Leptin R Antibody

Western Blot: Leptin R Antibody [NB100-40796] - (1.75ug/ml) staining of Human Cerebellum (A), and (1.5ug/ml) Mouse Foetal Brain (B) and Rat Brain (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Immunohistochemistry: Leptin R Antibody [NB100-40796] - (6ug/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.

Flow Cytometry: Leptin R Antibody [NB100-40796] - Analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Control: Leptin R Antibody [NB100-40796] - Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA, Flow, IHC, Ctrl
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Leptin R Antibody Summary

• Description: Novus Biologicals Goat Leptin R Antibody (NB100-40796) is a polyclonal antibody validated for use in IHC, WB, ELISA and Flow. Anti-Leptin R Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-TQDDIEKHQSDAG corresponding to internal region according to NP_002294.2, NP_001003679.1, NP_001003680.1.
• Specificity: This antibody is expected to recognise all three reported isoforms (NP_002294.2; NP_001003679.1; NP_001003680.1).
• Predicted Species: Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: LEPR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Control
  • Flow Cytometry 10ug/ml
  • Immunohistochemistry 4-6ug/ml
  • Immunohistochemistry-Paraffin 4-6ug/ml
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 1-2ug/ml
• Application Notes: Western blot: Approx 140-150kDa band observed in Human Cerebellum, Mouse Foetal Brain and Rat Brain lysates (calculated MW of 132kDa according to Human NP_002294.2 and 130.8kDa according to Mouse NP_666258.2 and Rat NP_036728.1). This molecular weight is routinely observed by other sources. Primary incubation 1 hour at room temperature.
• Control:

  Leptin R Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Leptin R Antibody

• B219
• CD295 antigen
• CD295
• DB
• DKFZp686B1731
• huB219
• LEPR
• LEP-R
• Leptin R
• leptin receptor
• LeptinR
• OB R
• OB receptor
• OB-R
• OBRCD295

Background

Leptin receptor (OB-R) is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and LIF receptor. An OB-R transcript encodes a soluble form of the receptor. The transcript was reported to be expressed predominantly in regions of the hypothalamus previously thought to be important in body weight regulation. OB-R binds leptin with high affinity and is a potent leptin antagonist. Human OB-R encodes a 1 165 amino acid residue precursor protein with a 22 amino acid residue signal peptide, an 816 amino acid residue extracellular domain, a 21 amino acid residue transmembrane domain and a 303 amino acid residue cytoplasmic domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Leptin R Antibody (NB100-40796)(2)

Publications using NB100-40796

• Targa, G;Mottarlini, F;Rizzi, B;Taddini, S;Parolaro, S;Fumagalli, F;Caffino, L; Anorexia-Induced Hypoleptinemia Drives Adaptations in the JAK2/STAT3 Pathway in the Ventral and Dorsal Hippocampus of Female Rats Nutrients 2024-04-15 [PMID: 38674862]
• Snoussi K, Strosberg AD, Bouaouina N et al. Leptin and leptin receptor polymorphisms are associated with increased risk and poor prognosis of breast carcinoma. BMC Cancer 2006-02-20 [PMID: 16504019]

Bioinformatics

• Gene Symbol: LEPR
• Entrez:
  • 3953(Human)
• Uniprot:
  • NP_002294.2(Human)