| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-16704-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89423-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86236-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18660-25ul | |||
![Western Blot: LEO1 Antibody [NBP3-16704] - Western blot analysis of extracts of various cell lines, using LEO1 antibody (NBP3-16704) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/LEO1-Antibody-Western-Blot-NBP3-16704-img0006.jpg "Western Blot: LEO1 Antibody [NBP3-16704] - Western blot analysis of extracts of various cell lines, using LEO1 antibody (NBP3-16704) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunocytochemistry/Immunofluorescence: LEO1 Antibody [NBP3-16704] - Immunofluorescence analysis of U2OS cells using LEO1 Rabbit pAb (NBP3-16704) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/LEO1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-16704-img0005.jpg)
![Immunohistochemistry-Paraffin: LEO1 Antibody [NBP3-16704] - Immunohistochemistry of paraffin-embedded mouse liver using LEO1 Rabbit pAb (NBP3-16704) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/LEO1-Antibody-Immunohistochemistry-Paraffin-NBP3-16704-img0003.jpg)
![Western Blot: LEO1 Antibody [NBP3-16704] - Western blot analysis of extracts of various cell lines, using LEO1 antibody (NBP3-16704) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/LEO1-Antibody-Western-Blot-NBP3-16704-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: LEO1 Antibody [NBP3-16704] - Immunofluorescence analysis of NIH/3T3 cells using LEO1 Rabbit pAb (NBP3-16704) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/LEO1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-16704-img0004.jpg)
![Immunohistochemistry-Paraffin: LEO1 Antibody [NBP3-16704] - Immunohistochemistry of paraffin-embedded human colon carcinoma using LEO1 Rabbit pAb (NBP3-16704) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/LEO1-Antibody-Immunohistochemistry-Paraffin-NBP3-16704-img0001.jpg)
![Immunohistochemistry-Paraffin: LEO1 Antibody [NBP3-16704] - Immunohistochemistry of paraffin-embedded human esophageal cancer using LEO1 Rabbit pAb (NBP3-16704) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/LEO1-Antibody-Immunohistochemistry-Paraffin-NBP3-16704-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: LEO1 Antibody - Azide and BSA Free [NBP3-16704] - Immunofluorescence analysis of NIH/3T3 cells using LEO1 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16704_rabbit-leo1-pab-52202511392245.jpg)
![Immunocytochemistry/ Immunofluorescence: LEO1 Antibody - Azide and BSA Free [NBP3-16704] - Immunofluorescence analysis of U2OS cells using LEO1 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16704_rabbit-leo1-pab-52202511383788.jpg)
![Immunocytochemistry/ Immunofluorescence: LEO1 Antibody - Azide and BSA Free [LEO1] - Immunofluorescence analysis of U2OS cells using LEO1 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16704_rabbit-leo1-pab-52202517315546.jpg)
![Immunocytochemistry/ Immunofluorescence: LEO1 Antibody - Azide and BSA Free [LEO1] - Immunofluorescence analysis of NIH/3T3 cells using LEO1 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16704_rabbit-leo1-pab-52202517311088.jpg)
![Western Blot: LEO1 Antibody [NB600-276] - Detection of mouse Leo1 by western blot. Samples: Whole cell lysate (15 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Leo1 antibody [NB600-276] used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/LEO1-Antibody-Western-Blot-NB600-276-img0008.jpg "Western Blot: LEO1 Antibody [NB600-276] - Detection of mouse Leo1 by western blot. Samples: Whole cell lysate (15 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Leo1 antibody [NB600-276] used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry: LEO1 Antibody [NB600-276] - Detection of mouse Leo1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-Leo1 (NB600-276). Detection: DAB](http://images.novusbio.com/fullsize/LEO1-Antibody-Immunohistochemistry-NB600-276-img0006.jpg)
![Western Blot: LEO1 Antibody [NB600-276] - Detection of human Leo1 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, MCF-7, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Leo1 antibody [NB600-276] used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/LEO1-Antibody-Western-Blot-NB600-276-img0007.jpg)
