and mouse LIF exhibit 78% sequence homology, and human LIF is biologically active on mouse cells.

1. Van-Vlasselaev, P. et al. (1992) Prog. Growth Factor Res. 4:337.
2. Gough, N.M. (1992) Growth Factors 7:175.
3. Anegon, N.M, I. et al. (1991) J. Immunol.147:3973.
4. Gillett, N.A. et al. (1993) Growth Factors 9:301.
5. Banner, L. R. and P.H. Patterson (1994) Proc. Natl. Acad. Sci. 91:7109.
6. Allen, E.H. et al. (1990) J. Cell. Physiol. 145:110.
7. Lorenzo, J.A. et al. (1994) Clin.Immunol. Immunopathol. 70:260.
8. Williams, R.L. et al. (1988) Nature 336:684.
9. Baumann, H. et al. (1989) J. Immunol. 143:1163.
10. Marshall, M.K. et al. (1994) Endocrinology 135:1412.
11. Ludham, W.H. et al. (1994) Dev. Biol. 164:5283.
12. Davis, S. et al. (1993) Science 260:18054.
13. Gearing, D.P. et al. (1991) EMBO J. 10:28395.
14. Gearing, D.P. et al. (1991) Science 255:1434.
15. Hilton, D.J. and N.A. Nicola (1992) J. Biol. Chem. 267:102286.
16. Hilton, D.J. et al. (1992) Ciba17. Foundation Symposium 167:2278.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.