LEO1 Antibody

Images

 
Western Blot: LEO1 Antibody [NB600-276] - Detection of mouse Leo1 by western blot. Samples: Whole cell lysate (15 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit ...read more
Immunohistochemistry: LEO1 Antibody [NB600-276] - Detection of mouse Leo1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-Leo1 (NB600-276). Detection: ...read more
Western Blot: LEO1 Antibody [NB600-276] - Detection of human Leo1 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, MCF-7, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified ...read more
Immunohistochemistry: LEO1 Antibody [NB600-276] - Sample: FFPE section of human stomach carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-LEO1 used at a dilution of 1:5,000 (0.2ug/ml) ...read more
Immunoprecipitation: LEO1 Antibody [NB600-276] - Detection of human Leo1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

LEO1 Antibody Summary

Immunogen
A synthetic peptide which maps to a region between residues 550 and 600 of human senescence downregulated leo1-like using the numbering given in TrEMBL entry Q8WVC0 (GeneID 123169).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LEO1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 2-8 ug/mg lysate
  • Western Blot 1:1000-1:5000
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Control
LEO1 Overexpression Lysate
Publications
Read Publications using NB600-276.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for LEO1 Antibody

  • Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
  • RDLReplicative senescence down-regulated leo1-like protein
  • RNA polymerase-associated protein LEO1

Background

Leo1 is one of four subunits of the Paf complex, which has been shown to aid in the regulation of transcriptional elongation, histone methylation and RNA processing in yeast.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for LEO1 Antibody (NB600-276)(2)

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Product General Protocols

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Video Protocols

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for LEO1 Antibody (NB600-276)

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Bioinformatics

Gene Symbol LEO1
Entrez
Uniprot