![Immunohistochemistry: LEO1 Antibody [NB600-276] - Sample: FFPE section of human stomach carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-LEO1 used at a dilution of 1:5,000 (0.2ug/ml) and 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/LEO1-Antibody-Immunohistochemistry-NB600-276-img0005.jpg)
![Immunoprecipitation: LEO1 Antibody [NB600-276] - Detection of human Leo1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Leo1 antibody [NB600-276] (lot 3) used for IP at 6 ug per reaction. Leo1 was also immunoprecipitated by a previous lot of this antibody (lot 2). For blotting immunoprecipitated Leo1, NB600-276 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/LEO1-Antibody-Immunoprecipitation-NB600-276-img0009.jpg)
![Western Blot: LEO1 Antibody [NBP1-89423] - Analysis in control (vector only transfected HEK293T lysate) and LEO1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/LEO1-Antibody-Western-Blot-NBP1-89423-img0009.jpg "Western Blot: LEO1 Antibody [NBP1-89423] - Analysis in control (vector only transfected HEK293T lysate) and LEO1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: LEO1 Antibody [NBP1-89423] - Staining of human cell line U-251 MG shows localization to nucleoplasm, nucleoli fibrillar center & centrosome. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/LEO1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89423-img0007.jpg)
![Immunohistochemistry-Paraffin: LEO1 Antibody [NBP1-89423] - Staining of human rectum shows nuclear and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/LEO1-Antibody-Immunohistochemistry-Paraffin-NBP1-89423-img0008.jpg)
![Western Blot: LEO1 Antibody [NB100-79777] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiLeo1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/LEO1-Antibody-Western-Blot-NB100-79777-img0004.jpg "Western Blot: LEO1 Antibody [NB100-79777] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiLeo1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: LEO1 Antibody [NBP1-86236] - Staining of human rectum shows moderate nuclear and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/LEO1-Antibody-Immunohistochemistry-Paraffin-NBP1-86236-img0005.jpg "Immunohistochemistry-Paraffin: LEO1 Antibody [NBP1-86236] - Staining of human rectum shows moderate nuclear and cytoplasmic positivity in glandular cells.")
![Western Blot: LEO1 Antibody [NBP2-19346] - Sample (30 ug of whole cell lysate) A: Jurkat 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/LEO1-Antibody-Western-Blot-NBP2-19346-img0001.jpg "Western Blot: LEO1 Antibody [NBP2-19346] - Sample (30 ug of whole cell lysate) A: Jurkat 5% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: LEO1 Overexpression Lysate (Adult Normal) [NBL1-12488] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for LEO1.](http://images.novusbio.com/fullsize/LEO1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12488-img0002.jpg "Western Blot: LEO1 Overexpression Lysate (Adult Normal) [NBL1-12488] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for LEO1.")
![Western Blot: LEO1 Antibody [NBP3-18638] - Figure 1. Western blot analysis using the antibody directed against hLEO1 Nuclear extracts from HeLa cells (20 ug) were analysed by western blot using the antibody against hLEO1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/LEO1 Antibody-Western Blot-NBP3-18638-img0001.jpg "Western Blot: LEO1 Antibody [NBP3-18638] - Figure 1. Western blot analysis using the antibody directed against hLEO1 Nuclear extracts from HeLa cells (20 ug) were analysed by western blot using the antibody against hLEO1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.")
![Western Blot: LEO1 Antibody [NBP3-18660] - Figure 1. Western blot analysis using the antibody directed against hLEO1 To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against human JMJD2c . The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000.](http://images.novusbio.com/fullsize/LEO1 Antibody-Western Blot-NBP3-18660-img0001.jpg "Western Blot: LEO1 Antibody [NBP3-18660] - Figure 1. Western blot analysis using the antibody directed against hLEO1 To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against human JMJD2c . The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000.")